Acta Facultatis Ecologiae - Technická univerzita vo Zvolene

29Vicia faba testFor our study the test was modified by the followingmethod:– 2 hours of exposition, evaluation immediatelyafter treatment, ana-telophases;– 2 hours of exposition, evaluation 26 hours aftertreatment (3 hours of pretreatment by 0.05 %colchicine, c-metaphases;– 24 hours of exposition, evaluation immediatelyafter treatment, ana-elophases;– 24 hours of exposition, evaluation 26 hour aftertreatment; c-metaphases.Only the sample with the highest dose of radioactivitywas evaluated and the results are shown inTab. 3.Positive control of Vicia faba seedsTo compare impact of radioactivity from samplescollected around NPP Jaslovské Bohunicewith radiation from the standardized source understandardized conditions we have used in co-operationwith the Faculty of Nuclear Sciences and PhysicalEngineering (the Czech Republic) Gamma-Cell device with emissions of different doses 60 Co.For broader spectra of results a scale of differentlyaged V. faba seeds was used. The results are shownin Tab. 4 and 5.Tradescantia testTaking and evaluation of samples were realizedby the following method:Tab. 3: Results of the Vicia faba testSample No.Activity 137 CspGy/secExpositionin hrsNo. of evaluatedana-telophases *c-metaphasesFrequency of aberrationsin %2 571 2/0 300 0.32 571 2/26 *300 0.32 571 24/0 300 0.62 571 24/26 *300 0.3– 12 hours of exposition, evaluation 20 hoursafter treatment, evaluation of micro-nucleusfrequency per 1000 tetrads;– 12 hours of exposition, evaluation immediatelyafter treatment, evaluation of 500 ana-telophasesI.;– 12 hours of exposition, evaluation 30 hours aftertreatment, evaluation of 500 ana-telophases II.In case of the clone Tradescantia paludosa 02we completed the cytogenetic test in meiosis andthe micronucleus test in the tetrads.The results are shown in Tab. 6.Tab. 4: Results of positive control of Vicia faba seeds (vitality)Age of seed samples inyearsExpositionin Gy/hLength of roots in mm48 hrs 72 hrs 96 hrs22 Blank 3.44 ± 1.48 7.11 ± 2.96 11.67 ± 4.862.5 4.67 ± 1.22 18.55 ± 3.36 24.78 ± 4.735 4.89 ± 2.02 12.00 ± 4.40 15.67 ± 5.6010 6.67 ± 1.37 17.78 ± 3.28 23.89 ± 4.6514 Blank 10.22 ± 1.02 36.22 ± 2.05 48.89 ± 2.192.5 10.56 ± 1.09 27.89 ± 0.87 39.11 ± 1.015 11.44 ± 1.19 30.44 ± 3.13 41.00 ± 4.2210 8.89 ± 0.68 21.55 ± 1.89 29.44 ± 2.632 Blank 13.78 ± 1.50 24.56 ± 3.24 29.22 ± 4.182.5 14.89 ± 0.54 28.44 ± 1.78 38.56 ± 3.565 12.00 ± 1.53 22.67 ± 3.30 32.89 ± 4.6710 14.56 ± 0.87 27.44 ± 1.34 36.89 ± 2.06Control (2-year old) 12.54 ± 0.56 27.09 ± 1.48 36.64 ± 2.86