12th Congress of the European Hematology ... - Haematologica

ly. Conclusions. 1. Combined intensive chemotherapy (C–D, ESHAP or
dexa-BEAM) for PMLBCL allow to achieve complete remission in most
cases. 2. Surgical treatment is effective in local residual diseases eradicating.
3. The radiotherapy should be conducted in purpose of consolidation
of complete remission.
1388
VALUE OF GLYCOSYLATED HEMOGLOBIN (HBA1C) DETECTION ANALYSIS AS SCREENING
METHOD FOR HEMOGLOBINOPATHIES
S. de la Iglesia, J. López, R. Martin, P. Martin, M.T. Gomez Casares,
C.E. Lopez Jorge, N. Navarro, T. Molero
Hospital Negrin, LAS PALMAS DE G.C., Spain
Backround. HbA1c determination is a usual way to control diabetic
patients, because it provides a way to check therapeutic adherence in the
last three months. This analysis is performed by high resolution liquid
chromatography (HPLC), which in some cases shows peaks belonging
to pathologic hemoglobins. Aim. The aim of this study was to review
graphics obtained through HPLC to determine Hb1Ac in diabetic
patients, in order to detect abnormal peaks which would be then crosschecked
by a HPLC hemoglobinopathies specific program, and see if
they belong to known pathologic hemoglobins. Materials and Methods.
Chromatograms belonging to 1500 diabetic patients were checked. HA-
8160 (A. Menarini Diagnostics) analyzer was used for HbA1c detection.
This analyzer uses reversed-phase cation exchange chromatography column,
and dual-wavelength colorimetry (415-500 nm) to carry out its
measurements. Together with HbA1c, this analyzer can also provide the
value for HbA0, HbF, and other abnormal peaks belonging to other fractions.
The software used is not able to correctly separate HbA0 from
HbA2. All samples, extracted in EDTA tubes, that showed abnormal
crests or >2% HbF values, were analyzed by HPLC with specific software
for the detection of β-thalassemias as well as other hemoglobinopathies
(Variant. Bio-Rad. Beta Short Program). Results. From the
1500 analyzed chromatograms, 12 ( 0.8%) showed abnormal peaks,
being then cross-checked by VARIANT detecting; 1 HbD, high HbF in
4 patients, 2 HbS, and 1 HbC. None of these patients showed mycrocytosis
or were previously known by the hematology department at our
hospital. Conclusions. According to our results, it appears interesting to
check chromatograms belonging to diabetic patients, because without
any added cost, structural hemoglobinopathies without mycrocytosis
could be detected. Although the diabetic populations is not the most adequate
because of its median age for the screening of hemoglobinopathies,
it could be very interesting to check pathologic chromatograms from
these patients in order to detect possible previously unknown hemoglobinopathies
in order to perform family studies and genetic counseling
when necessary.
1389
FAILURE OF PUBERTY IN EGYPTIAN β THALASSEMIC PATIENTS: EXPERIENCE IN NORTH
EAST REGION - DAKAHLIA PROVINCE
N. Abdelrazik, 1 H. Ghanem2 1 Mansoura University Children Hospital, MANSOURA, Egypt; 2 Mansoura
Faculty of Medicine, MANSOURA, Egypt
Background. Endocrine complications in thalassaemia major (TM) are
classically considered to be the result of iron deposition in the endocrine
glands. Hypogonatotrophic hypogonadism, which still remains the commonest
endocrinopathy in patients with TM, has been proven to be the
result of hemosiderosis of the gonadotroph cells of the pituitary gland.
The aim of the study. to evaluate the prevalence of delayed puberty and
hypogonadotropic hypogonadism in transfusion-dependent patients
with β-thalassemia major. Patient and Methods. Growth and sexual development
of 40 patients with thalassemia major (20 males, 20 females)
aged 12-22 years were evaluated. The following parameters were measured
in every patient: age, sex, height, weight, Body Mass Index (BMI)
and Tanner’s pubertal staging. For all patients, the following investigations
were done: opthalomogical evaluation, audiograms, skeletal survey,
echocardiography, serum ferritin, liver function tests, hepatitis profile,
serum calcium, phosphorus and blood sugar. Thyroid, parathyroid hormones,
serum follicule stimulating hormone (FSH), luteinising hormone
(LH), testosterone (T), and estradiol (E2) hormone were also measured.
