NASA Scientific and Technical Aerospace Reports

Biological and Physical Space Research Laboratory 2002 Science Review; December 2003, pp. 37; In English; See also
20040073490; No Copyright; Abstract Only; Available from CASI only as part of the entire parent document
The purpose of the Project Scientist Core Facility is to provide purified proteins, both recombinant and natural, to the
Biotechnology Science Team Project Scientists and the NRA-Structural Biology Test Investigators. Having a core facility for
this purpose obviates the need for each scientist to develop the necessary expertise and equipment for molecular biology,
protein expression, and protein purification. Because of this, they are able to focus their energies as well as their funding on
the crystallization and structure determination of their target proteins.
Author
Biotechnology; Molecular Biology; Proteins; Purification; Research Facilities
20040073521 NASA Marshall Space Flight Center, Huntsville, AL, USA
The Building Blocks of Materials: Gathering Knowledge at the Molecular Level
Biological and Physical Space Research Laboratory 2002 Science Review; December 2003, pp. 35; In English; See also
20040073490; No Copyright; Abstract Only; Available from CASI only as part of the entire parent document
Two start-up positions were created within SD46 to pursue developments in the rapidly expanding areas of
biomineralization and nano-technology. As envisioned by Dr. Sandor Lehoczy, the new laboratories to be developed must have
the capacity to investigate not only processes associated with the self-assembly of molecules but also the examination of
self-assembled structures. For these purposes, laboratories capable of performing the intended function, particularly light
scattering spectroscopy and atomic force microscopy were created. What follows then are recent advances arising from the
development of these new laboratories. With the implementation of the Atomic Force Microscopy Facility, examples of
investigations that determine a correlation between the molecular structure of materials and their macroscopic physical
properties are provided. In addition, examples of investigations with particular emphasis on the physical properties of protein
crystals, at the molecular level, and subsequent macroscopic characteristics are as provided. Finally, progress in fabrication
of technology at the nano-scale levels at the developmental stage is also presented.
Author
Calcification; Crystals; Molecular Structure; Molecules; Proteins; Self Assembly
20040073533 North Carolina State Univ., Raleigh, NC
Human Metabolism and Interactions of Deployment- Related Chemicals
Hodgson, Ernest; Aug. 2003; 85 pp.; In English
Contract(s)/Grant(s): DAMD17-00-2-0008
Report No.(s): AD-A419139; No Copyright; Avail: CASI; A05, Hardcopy
This study examines the human-metabolism and metabolic interactions of a subset of deployment-related chemicals,
including chlorpyrifos, DEET, permethrin, pyridostigmine bromide, and sulfur mustard metabolites. Chiorpyrifos is
metabolized by cytochiome P450 (CYP) isoforms with the ratio between activation and detoxication varying dramatically
between different isoforms and polymorphisms. DEET is metabolized by several CYP isoforms while permethrin is first
hydrolyzed then metabolized to phenozybenoic acid by alcohol and aldehyde dehydrogenases. Pyridostigmine bromide is not
readily metabolized by human enzymes preparations. Chiorpyrifos and other organophosphorus compounds have considerable
potential for interactions since they are potent inhibitors of DEET, carbaryl and testosterone metabolism by CYP.
DTIC
Bromides; Deployment; Metabolism
20040073549 Naval Submarine Medical Research Lab., Groton, CT
Stimulus-Frequency Otoacoustic Emissions: Validity and Reliability of SFOAES Implemented on Mimosa Acoustics
SFOAE Measurement System V2.1.18
Lapsley-Miller, Judi A.; Boege, Paul; Marshall, Lynne; Shera, Christopher A.; Jeng, Patricia S.; Feb. 26, 2004; 38 pp.; In
English; Original contains color illustrations
Report No.(s): AD-A422330; NSMRL-TR-1231; No Copyright; Avail: CASI; A03, Hardcopy
The validity and reliability of stimulus-frequency otoacoustic emissions (SFOAEs) were evaluated and determined with
the Mimosa Acoustics SFOAE measurement system (v.2.1.18). Stimulus-frequency otoacoustic emissions are sounds made by
healthy inner ears to tonal stimulation. They are thought to hold the potential for diagnosis and monitoring of noise- induced
hearing loss in military hearing-conservation programs. By establishing the validity and reliability of the measurement,
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