final program.qxd - Parallels Plesk Panel

PP 5.2
Detection of HIV/HCV Co-infection Markers in Blood and Saliva
Alexey I. Mazus, Alexander Y. Olshanskiy, Irina A. Simonova, Alexander A. Goliusov,
Yuri Martinov
Moscow Centre for HIV/AIDS Prevention and Treatment, 5-15 8th Sokolinoy Gory Str.,
Moscow 105275, Russia; Moscow State University of Medicine and Dentistry, 20/1
Delegatskaya Str., 103473, Moscow, Russia
We investigated matched plasma and saliva samples from 34 HIV-infected individuals. 22
of these patients were co-infected with HCV. 13 patients were on HAART.
We detected 1) the viral load (RNA HIV-1, RNA HCV) by PCR; 2) HIV-1,2 antibodies by
ELISA and Western blot; 3) antibodies to core, NS3, NS4, NS5 antigens HCV by ELISA.
We found correlation between HIV viral load in plasma and saliva. 18% of plasma samples
showed the viral load < 400 copies/ml, while for the remaining 82 % (28 patients) it
ranged from 526 to 215000 copies/ml. The HIV viral load was < 400 copies/ml in 13
saliva samples (42%), and ranged from 403 to 263,000 copies/ml in the other 18 samples
(58%).
The correlation coefficient shows direct correlation between HIV viral load in blood and
saliva (r = +0.3925±0.14 t=2.78. p