2006 - UZ Leuven

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patients with BOS as compared to those without BOS (16 ± 10 Uversus 10 ± 10 U, respectively, p = NS). There was no correlationbetween the anti-PA IgG level and the number of days post LTX, northe FEVI (% of best post LTx) in these patients.Specific serum anti-PA IgG levels in CF patients decline significantlyafter lung transplantation but remain elevated in those patients withchronic colonization of the airways with PA, irrespective of thepresence of BOS.GEUDENS N., VANAUDENAERDE B.M., NEYRINCK A.P., VAN DEWAUWER C., REGA F.R., VERLEDEN G.M., VERBEKEN E., LERUT T.E.,VAN RAEMDONCK D.E.M.: Impact of warm ischemia on differentleukocytes in bronchoalveolar lavage from mouse lung: possible newtargets to condition the polmonary graft from the non-heart-beatingdonor. J. Heart Lung Transplant., <strong>2006</strong>; 25(7): 839-846.Background: The use of non-heart-beating donors (NHBDs) for lungtransplantation is a possible alternative for increasing the number oforgans available. The warm ischemic period after circulatory arrestmay contribute to a higher degree of primary graft dysfunction,resulting from ischemia-reperfusion injury (IRI). A betterunderstanding of the role of inflammatory cells during the warmischemic interval may be useful for developing new therapeuticstrategies against IRI.Methods: Mice were divided in 7 groups (n = 6/group). In 3 groups,ischemia was induced by clamping the hilum of the left lung for 30, 60or 90 minutes (Groups [30I], [60I] or [90I], respectively). In 3 moregroups, the lung was reperfused for 4 hours after identical ischemicintervals (Groups [30I+R], [60I+R] or [90I+R], respectively). Surgicalimpact was evaluated in a sham group ([sham]). Total and differentialcell counts and interleukin-1beta (IL-1beta) protein levels inbronchoalveolar lavage (BAL) were determined and their correlationswere investigated.Results: Total cell, macrophage and lymphocyte numbers and IL-1beta protein levels increased progressively with longer ischemicintervals. A significant rise in BAL macrophages and lymphocytes wasobserved between [60I] and [90I] (p < 0.01 and p < 0.001,respectively). BAL neutrophils only increased after reperfusion withlonger ischemic intervals. A positive correlation was found in theischemic groups between IL-1beta levels and the number ofmacrophages (r = 0.62; p = 0.0012) and the number of lymphocytes (r= 0.68; p = 0.0002). A positive correlation was found in the reperfusiongroups between IL-1beta levels and the number of neutrophils (r =0.48; p = 0.044).100
Conclusions: This study demonstrates for the first time that BALmacrophages and lymphocytes increase significantly during warmischemia and correlate with IL-1beta levels.GEUDENS N., VANAUDENAERDE B., NEYRINCK A., REGA F., VAN DEWAUWER C., VERLEDEN G., VERBEKEN E., VAN RAEMDONCK D.: N-Acetyl cysteine reduces inflammatory triggers in bal from mice lungfollowing warm ischemia and reperfusion. J. Heart Lung Transplant.,<strong>2006</strong>; 25: S 163 (347).Purpose: The warm ischemic period prior to cold preservation of nonheart-beatingdonor (NHBD) pulmonary grafts may increase the risk ofischemia reperfusion injury (IRI). Leukocytes and IL-1β are importanttriggers in the development of IRI. N-Acetyl Cysteine (NAC) is knownto be a potent anti-oxidant. We investigated the impact of NAC oninflammatory events in Broncho-Alveolar Lavage (BAL) in a mousemodel of in situ warm ischemia and reperfusion.Methods: Female SWISS mice were divided in 5 groups (n=6/group).After thoracotomy, the hilum of the left lung was clamped to inducewarm ischemia (I) for 90 minutes in all groups, except in SHAM, andfurther unclamped in 2 study groups for 4 hours of reperfusion (I + R).NAC (10mg/kg, 50µl) or Saline (50µl) were instilled endotracheally 15minutes before the onset of ischemia. After sacrifice, cell count and IL-1β levels were analyzed in BAL from the left lung.Results: (mean ±SD) are listed in Table. There was a positivecorrelation between IL-1β levels and the number of macrophages(r=0,73; p < 0.0001) as well as lymphocytes (r=0.63; p < 0.0001).GroupTotal cells Macrophages Lymphocytes Neutrophils IL-1β(n=6/group) (*10 4 /ml) (*10 4 /ml) (*10 4 /ml)(*10 4 /ml)SHAMSaline+INAC+ISaline + I + RNAC + I + R40.0 ± 7.2195.3 ± 25.1*46.5 ± 7.6*166.0 ±27.764.0 ±22.6 SS 35.7 ± 6.7151.6 ± 20.3*39.3 ±7.1 #119.5 ± 19.953.4 ± 20.0 SS 4.2 ±0.842.6 ± 11.3*7.0 ±1.8 #23.3 ± 7.07.9 ± 1.8 SS 0.4 ± 0.50.9 ±1.40.2 ±0.323.2 ± 9.43.2 ± 3.2 SS 58.6 ± 26.6208.9 ± 37.9*64.4 ± 13.4 #203.2 ± 48.499.0 ±20.5 S* p< 0.001 versus SHAM; # p < 0.001 vs Saline +I; S < 0.01 and SS p < 0.01 vs Saline + I + RConclusion: Administration of NAC attenuated the lymphocyticincrease during ischemia and the rise in neutrophils after reperfusion.Whether there is a biochemical trigger between lymphocytes andneutrophils needs to be further investigated. Preharvest treatment withNAC could be a promising tool to reduce IRI in NHBD lungtransplantation.101
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CYRURGIE2006
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Heelmeesters allerhande, verenig u!
