Letno poročilo 2005

medicino in bioznanosti Raziskovalnega centra
Borstel v Nem~iji. Pape`evo sepso, kot ve~ino
vseh primerov sepse, je povzro~il endotoksin iz
gram-negativnih bakterij. Imunski odziv in
uporaba antibiotikov lahko iz propadajo~ih
bakterij sprostita endotoksin. Zdravilo, ki bi
lahko po eni strani uni~ilo gram-negativne
patogene in isto~asno nevtraliziralo endotoksin,
je neke vrste sveti gral na podro~ju raziskav
sepse. Sedaj Roman Jerala in sodelavci s
Kemijskega in{tituta, Ljubljana, Slovenija
poro~ajo o sintezi novih spojin, ki zdru`ujejo ti
dve lastnosti.
»To delo prispeva k znanju o za{~itnem
delovanju tovrstnih spojin, ki bi lahko bile
SLIKA 2:
Fluorescen~ni preto~ni celi~ni sorter omogo~a
lo~evanje celic na osnovi njihovih lastnosti in tudi
isto~asne meritve ve~jega {tevila citokinov v raztopini
na osnovi principa multipleksinga. Ta naprava
omogo~a izolacijo homogenih celi~nih frakcij in
analizo izra`anja posameznih celi~nih markerjev.
In{trument je bil kupljen s sodelovanjem L11, L12 in
konzorcija slovenskih raziskovalnih organizacij
Laboratorij za biotehnologijo
Laboratory of Biotechnology
Klaus Brandenburg of the Leibniz-Center for
Medicine and Biosciences, Research Center
Borstel, Borstel, Germany. The Pope’s sepsis, like
most, was induced by endotoxin from gramnegative
bacteria. Immune reaction and antibiotics
can both cause dying bacteria to release
endotoxin. A drug that could both kill gramnegative
pathogens and neutralize endotoxin
has been a holy grail. Now Roman Jerala and
colleagues of the National Institute of Chemistry,
Ljubljana, Slovenia, report the synthesis of
novel oleoylamines that combine the two properties.
This work “adds to the growing body of
literature on the protective effects of such compounds
that may be of prophylactic value in
FIGURE 2:
Fluorescence cell sorter allows the separation of cells
based on their specific properties as well as quantification
of several components in the solution based
on the multiplexing. The instrument allows the separation
of homogeneous cell fractions and analysis of
the level of expression of specific cellular markers.
The instrument was purchased by L11, L12 and the
consortium of Slovenian research organizations.
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