12th Congress of the European Hematology ... - Haematologica

acquire a cytolytic capacity against leukemia cell lines and against allogeneic
primary AML blasts. Summary and conclusions. These results confirm
that NK cells can be significantly expanded under GMP conditions
and indicate that from 100 mL of donor peripheral blood it is possible
to obtain 1-5×10 8 NK cells, which means the opportunity of infusing
into a recipient of 70 kg 1.5-7×10 8 NK cells/kg of body weight. Taken
together, these findings indicate a possible new strategy for the expansion
of cytolytic effectors that may be considered for the management
of AML patients with evidence of disease persistence or recurrence after
an allogeneic SCT. Clinical protocols appear feasible, particularly considering
that the infusion of NK cells should induce very limited toxicity and
no or very low risk of graft-versus-host disease, thus avoiding the potential
complications associated to donor T-lymphocyte infusions.
0594
PLASMA CHANGES OF ENDOTHELIAL INJURY MARKERS IN PATIENTS TREATED WITH
DIFFERENT CONDITIONING REGIMENS FOLLOWED BY HAEMATOPOIETIC STEM CELL
TRANSPLANTATION
A. Czyz, A. Blazejczak, A. Lojko, E. Hanszke, L. Gil, D. Dytfeld,
K. Zawilska, M. Komarnicki
University of Medical Sciences, POZNAN, Poland
Background. The endothelial injury caused by conditioning regimen is
thought to play a central role in the non-infectious complications in
patients undergoing haematopoietic stem cell transplantation. The aim
of the study was to evaluate the plasma changes of endothelial injury
markers in patients treated with different type of preparative regimens
and to investigate whether these changes are associated with toxicity of
conditioning schedule. Patients and Methods. Plasma levels of von Willebrand
factor antigen (vWF Ag), trombomodulin (TM) and vascular
endothelial growth factor (VEGF) were measured by immunoassay tests
in 21 patients (pts), median age 30 (19-67) years, transplanted with allogeneic
(18 pts) or autologous (3 pts) haematopoietic stem cells after
BuCy2 (busulfan 16 mg/kg, cyclophosphamide 120 mg/kg) - 5 pts,
TBI/Cy (total body irradiation 12 Gy, cyclophosphamide 120 mg/kg)- 5
pts, Treo/Cy (treosulfan 14 mg/m2 , cyclophosphamide 120 mg/kg)- 6
pts or fludarabine-based reduced-intensity regimen- 4 pts for AML (10
pts), ALL (5 pts), CML (4 pts) and AA (1 patient). For the statistical analysis
patients were divided into the subgroups according to the type of conditioning
schedule: 1.BuCy2 2.TBI/Cy 3.myeloablative regimens
(BuCy2, TBI/CY) and 4.reduced-toxicity regimens (fludarabine-based
regimen, Treo/CY). Endothelial injury markers were measured before
conditioning regimen (day -10), and on the day of stem cells infusion, 48
hours after finishing preparative schedule, but before stem cells infusion
(day 0). Results. After conditioning regimen vWF Ag concentration
increased significantly on the day 0 in comparison to the day -10 (p<
0.05) in the whole study group. wWF Ag concentration did not differ significantly
between subgroups on the day 0. In the subgroup treated with
TBI based regimen (5 pts) VEGF level increased on the day 0 in comparison
to the day -10 (p