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INHOUDSOPGAVEAbdominale Heelkunde 1
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De resultaten van een grote Noord-A
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VEGF (P = 0.008) correlate with a p
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severe ulcerative ileitis and jejun
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tekens op CT en/of MRI kunnen een b
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data we propose a scoring system in
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ABDOMINALETRANSPLANTATIECHIRURGIECA
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DYCKMANS K., LERUT E., GILLARD P.,
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LERUT J., ORLANDO G., ADAM R., SABB
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histopathologic diagnostic process.
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additional stimulants that the inna
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ARTIKELS UIT HETLEUVENSE NETCREVITS
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PRUYT M., DEVRIENDT D., VANNESTE A.
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BOSHOFF D., BUDTS W., MERTENS L., E
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FLAMENG W., MEURIS B., HERIJGERS P.
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prosthetic valve endocarditis who w
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in these patients We present a case
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SERCA2a. In SKO mice, gene-targeted
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MULTIDISCIPLINAIRBORSTCENTRUMMORALE
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east implant. Only two other cases
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Object: Based on data from primate
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SISCOM hyperperfusion cluster and M
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ONCOLOGISCHEHEELKUNDEBROUNS F., SCH
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infiltrative multilobular spindle c
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multivariable Cox model adjusted fo
Page 58 and 59: We retrospectively evaluated a surg
Page 60 and 61: We performed resection arthroplasty
Page 62 and 63: earing posterior-stabilised. To do
Page 64 and 65: age and key pinch strength. The dif
Page 66 and 67: FABRY K., LAMMENS J., DELHEY P., ST
Page 68 and 69: cases. The mean Knee Society’s kn
Page 70 and 71: short-term solution for his fractur
Page 72 and 73: the foot and result in major septic
Page 75 and 76: SAEGEMAN V., LISMONT D., VERDUYCKT
Page 77 and 78: Objective: The objective of this st
Page 79 and 80: VICTOR J., BELLEMANS J.: Physiologi
Page 81 and 82: skin construct displays authentic f
Page 83 and 84: Bewertung des Spenderdefektes ergab
Page 85 and 86: HIERNER R., BERGER A.: Options and
Page 87 and 88: vascularized ulnar nerve graft and
Page 89 and 90: cranial bone and dura which cannot
Page 91 and 92: defects.Adequate debridement, early
Page 93 and 94: Patients and Methods: Between 1995
Page 95 and 96: MASSAGE P., VANDENHOF B., VRANCKX J
Page 97 and 98: Background: High pressure injuries
Page 99 and 100: VERMEULEN P., DICKENS S., VRANCKX J
Page 101 and 102: inhibitors that neutralize the impa
Page 103 and 104: internship. These components repres
Page 105 and 106: Objective: To evaluate the expressi
Page 107: patients were diagnosed with acute
Page 111 and 112: progressive accumulation of FVIII a
Page 113 and 114: his name to this condition through
Page 115 and 116: gevallen, beschouwen wij de minimaa
Page 117 and 118: Table 1Reperfusion time(min)PVR(dyn
Page 119 and 120: transplantation; dehiscence (n = 25
Page 121 and 122: upon reperfusion results from a red
Page 123 and 124: VAN DE WAUWER C., VAN RAEMDONCK D.E
Page 125 and 126: Bronchiolitis obliterans syndrome (
Page 127 and 128: noodzakelijk een beter inzicht te v
Page 129 and 130: patients of 70 years and older trea
Page 131 and 132: of debate. A good pain relief can b
Page 133 and 134: VAN GESTEL L., NIJS S., BROOS P.: T
Page 135 and 136: UROLOGIEALBERSEN M., JONIAU S., VAN
Page 137 and 138: children achieve bladder and bowel
Page 139 and 140: Results: Ninety-five percent of the
Page 141 and 142: esistant to degradation, but at 365
Page 143 and 144: Results: Although the surgery was m
Page 145 and 146: DE RIDDER D.: Conservatieve aanpak
Page 147 and 148: pressures were measured. The effect
Page 149 and 150: GOEMAN L., JONIAU S., OYEN R., VAN
Page 151 and 152: pelvic lymph node status were not w
Page 153 and 154: literature on nephron-sparing surge
Page 155 and 156: on overall survival was studied. Su
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Material und Methoden: Von 13 Zentr
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a remarkable higher number of forei
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VAN CALSTEREN K., VAN MENSEL K., JO
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VANDE WALLE J.G.J., BOGAERT G.A., M
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Introduction & Objectives: Control
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VAATHEELKUNDEBLADT O., MALEUX G., H
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FOURNEAU I., SABBE T., DAENENS K.,
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computed tomography (CT) and magnet
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We report an unusual case of a uret
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during GPIb stimulation, its activa
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2006 - UZ Leuven

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