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Assessment of Conversion Technologies for Bioalcohol Fuel ...

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CATEGORY X–OTHER BIOLOGICAL PROCESSES<br />

Genotypes, Inc., Pacifica, Cali<strong>for</strong>nia<br />

Organizational Background–Genotypes, Inc. is a small Cali<strong>for</strong>nia firm founded in<br />

1992 as a contract research company to assist biotech/pharmaceutical companies<br />

with yeast strain improvement. Its principals are experienced biochemists with<br />

extensive backgrounds in the biotechnology industry. Since 1996, Genotypes has<br />

been pursuing development <strong>of</strong> a novel technology approach to producing ethanol from<br />

solar energy in shallow ponds employing specialty cultured organisms. The company<br />

has filed several patents on its technology, beginning in 1998, involving bioengineering<br />

the desired organism to photosynthetically produce ethanol in ponds.<br />

Technology Characteristics–The Genotypes technology, shown in Figure A21,<br />

involves use <strong>of</strong> a bioengineered photosynthetic (nitrogen-fixing) organism–<br />

cyanobacteria stabilized as organelles in yeast–to produce ethanol in one meterdeep<br />

ponds using only solar energy, water, atmospheric carbon dioxide and trace<br />

minerals. Biomass would be produced during the growth <strong>of</strong> the organism up to an<br />

appropriate density, then the biomass production would be essentially turned <strong>of</strong>f and<br />

replaced by direct conversion <strong>of</strong> photosynthetically produced sugars to ethanol. Thus<br />

the organism would produce its own biomass feedstock, resulting in no net carbon<br />

emissions since carbon dioxide taken up to produce sugars, which are directly<br />

converted to ethanol in the organism, would be released by burning the alcohol but<br />

then reabsorbed upon making more ethanol in the same organism. Genotypes<br />

estimates the potential ethanol yields from this process to be in the range <strong>of</strong> 37,000<br />

gallons per acre per year. This would translate, <strong>for</strong> example, into a land area<br />

requirement <strong>of</strong> about 670 square miles to produce the ethanol equivalent <strong>of</strong><br />

Cali<strong>for</strong>nia’s curent gasoline supply. Genotypes also estimates a potential ethanol<br />

production cost from its pond technology could eventually be as low as $0.33 per<br />

gallon.<br />

The advantages claimed <strong>for</strong> this unique technological approach are: 1) Scaleable–<br />

would use less than 1% <strong>of</strong> land that corn ethanol uses - could eventually be scaled to<br />

completely replace gasoline. 2) Cost effective: scaled-up projections <strong>of</strong> less than<br />

$1.00/gallon. 3) Carbon Neutral (no net carbon dioxide put in the air)–<br />

environmentally friendly. 4) Sustainable–will not run out <strong>of</strong> feedstocks: sunshine,<br />

carbon dioxide, and trace minerals. Also, the pond system is considered highly<br />

adaptable to desert-type climates and areas not well-suited <strong>for</strong> conventional<br />

agriculture or bioenergy crops.<br />

Development Status–Genotypes has conducted laboratory research aimed at<br />

developing the best photosynthetic organism <strong>for</strong> ethanol production at its <strong>for</strong>mer<br />

laboratory in South San Francisco. The laboratory was sold in 2000, and Genotypes<br />

continues to see partnerships with other developmental organizations and/or funding<br />

to carry on this development work. The company has delivered a number <strong>of</strong><br />

111

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