final program.qxd - Parallels Plesk Panel

PP 2.3
Broad neutralization of Human Immunodeficiency Virus Type 1 by monoclonal
antibody against C2 region
Apichai Sreepian, Chongrak Permmongkol, Nattaya Tanliang, Ruengpung Sutthent
Department of Microbiology, Faculty of Medicine Siriraj Hospital, and Faculty of Medical
Technology, Mahidol University, Bangkok, Thailand
The linear peptide corresponding to conserved region C2 on gp120 was previously
designed and synthesized as a short peptide (amino acid position 218-239). Then the
activity of peptide was investigated and shown to inhibit neutralization activity of long-term
nonprogressor sera against HIV-1 CRF01_AE primary isolates (PI) from Thailand
(Sreepian et al., 2004). Consequently, we produced monoclonal antibody against C2
peptide (mAbC2) by conventional method and investigated activity of this mAb by
neutralizing laboratory strains of HIV-1; MN, IIIB and NPO3. We found that it could
neutralize all laboratory strains approximately (IC50) at concentration 155.65+15.9 µg/ml.
Furthermore, more primary isolates collected from Thailand have been investigated and
found to be neutralized by mAbC2 at concentration 96+53.85 µg/ml. This study showed
that mAbC2 could neutralize broadly laboratory strains and primary isolates and C2
epitope might be a good candidate for vaccine.
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