12th Congress of the European Hematology ... - Haematologica

12 th Congress of the European Hematology Association
tested with HemosIL Homocysteine (y=0.8292x+0.3503). Since blood
samples for HemosIL Homocysteine assay are collected in 1/10 (v/v)
sodium citrate, the observed discrepancy could be due to dilution of
blood sample by citrate. After correction for dilution factor, the regression
line between the two measurements was almost superimposable
to the identity line (y=0.9711x + 0.4135). Summary and Conclusions. The
HemosIL Homocysteine assay for tHcy on ACL 9000 compares well
with the established Abbott IMx immunoassay and can be used for routine
measurement of plasma tHcy, especially in subjects who undergo
screening for abnormalities of hemostasis that are associated with
thrombophilic states. As expected, a proportional bias, due to different
plasma dilutions between the EDTA and citrated samples (pendent
0.8292), was corrected applying the appropriate volume correction factor
(pendent 0.9708).
0344
ROLE OF THE PAI-1 GENE 4G/5G POLYMORPHISM ON PAI-1 LEVEL AND RELATIONS
AMONG PAI-1, TNFα AND TGFβ LEVELS IN OBESE CHILDREN
N. Ozbek, S. Tulgar Kinik, B. Atac, M. Yüce, A.C. Yazici, H. Verdi
Baskent University, Faculty of Medicine, ANKARA, Turkey
Objective. Obesity is a metabolic disorder that is associated with
changes of plasminogen activator inhibitor-1 (PAI-1), tumor necrosis factor-alpha
(TNFα) and transforming growth factor beta (TGFβ) levels.
These changes are related to insulin resistance, dyslipidemia and cardiovascular
disease. However the precise effect of the PAI-1 gene 4G allele
is still contradictory. Aims. In this study we aimed to elucidate the role
of the 4G/5G polymorphism of PAI-1 gene on PAI-1 level and determine
the associations between cytokines α (TNFα and TGFβ), glucose
and lipid metabolism parameters in Turkish obese children. Methods.
Thirty nine obese children [12 male and 27 female; mean age of 11.4±3.3
(5.9-17.4) years] and 38 age-matched healthy control group [(19 male and
19 female; mean age of 10.3±3,5 (4.7-17.0) years] were included in the
study. In all cases, serum levels of glucose, lipid and insulin were measured,
homeostasis model assessment of insulin resistance (HOMA-IR)
was calculated, and 4G/5G polymorphism of PAI-1 gene, plasma PAI-1
level and serum TNFα and TGFβ levels were studied. Results. The mean
of relative body mass index (BMI) and HOMA-IR score VLDL, TG,
insulin, PAI-1, TNFα levels were higher and HDL and TGFβ levels were
lower in obese group. The frequency of the PAI-1 gene 4G/4G genotype
was considerably higher in obese children than in controls. There was
a significant interaction between groups and genotypes with regard to
PAI-1 levels in obese children (p