Acta Facultatis Ecologiae - Technická univerzita vo Zvolene

47RESULTS AND DISCUSSIONFor validation of the process of the new developedcoupling FTC-GFAAS were chosen threedifferent feeding volumes of the sample and completevalidation set were survey for all feeding volumes.The linear range, limit of detection and limitof quantification were appointed, respectively. Finallywere assessed repeatability, reproducibilityand accuracy by analyzing the standard referencematerial.Linear rangesLinear ranges are summarized in Tab. 1. Listedresults show broad ranges, parameters of the calibra-tion curves, coefficients of determination and regressioncoefficients for different feeding volumes.Limit of detection (LOD) and limitof quantification (LOQ)Assessed values for limit of detection and limitof quantification for determination of Sb (III) arelisted in Tab 2.RepeatabilityFig. 3 represent the dependence of measuredconcentration of the Sb(III) against measurementorder (significance level 0,05, selected concentrationwas 20 µg.dm –3 ). Repeatability characterizedby RSD was 1.8 % (n = 20).Tab. 1 Linear ranges, equations of the linear regressions and their coefficients for different feeding volumes Sb(III)Feeding volume[ml]Linear range[µg·dm –3 ]Equation of regression R 21 4,2–800 τ = 1,467+0,268*c 0,99935 1,2–250 τ = 6,184+1,229*c 0,997010 0,4–250 τ = 6,434+2,690*c 0,9958Tab. 2 Summarized values of slopes and increments for assessement of LOD and LOQ over ULA-2 aproach [7]volume [ml] LOD [µg.dm –3 ] LOQ [µg.dm –3 ] Equation of regression1 1,4 4,20 τ = 0,637+0,208*c5 0,39 1,17 τ = 0,480+1,703*c10 0,12 0,36 τ = –0,024+2,948*c20Sb(III) 20g.dm -3 , RSD = 1,8% for (n=20)concentration g.dm -31510501 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20measurementsFig. 3. Repeatability of the determination of the Sb(III) (ρ = 20 µg.dm –3 , feeding vol. 2 ml)