Proceedings of the International Cyanide Detection Testing Workshop

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1:30 Overview of and Potential Cyanide Testing Methods • Application of the IMA method Benita Manipula • Ion-selective Electrodes for Cyanide: Present Status and Future Possible Improvements Dr. Martin Frant • Enzymatic biosensor method Dr. Karen Mak • Use of biomarkers to detect cyanide impacts Dr. Craig Downs • Rapid fi eld test procedure Dr. Peter Rubec • 3:15 Break The realm of possibilities for cyanide detection Dr. Brian Logue 3:30 Discussion: Factors to identify appropriate tests for All detection of cyanide in marine fi shes 5:00 Wrap up and adjourn for the day 6:30 Dinner Friday February 8 8:30 Re-cap of Previous Day and Agenda Review Dr. Bob Kobelski 8:45 Charge to Breakout Groups Keystone 8:50 Convene Breakout Group Discussions All • Detection Methods • Export Country • Import Country 10:30 Break items available 11:30 Breakout Group Status Report/Interim Summary All 12:00 Lunch 1:00 Resume Breakout Group Discussions 2:45 Break 3:00 Breakout Group Reports and Recommendations 3:30 Discussion All 4:30 Next Steps and Wrap Up • Future directions and Closing Thoughts Dr. Andy Bruckner 160
Gil Adora Assistant Director Philippines Bureau of Fisheries and Aquatic Resources Quezon City, Metro Manila, Philippines gi_adora@yahoo.com Andy Bruckner, Ph.D. Coral Reef Ecologist Coral Reef Conservation Program US National Oceanographic and Atmospheric Administration Silver Spring, Maryland, USA andy.bruckner@noaa.gov Ferdinand Cruz Executive Director East Asian Seas and Terrestrial Initiatives Los Banos City, Laguna, Philippines cruzferdinand@gmail.com Agus Dermawan Director of Conservation and Marine National Park Directorate General of Coastal and Small Islands Ministry of Marine Affairs and Fisheries, Indonesia Jakarta, Indonesia agusder81@yahoo.com Martin Frant, Ph.D. Research Director Thermo-Fisher Inc. Beverly, Massachusetts, USA ms.frant2@verizon.net APPENDIX II List of Participants 161 Thu Ho Vice Director and Program Manager, Coral Reefs Center for Marinelife Conservation Hanoi, Vietnam thu@mcdvietnam.org Aquilino Alvarez Policy Advisor for the Director Philippines Bureau of Fisheries and Aquatic Resources Quezon City, Metro Manila, Philippines linalvar@yahoo.com Tomas Cabagay Vice-President East Asian Seas and Terrestrial Initiatives Los Banos, Laguna, Philippines tomcabagay@gmail.com Patty Debenham, Ph.D. Director, Coral Program SeaWeb San Francisco, California, USA pdebenham@seaweb.org Ken Goddard Laboratory Director US Fish and Wildlife Service National Forensics Laboratory Ashland, Oregon, USA ken_goddard@fws.gov
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Proceedings of the International Cy
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Proceedings of the Cyanide Detectio
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ACKNOWLEDGEMENTS The Proceedings of
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PREFACE The International Cyanide D
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EXECUTIVE SUMMARY The International
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ATCA) in homogenized fi sh tissue m
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Validation of the ISE method applie
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Importing countries should also req
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INTRODUCTION Cyanide fi shing is a
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through the implementation of a net
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Working Group 2 The Export Working
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o o o o Can the U.S. require verifi
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Polymeric membrane based ion select
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Goal 2: Provide recommendations on
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hands-on training that develops a d
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Goal 3: Identify research needs to
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Working Group 1 Participants Bob KO
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estimated at roughly 3 to 5 days. H
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Goal 2 : Identify steps, agreements
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3) certifi cate, but tests positive
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Report from Working Group 3 The Par
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No. of Shipments 6,000 5,000 4,000
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Goal 3: Provide a recommendation wi
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Working Group 3 Participants Eric P
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Introduction Since the early 1960
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ainbow trout exposed to 40 mg SCN -
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Blennies, which were net-caught and
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electrical potential (similar to an
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in the Philippines under contract w
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endorsing the IMA’s use of the IS
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The intended products from the rese
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WPCF 1992, 1998). Both of these stu
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ASTM (1997) Standard test methods f
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Kuegsen, M., Kloock, J.P., Knobbe,
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Philippines. In Proceedings from th
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3) 4) 5) 6) the metabolism of cyani
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or new matrices. 5.2.3 Modifi catio
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Level Two: The method should be val
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of cyanide exposure and to identify
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Special considerations for the anal
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tissue samples indispensable. Altho
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y reduction and subsequent separati
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can also be used for analysis of cy
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thiocyanate in saliva and serum bas
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are many discrepancies in the liter
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Table 3. Analytical Methods to dete
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Poisoning, In Textbook of military
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(70) Odoul, M., Fouillet, B., Nouri
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V. (1975) Stable reagents for the c
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(159) Felscher, D. and Wulfmeyer, M
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Purpose of this Document The purpos
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day. One cyanide tablet (2 g) is mi
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10-90% following exposure to cyanid
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existing cyanide detection methods
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- Plasma lactate — elevated plasm
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Method Advantages Disadvantages Hig
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Paper Method Matrix Detection Limit
Page 119 and 120: Table 4 lists experts identifi ed t
Page 121 and 122: X Jerry Thomas CDC Em Steve Borron
Page 123 and 124: References Barber, C. and Pratt, V.
Page 125 and 126: Rates of Recovery. Environmental Ma
Page 127: COUNTRY REPORTS
Page 130 and 131: The first three of these destructiv
Page 132 and 133: enforcement is minimal. There is an
Page 134 and 135: 1. Management/guidance to the Minis
Page 136 and 137: in Vietnam now and requires more ef
Page 138 and 139: Figure 2: Administrative structure
Page 140 and 141: References Baquero, J. (1999) Marin
Page 142 and 143: Acronyms • CDT • Cyanide Detect
Page 144 and 145: Law/Ordinance/Decree /Decision 52/2
Page 146 and 147: of samples required for analysis. U
Page 148 and 149: Field test kit. BFAR recognizes the
Page 150 and 151: Political Will Having planted the s
Page 152 and 153: gravely by fi shing communities. Th
Page 154 and 155: ecome the partner and counterpart o
Page 157 and 158: Ion-Selective Electrodes for Cyanid
Page 159 and 160: References Alban, J., Manipula, B.E
Page 161 and 162: levels with mV readings recorded as
Page 163 and 164: Trends Determined by Cyanide Testin
Page 165 and 166: Implementing agencies: • Quaranti
Page 167: APPENDIX
Page 172 and 173: Kristine Johnson Director Kingfi sh
Page 174: Assessment of U.S. Role in Trade: U
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