Acta Facultatis Ecologiae - Technická univerzita vo Zvolene

34decapitation and samples of blood, liver and duodenumwere taken in sterile conditions. Hematologicalparameters (the total erythrocyte number– RBC, the hemoglobin content – Hb, the hematocritvalue – PVC, the total number of leucocytes– WBC and the differential blood picture – leucogram)were determined by standard methods [3].For examination of microbiological parametersculture medium for faecal streptococci and the agarmedium No. 2 were used [8]. Parallel to each experimentalgroup, groups of sham-irradiated controlsof equal size were analyzed. The results were statisticallyevaluated by non-pared t-test.RESULTSThe clinical symptoms in irradiated animalsobserved from the 2 nd post-irradiational day were:diarrhea, apathy, somnolence, piloerection and hemorrhageson eyes. The first mortalities occurredon the 3 rd day. Beginning with the 5 th day the animalstook no food and bloody diarrheas were observed.Comparing with controls, they were markedlycachectic. None of irradiated animals survived the10 th day after irradiation. The number of E. coli induodenum increased with time (Tab. 1).In liver the presence of E. coli was observedon the 3 rd and 9 th days after irradiation. In controlsthe livers were sterile. In oral cave, except of thecommon respiratory flora we found in irradiatedanimals E. coli on the 6 th and 9 th days after irradiation.The results of post-iradiational changes ofhematological parameters are given in Tab. 2. Onthe 3 rd day after irradiation both the total erythrocytecount (TEC) and the content of hemoglobinin erythrocytes (EHC) were statistically non–significantlyincreased. The haematocrit value (HT)was statistically significantly increased (P