final program.qxd - Parallels Plesk Panel
final program.qxd - Parallels Plesk Panel
final program.qxd - Parallels Plesk Panel
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OP 5.1<br />
Genetic Analysis of the Env Gene of Human Immunodeficiency Virus Type 1(HIV-1)<br />
Strains from Patients in Cyprus from 1994 Until 2005: Evidence of a Highly Evolving<br />
Epidemic<br />
Ioanna Kousiappa, Alina Giantsiou-Kyriacou, Efthymia Yiacoumi, Victoria Demetriou and<br />
Leondios G. Kostrikis<br />
Department of Biological Sciences, University of Cyprus, 75 Kallipoleos Avenue, P.O.Box<br />
20537, CY-1678, Nicosia, Cyprus<br />
ABSTRACTS<br />
Background<br />
The aim of this research was the investigation of the genetic variation of newly arrived<br />
HIV-1 strains that were isolated from Cypriot patients in 2005 and the comparison of the<br />
results with those obtained from a corresponding study carried out in 1994.<br />
Material and Methods<br />
Peripheral blood mononuclear cell (PBMC) samples were extracted from the blood of 141<br />
HIV-1 patients with their informed consent. DNA was extracted and the C2-C5 region of<br />
the Env gene was amplified by means of nested PCR and sequenced using the ABI 3130<br />
Genetic Analyzer. Sequence alignment was done through ClustalX and genetic subtypes<br />
were identified by phylogenetic analysis using neighboring-joining with Kimura's<br />
two-parameter method.<br />
Results<br />
The phylogenetic analysis of 137 samples reveals that 69% of patients have the subtype<br />
B, 19% ?, 8% C, 1,5% F, 1% D and 1% CRF04_cpx. 1.5% of the samples were not<br />
recognized. In an analogous investigation in 1994, 24 strains from Cypriot patients were<br />
studied and the results were 63% ?, 17% ?CY, 4% C, 8% FCY and 8% CRF04-cpx<br />
exhibiting multiple introductions of subtype B and unique introductions of subtypes ?,<br />
C, F and the CRF04_cpx.<br />
Conclusion<br />
These newly found data demonstrate a heterogeneous epidemiological status of HIV-1 in<br />
Cyprus. Subtype B is the most dominant with a wider range of strain variation compared<br />
with the other subtypes, showing multiple introductions from other geographical regions.<br />
Compared to the 1994 study, the number and the variation of the strains of subtypes A and<br />
C have increased due to introductions of new ones. The ?CY, FCY subgroups and the<br />
CRF04_cpx that were detected in 1994 did not spread further. In the present study, a new<br />
introduction of subtype F has appeared and a strain of subtype D has been found for the<br />
first time.<br />
OP 5.2<br />
Therapy and New ARV Interactions<br />
No abstract available<br />
“ Focusing FIRST on PEOPLE “ 49 w w w . i s h e i d . c o m
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