Publications:Bigot S, Lucas L, Morrow P, Mitchell CA, Saetzler K; Estimating Leukocyte Velocities from High-speed 1d Line ScansOriented Orthogonal to Blood Flow; ISBI 2007: 376-379, 2007Rutland CS, Mitchell CA, Nasir M, Konerding MA, Drexler HCA; Microphthalmia, persistent hyperplastic hyaloidvasculature and lens anomalies following over-expression <strong>of</strong> VEGF-A 188from the aA-crystallin promoter; MolecularVision, 13: 47-56, 2007Lucas LAG, S Pop SR, Machado MJC, Ma YL, Waters SL, Richardson G, Saetzler K, Jensen OE and Mitchell CA;Experimental and theoretical modelling <strong>of</strong> blind-ended vessels within a developing angiogenic plexus; Microvascular<strong>Research</strong>, 76: 161-168, 2008Lee PD, Atwood RC, Rockett P, Konerdingn MA, Jones JR, Mitchell CA; Proceedings <strong>of</strong> SPIE, Vol. 7078, 70780E, 2008[DOI:10.1117/12.795558]<strong>Research</strong> Staff:Dr Barry O’Hagan<strong>Research</strong> FellowContact Details:T: +44 (0)28 70324765bmg.ohagan@ulster.ac.ukDr O’Hagan’s main <strong>research</strong> interests and activities involve the use and development <strong>of</strong> advanced imaging systemsfor use in a variety <strong>of</strong> biological <strong>research</strong> projects.He is responsible for the routine maintenance, alignment and operation <strong>of</strong> the FEI Centre for Advanced Imaging(electron optics including transmission electron microscopy, Environmental scanning electron microscopy, Cry<strong>of</strong>ocused ion beam microscopy). He liaises closely with FEI field engineers and customer support in fault diagnosisand repair. Development and optimisation <strong>of</strong> cryo dual beam microscopy for biological samples is the main thrust <strong>of</strong>his work and is ongoing. This includes developing protocols for sample preparation and imaging parameters for bothambient and cryogenic conditions, and have working ‘recipes’ for chemically fixed and unfixed cellular monolayers,resin embedded material and cryo-fractured samples.24
He has trained a large number <strong>of</strong> other users in the use <strong>of</strong> optical and electron microscopy to the point where theyare capable <strong>of</strong> using the instrumentation unsupervised or with minimal supervision.Dr O’Hagan’s first doctoral <strong>research</strong> student has recently finished her program <strong>of</strong> work and is awaiting a viva vocein the near future.Project collaborations:In addition to the ongoing development <strong>of</strong> biological cryo dual beam microscopy, Dr O’Hagan has been closelyinvolved in a number <strong>of</strong> other <strong>research</strong> projects within <strong>Ulster</strong> <strong>University</strong>, as well as collaborations with externalcompanies and institutions. These include the following:• Transmission electron microscopy <strong>of</strong> Nanotube structure (NI Bioengineering Centre - NIBEC).• Cryo-Dualbeam microscopy <strong>of</strong> osteoblast response to nanogrooved substrates. (<strong>University</strong> Nijmegen MedicalCentre, joint publication pending).• FIB nano-machining <strong>of</strong> AFM probe tips (Yael Dror, Oxford).• Cryo FIB, variable pressure ESEM Mitochondrial imaging. (<strong>University</strong> <strong>of</strong> Leicester, joint publication pending).• Temperature variation in SEM chamber during cryo operation (NIBEC).• TEM polymer structure and formation (Chemistry at Queens <strong>University</strong>).• Assistance with Nanotoxicology <strong>research</strong> (NanoInteract).• Assistance with fat emulsion <strong>research</strong> (CAST award with Unilever <strong>Research</strong>, Vlaardigan).• Assistance with investigations into bacterial adhesion to cells (CAST award with Unilever <strong>Research</strong>, PortSunlight).• Preliminary imaging <strong>of</strong> oil bearing rock core samples. (collaboration with Corex).Fig. 1Cryo Dual beam cross section through malariainfected bloodFig. 2Deconvolved multi-fluorescence image <strong>of</strong>mammalian cell25
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Dr Barnes has developed expertise i
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Dr Alison GallagherSenior Lecturer
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In addition, results of a pilot stu
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Dr Maeve KerrResearch AssociateCont
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Memberships of External Committees/
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Indicators of Esteem:Professor McNu
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were examined. The intervention and
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Within this work both short-term an
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micronutrient supplementation at a
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10. STEM CELL & EPIGENETICS RESEARC
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Publications:Lees-Murdock DJ, Lau H
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Publications:Lees-Murdock DJ, Lau H
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11. SYSTEMS BIOLOGY RESEARCH GROUPT
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Kravtsov V, Swain M, Schuster A, Du
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Dr Daniel BerrarLecturer in Biomedi
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Zhang et al; Incorporating Feature
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Bala P, Baldridge K, Benfenati E, C
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In addition there is a growing them
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Clinical work involves development
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In 2009 he was appointed Chairman o
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Stevenson TR, Goodall EA and Moore
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Dr Raymond BeirneLecturer in Optome
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Dr Julie McClellandLecturer in Opto
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Graham JE, Moore JE, Moore JE, McCl
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Research Staff:Dr David OrrSenior R
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Dr Victoria McGilliganResearch Asso
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Grant Holder Anderson, Prof RSFundi
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Funding Body Royal Irish AcademyAmo
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14. BIOMEDICAL SCIENCES RESEARCH IN
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Student: Simon GenglerTitle: Effect
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Student: Anisha MazumdarTitle: Anal
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Student: Clare RyanTitle: How does
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CONGRATULATIONS TO THE FOLLOWING PO