Letno poročilo 2005

enokomponentno mobilno fazo, kar je
bistvena prednost v primerjavi z objavljenimi
metodami. Tako nismo dolo~ili samo skupne
vsebnosti likopena v razli~nih vzorcih, ampak
tudi vsebnost izomer.
- S partnerji z University of Helsinki (Faculty of
Pharmacy), Finska, smo dokon~ali delo o TLC
lo~bi katehinov. S partnerji s Palacky University
(Faculty of Medicine, Institute of Medical
Chemistry and Biochemistry), Olomuc, ^e{ka,
pa smo sodelovali pri raziskovanju biolo{ke
in kemijske variabilnosti jakuna (Smalanthus
sonchifolius). Sodelavci z In{tituta za
hmeljarstvo in pivovarstvo iz @alca so nam
pripravili prve vzorce korenin rastlin iz dru`ine
srhkolistnic, ki naj bi vsebovali zdravilne
naftokinone. S sodelavci z Biotehni{ke
fakultete (Oddelek za agronomijo) Univerze
v Ljubljani smo naredili preliminarne poskuse
z ekstrakcijo snovi s povr{in listov zelja. Zelje
se je razlikovalo po naravni odpornosti proti
dolo~enim {kodljivim `u`elkam. Prvi rezultati
so pokazali, da med odporno in neodporno
vrsto zelja obstajajo razlike na molekularnem
nivoju.
- V okviru sodelovanja s skupino z University
of Belgrade (Faculty of Pharmacy), Srbija in
^rna gora, smo razvili HPLC metodo za
dolo~anje fluorokinolonskega antibiotika
norfloksacina v tabletah in metodo za
dolo~anje ne~istot oziroma razgradnih
SLIKA 2
Prvi prehrambeni izdelki z dodatkom vodotopnega
Q10 izdelani po licenci Kemijskega in{tituta
Laboratorij za prehrambeno kemijo
Laboratory for Food Chemistry
that differed in their natural resistance against
chosen harmful insects. First analytical results
showed that differences between resistant
and unresistant species exist on molecular
level.
- Within the cooperation with the group from
University of Belgrade (Faculty of Pharmacy),
Belgrade, Serbia and Montenegro, we developed
a method for the determination of
fluoroquinolone antibiotic norfloxacin in tablets
and a method for the determination of
impurities and degradation products in tablets
with norfloxacin as the active ingredient.
We studied conditions for TLC densitometric
determination of norfloxacin by means of UV
absorption or natural fluorescence of the
compound.
- Within the cooperation with the group from
Medical Centre Ljubljana, Slovenia, we investigated
the influence of enteral nutrition (with
different dietary supplements with specific
action on the digestive organs) on intestinal
permeability and healing in multiple injured
patients. Intestinal permeability was evaluated
by determination of lactulose and mannitol
in 43 urine samples. The ratio of
lactulose/mannitol excretion in urine after
their administration is of great importance
for evaluation of malabsorption and intestinal
permeability disruption in some diseases.
FIGURE 2
The first group of food products with water soluble
Q10 prepared according to the license of National
Institute of Chemistry
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