final program.qxd - Parallels Plesk Panel

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Analysis of plasma cytokines in Immunological and virological discordant HIV-1
infected patients by Microarray system: A pilot study.
Naresh Sachdeva 1 , H S Yoon 1 , Kyoko Oshima 1 , Luis Cintron 2 , Domingo Garcia 2 ,
Karl Goodkin 1 and Deshratn Asthana 1 .
1
University of Miami and 2Borinquen Health care Center, Florida, USA.
Background and Objectives
There are some HIV infected patients in whom complete suppression of the viral load
does not assure restoration of CD4+ T cell counts. Such discordant responses suggest
that there are other factors in immune system that influence the restoration of CD4+ T cell
numbers despite changes in the plasma HIV-1 levels. It is well established that the
combined effect of multiple cytokines and chemokines in the immune response to
disease or immune modulation by drugs is often more important than the function of
specific cytokines. Therefore, the plasma cytokine milieu might be significant in influencing
the immune response to HIV-1 during the course of infection. In this study we have used
a commercially available biochip microarray system capable of measuring 12 cytokines
(IL-2, IL-4, IL-6, IL-8, IL-10, IL-1α, IL-1ß, IFN-γ, TNF-α, MCP-1, VEGF, and EGF) in
Immunological and virological discordant HIV-1 infected subjects to find out differences if
any in their cytokine profiles compared to concordant HIV-1 infected individuals.
Methods
EDTA plasma samples were obtained from 25 discordant, 25 concordant HIV patients and
12 normal healthy individuals. A sandwich chemiluminescent assay was performed with
100 µl of plasma sample using reagents (including the calibrators and controls) and
protocols supplied by the same manufacturer. Cytokine levels between study subjects
were compared by paired samples t- tests using SPSS software (version 3.0).
Results
Overall there appeared to be a significant difference in the levels of TNF-α, MCP-1 and
EGF between the patients versus the normal healthy controls (p