12th Congress of the European Hematology ... - Haematologica

12 th Congress of the European Hematology Association
the pathogenesis and the prognosis of the disease. In our study including
29 patients, we checked CD 38 and CD 138 in order to support the
diagnosis of multiple myeloma. In our study CD87 was negative in 8
patients (27.5%), low possibly positive in 9 patients (31.1%) and bright
positive in 12 patients (41.4%). Extramedullary involvement rate was
meaningfully high in patients with high CD 87 expression. However we
couldn’t find any relationship with other prognostic factors. CD87
expression was meaningfully high in CD45 negative patients. CD45 negativity
and CD 87 (uPAR) positivity correlated with extramedullary
relapse, short lifetime, and bad prognosis in other studies. Flow cytometry
is important in diagnosis and estimating the prognosis of MM. CD
56, CD45 and CD 87 are important for estimating prognosis. However
more prospective studies should be done to understand especially the
effect of CD 87 positivity in prognosis of MM patients.
1523
EFFECT OF GLP-PRO-ARG-GLY ON RAT PLATELET AGGREGATION
M. Grigorjeva, T. Obergan
Moscow State University, MOSCOW, Russian Federation
Background. It has been shown that small glycine-containing peptides
cause an inhibition of blood coagulation, have fibrinolytic and anticoagulant
effects. Besides, various glycine-containing peptides inhibit the
activity of thrombin and platelet aggregation. But mechanisms of peptides
action on haemostasis system, in particular on platelet aggregation,
remain poorly understood. Aims. The aim of the present investigation was
to study the effect of glycine-containing peptide Glp-Pro-Arg-Gly on
ADP-induced platelet aggregation (AIA) and to reveal possible mechanisms
of this action using inhibitor of NMDA- receptors. Methods. The
experiments were carried out on white rats. In vivo experiment AIA were
monitored after intravenous injection of peptide (50 or 200 mkg/kg) or
after peptide on a background of preliminary blockade NMDA-receptors
by amantadine (2 mg/kg). In vitro experiment amantadine was added to
pool rat plasma before ADP (1 mkM). Results. Our experiments on normal
rats demonstrate that intravenous injection of Glp-Pro-Arg-Gly lead
to AIA decrease on 15-28% from preadministration value. Amantadine
effectively blocked the influence of peptide on AIA. On a background of
preliminary blockade NMDA- receptors by amantadine Glp-Pro-Arg-Gly
lost thus effects on platelet aggregation. In this case AIA was increased
as compared with normal rats. In vitro experiment amantadine didn’t
change AIA. Conclusions. Thus we conclude that glycine-containing peptide
Glp-Pro-Arg-Gly inhibits the blood platelet aggregation and NMDAreceptors
participate in realization of peptide anti-platelet activity.
1524
EVALUATION OF NEONATAL SCREENING FOR HEMOGLOBINOPATHIES IN PICARDIE
FROM 1999 TO 2006
V.L. Li Thiao Te, 1 E. Cadet, 2 J.M. Perini, 3 B. Horle, 4 B. Boudailliez, 5
M. Lhermitte, 3 J. Rochette, 2 B. Pautard4 1 CHU Amiens, AMIENS; 2 Génétique Moléculaire, CHU Amiens, AMIENS;
3 Laboratoire de Biochimie, CHRU de Lille, LILLE; 4 Hématologie Pédiatrique,
CHU Amiens, AMIENS; 5 Service de Pédiatrie, CHU Amiens, AMIENS, France
Background. According to the « Association Française pour le Dépistage
et la Prévention des Handicaps de l’Enfant » (AFDPHE), neonatal selective
screening of sickle cell diseases (SCD) and other hemoglobinopathies has
been implemented in metropolitan France since 1999. Related to its proximity
to Ile-de-France, prevalence of SCD is on the rise in Picardie due to
the increasing of people immigration from African countries and French
Antilles. Benefits of early diagnosis is well-demonstrated as far as SCD are
concerned but it also raises some ethical questions regarding detection of
heterozygous babies. Aims. We report the incidence of hemoglobinopathies
and hemoglobinopathy carriers in Picardie over a 8-year period,
from 1999 to 2006. Methods. The targeted screening concerned
neonates whose parents came from high-risk areas. Blood samples were
obtained either from a heel prick or from venous puncture after 72 hours
of life and spotted onto Guthrie cards. Isoelectric focusing was performed
as a primary screening method, completed by high performance liquid
chromatography if necessary. Results. The number of investigated newborns
in Picardie has increased from 738 in 1999 to 4243 in 2006. The overall
incidence of SCD among the 22605 screened neonates was 0,13%
(n=29 of which 21 S/S and 8 S/C) and the frequency of sickle cell trait (A/S)
was 2,35% overall (n=532). The frequency of variant hemoglobin C (HbC)
gene was 0,69% overall including S/C profiles (0,04%), HbC homozygosity
(0,02%) and HbC heterozygosity (0,63%). We found 0,04% of variant
hemoglobin D (HbD) carriers and 0,01% of hemoglobin O Arab het-
540 | haematologica/the hematology journal | 2007; 92(s1)
erozygotes. Parents of all SCD diagnosed neonates received information
about the disease during the first announcement consultation and patients
care is assessed by medical SCD experts. But, less than 1% of heterozygous
newborns parents could be given the information. Discussions. The
increasing number of detected constitutional hemoglobinopathies in
Picardie is related to the increasing number of tested newborns. Although
neonatal screening has resulted in a better follow up of SCD affected children,
the management of hemoglobinopathy carriers neonates needs to
be improved. Recently, we have proposed a specific consultation to disclose
heterozygosity in order to identify high-risk families and try to give
adequate counselling considering the cultural and religious beliefs.
