12th Congress of the European Hematology ... - Haematologica

1069
CLINICAL RELEVANCE OF REGULATORY NETWORK IN THE BONE MARROW
COMPARTMENT OF PATIENTS WITH MULTIPLE MYELOMA
J. Bila, M. Perunicic, I. Elezovic, M. Bakrac, N. Suvajdzic, M. Gotic,
B. Mihaljevic, D. Tomin, A. Vidovic, V. Cemerikic, D. Boskovic
Institute of Hematology, BELGRADE, Serbia
Regulatory network in the bone marrow (BM) compartment of
patients with multiple myeloma (MM) consists of functional interplay
between the myeloma cells and microenvironment resulting with the
interaction of various cytokines, their receptors and adhesion molecules.
The aim of study was to analyze prognostic significance of different
tumor-host interactions in the BM of patients (pts) with MM by
immunohistochemical markers of sensitivity to the IL-6; adhesion molecules;
osteoclastogenesis; and angiogenesis. Patients and Methods. Sixty
newly diagnosed MM pts (33 male/27 female pts, mean age 60 years,
range 35-75) were distributed according to the clinical stage (CS,
Salmon&Durie) as: I 8pts, II 22pts, III 30pts. IgG myeloma was diagnosed
in 35pts; IgA in 12pts; light chains in 12pts. Regarding ISS score,
the group included: ISS1 18pts, ISS2 13pts, ISS3 29pts. All patients were
treated with conventional chemotherapy. All samples of BM biopsies
were analyzed for the immunohistochemical expression of gp 130,
VCAM, OPG and RANKL. The intensity of these staining was graded
as weak (0-30% cells), moderate (31-60% cells), and strong (>60% cells).
Analyzing the microvessel density (MVD), BM vessels were visualized
by immunohistochemical staining for CD34. The number of vessels per
400x high power field (HPF) was counted in the area of the most dense
vascularization. Results. The expression of gp130 was higher in III vs. I
CS (32 vs.15%, p