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Viral shedding of avian influenza virus in recovered patients from Thailand.
Sontana Siritantikorn, Wannee Kantakamalakul, Kulkanya Chokpaibulkit, Suksan
Assanasen, Nawin Horthongkham, Duangnapha Arwon, Kawinyanee Loengaram,
Ruengpung Sutthent
Department of Microbiology, Pediatric, Medicine, Faculty of Medicine Siriraj Hospital,
Mahidol University, Thailand.
Background
In 2005, there were 5 human infected cases of avian influenza (H5N1) reported from
Thailand, 3 cases were admitted at Siriraj Hospital and alive. In order to improve
diagnostic and infection control of avian influenza virus in human, the virus from
nasopharyngeal secretion and feces of these survivals were studied.
Methods
The nasopharyngeal wash (NPW) and feces were collected alternative day. Avian
influenza virus was detected by reverse transcriptase PCR (RT-PCR) and culture in
MDCK cell line.
Results
The duration of virus excretion in NPW and stool detected by RT-PCR was 17.3 (16-19)
and 8 (7-9) days after the day of onset, respectively. The phylogenetic analysis of
hemagglutinin gene from these isolates were clustered with the first avian influenza virus
human isolate from Thailand (A/Thailand/1(KAN-1/04HA). There was no genetic
compartmentalization of avian influenza virus in both clinical samples.
Conclusions
The presence of avian influenza virus in human feces will have effect on infection control.
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