1525
PLATELET ACTIVATION AND ACUTE CORONARY SYNDROME (ACS):
EFFECT OF ANTIPLATELET AGENTS ANALIZED BY FLOW CYTOMETRY
N. Fernandez Mosteirin, C. Salvador Osuna, N. Padron, A. Godoy,
B. Soria, F. Sevil, M. Torres, J.G. Galache-Osuna, J.F. Lucia, M. Giralt
Hospital Universitario Miguel Servet, ZARAGOZA, Spain
Background. Platelet (plt) activity is crucial to the pathogenesis of atherosclerosis
and arterial thrombosis. These are the principal contributors to
the development of ACS. Aim. To study the parameters of platelet activation,
variability of responsiveness to an agonist and response to an
antiplatelet agent such as clopidogrel, in patients with ACS. Patients and
Methods. 26 patients admited to hospital with the diagnosis of ACS undergoling
percutaneous coronary intervention (PCI) were included in the
study. Clinical and therapeutic parameters were evaluated. All patients
had started aspirin intake before the study. Samples were obtained before
PCI. To detect plt response to an agonist (activation) we added a 100 ?M
ADP concentration. The surface expression of plt receptors was determined
by flow cytometry using: anti-CD41 (Gp IIb/IIIa) FITC and PE,
anti-CD62p (P-selectin) PE, anti-CD11b FITC, anti-CD14 PC5
(Immunotech, Marseille, France) and PAC-1 (activated Gp IIb/IIIa) FITC
(BD Biosciences, San Jose, CA, USA).
Table 1.
Control Patients
Median Range Median Range
PAC-1 (MFI) 0.4 0.3 PAC-1 (MFI) 0.4 0.3
0.7 1.10
PAC-1 ADP (MFI) 2.3 0.7 PAC-1 ADP (MFI) 0.95 0.4
5.7 5.50
∆PAC (MFI) 1.8 0.3 ∆PAC (MFI) 0.4 0.1
5.2 4.90
CD62p (%) 11.2 4.4 CD62p (%) 4.95 1.21
18.0 42.5
CD62p ADP (%) 26 11.3 CD62p ADP (%) 1.42 3.34
57.4 68.87
∆CD62p (%) 14 1.7 ∆CD62p (%) 4.87 1.33
44.1 44.3
Plt-Mon (%) 25 9.7 Plt-Mon (%) 20.4 14.15
51.9 40.38
Plt-Mon ADP (%) 50.1 16.4 Plt-Mon ADP (%) 37.61 24.71
96.7 65.38
∆Plt-Mon ADP (%) 23.7 0.8 ∆Plt-Mon ADP (%) 17.01 6.88
68.9 37.67
Plt-Nt ADP (%) 17.3 8.5 Plt-Nt ADP (%) 17.42 6.83
58.5 33.58
The samples were analized on a Coulter ® EPICS XL-MCLTM. PAC-1
was expressed in mean fluorescence units (MFI) of total plt population.
CD62p and leukocytes positive for plts were expressed in percentage
(%). Descriptive statistics and correlation test were also studied. Results
were compared to a control group of 25 healthy donors. Results. Blood
samples from 4 females and 22 males with a median age of 66 years
(range: 44-82) were studied. 11 (42.3%) patients were current smokers,
6 (23.07%) presented hypercholesterolemia, 10 (38.4%) hypertension
and 1 (3,8%) Diabetes mellitus. 9 patients received clopidogrel previous
study were done. See descriptive statistics in Table 1. We found a differences
with statistical significance between controls and patients for
P-selectin with and without agonist (p= 0.032 and p=0.020 respectively)
and for PAC-1 whith agonist (p=0.001). When analized patients with
or without clopidogrel treatment we found statistical significance
between both groups for PAC-1 (p=0.016) and the percentage of neutrophil-platelet
conjugates (p=0.033). Conclusions. a) In our series expression
of P-selectin (alpha granules release) is correlated to the binding of
PAC-1 (conformational change) according to the results of other series,