final program.qxd - Parallels Plesk Panel
final program.qxd - Parallels Plesk Panel
final program.qxd - Parallels Plesk Panel
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PP 2.5<br />
Evaluation of the Trugene GP41HIV-1 genotyping Kit (Bayer) in comparison with<br />
ANRS recommended method<br />
Vanna Geromel( 1 ), Patricia Pinson-Recordon ( 2 ), Bernard Masquelier ( 2 ), Hervé Fleury ( 2 ),<br />
Jean-Dominique Poveda( 1 )<br />
1<br />
Laboratoire Pasteur Cerba, 95066 Cergy Pontoise cedex 09, France<br />
2<br />
Laboratoire de Virologie, CHU de Bordeaux, Groupe hospitalier Pellegrin, 33076<br />
Bordeaux cedex, France<br />
Objectives<br />
To compare the results obtained with the TruGene GP41 HIV-1 genotyping kit (Bayer) with<br />
the ANRS reference method on plasmas of HIV-1 infected patients from differents<br />
sub-types, on enfuvirtide (T20)-experienced and -not experienced patients.<br />
Methods<br />
22 plasma samples (10 subtype B, 8 subtype C, 4 subtype unknown) taken from 22<br />
patients, previously tested with recommended ANRS primers for HR1-HR2 region<br />
(www.hivfrenchresitance.org) and 7 samples from the ANRS annual proficieny panel (3<br />
subtype B, 3 subtype G, 1 subtype J, 1 negative) were blindly tested with the TruGene<br />
GP41 HIV-1 genotyping Kit (Bayer) according to the manufacturer's recommendations.<br />
Complete nucleotide and amino-acid sequences obtained were compared and mutations<br />
in the 36-45 amino-acids positions of HR1, clinically relevant according the ANRS 2005<br />
interpretation algorithm, were specifically investigated.<br />
Results<br />
All samples previously genotyped with ANRS primers were successfully amplified with the<br />
Trugene GP41 HIV-1 genotyping kit. One sample from the ANRS proficiency panel was<br />
negative with both methods.<br />
A concordance of 99.5% was obtained on the 5250 nucleotide positions of the 230 bp<br />
sequence assessed, and a concordance of 97.4% was obtained for the 189 amino-acids<br />
positions tested. A mixture (wild type/mutant) was found at 2 positions with the ANRS<br />
method and in 3 with the TruGene kit when the alternative method found either the wild<br />
type or the mutated amino-acid. No position was found to be a pure mutation with one<br />
method and a pure wild-type with the other. No discrepancy was obtained between the two<br />
methods in terms of resistance to T20 according to interpretation with the ANRS 2005<br />
algorithm. Mutations were found only in T20 experienced patients.<br />
POSTERS<br />
Conclusions<br />
The TruGene GP41 HIV-1 genotyping kit (Bayer) was very well correlated to the ANRS<br />
method taken as reference. It can be considered as suitable to a routine clinical use.<br />
“ Focusing FIRST on PEOPLE “ 123 w w w . i s h e i d . c o m
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