Results. Failure of puberty was present in 80% of boys and 75% of girls
aged 12-22 years. Gonadotropin insufficiency was found in most of the
patients with lack of puberty. Arrested puberty was noted in five boys
(25%) and six girls (30%). Ten girls (50%) did not menstruate, two (10%)
had regular menstrual cycles, one (5%) had irregular menstrual cycles,
12 th Congress of the European Hematology Association
and two (10%) developed secondary amenorrhea. Using univariate
analyses and stepwise logistic regression analysis after adjustment for
confounding factors, serum ferritin at the time of the study was identified
as an independent risk factor for hypogonadotropic hypogonadism,
with an odds ratio of 28.40 (95% confidence interval 3.25-245.15), p=
0.003 with a B value of 3.24 (standard error, 1.12). Conclusions. We conclude
that failure of puberty are very common in our thalassemic patients
which necessitates newer protocols of treatment, correct blood transfusion
and chelation therapy.
1390
THE EFFECTS OF LOSARTAN ON PLATELET AGGREGATION AND HEMATOLOGICAL PARA-
METERS IN PATIENTS WITH NEWLY DIAGNOSED HYPERTENSION: (A CLINICAL STUDY)
I. Yavasoglu, M. Unubol, G. Kadikoylu, Z. Bolaman
Adnan Menderes University, AYDIN, Turkey
Background. Angiotensin II receptor blockers have antiproliferative,
antihypertensive and preventive effects from atherosclerosis. There are
controversial in vitro effects of angiotensin II receptor blockers on platelet
aggregation in several experimental studies. In only one study, losartan
decreased in vivo platelet aggregation with ADP, and ristocetin after 3
weeks. Aim. To evaluate in vivo effects of losartan on platelet aggregation
with ADP, collagen, epinephrine, ristocetin and other hematological
parameters. Methods. Nineteen (13 patients female and 6 male, mean
aged of 55±7 years) with newly diagnosed hypertension were enrolled
to this study. They treated with 100mg/day losartan. Before the treatment
and after 2 months, we analyzed complete blood cells parameters
(Beckman Coulter) and platelet aggregation with collagen, epinephrine,
ristocetin, and ADP (Chrono-log, 570 blood aggregation systems, 2 West
Park Road, Haverton,PA, 19083-4691, USA). The values were analyzed
using two-paired t test. Results. Before the treatment platelet aggregation
with ADP, collagen, epinefrin, ristocetin, were 82±20%, 69±24%,
93±15%, and 80±18.4%, respectively. After 2 months, these rates were
79.8±13%, 62.5±20%, 92±14%, and 63±25%. Although all parameters
decreased, decrease in ristocetin was significant. (p=0.042). The levels of
hemoglobin (before 14±1.6 g/dL, after 13.4±1.3 g/dL) and hematocrit
(before 40.2±5%, after 38.5±3.7%, p0.05). Conclusions Losartan treatment decreased
platelet aggregation with ristocetin and the levels of hemoglobin and
hematocrit. Losartan with these hematological effects may be useful in
the atherosclerosis, inhibiting platelet aggregation and decreasing viscosity,
in addition to antihypertensive effect.
1391
SREENING OF HB S IN THE REGIONAL HOSPITAL OF MALABO (EQUATORIAL GUINEA)
P. Ropero, 1 L. Molina Esteban, 2 F.A. González, 1 M. Polo, 1 M. Mateo, 1
C. Benavente, 1 R. Sánchez-Dominguez, 1 R. Paúl, 1 J. Raso, 2 A. Villegas1 1 Hospital Clínico San Carlos, MADRID, Spain; 2 Control de endemias de
Guinea Ecuatorial, ISCIII, Spain
Background. The sickle cell anemia is one of the genetic diseases more
important in the world, it appears mainly in the black race constituting
in a disease of high prevalence in some countries and originating serious
problems of public health. The greater prevalence of the sickle trait is in
Equatorial Africa in where until 40% of the population are carrying,
being in direct relation with the zones in which falciparum malaria has
been rampant; the disease reaches a prevalence of to 2-3%. Aims. To
determine the incidence of Hb S in the population of Malabo (Equatorial
Guinea). Methods. A total of 328 blood samples of umbilical cord and
placenta were compiled in the regional hospital of Malabo during the
months of February and June of 2005. The conserved samples were gathered
on paper of filter and in methanol to 70% to 4º C. In the Service of
Hematology of the Hospital Clinico San Carlos of Madrid (Spain), the
samples were analyzed in the months of October of 2005 and February
of 2006, being used for the analysis a VARIANT (Bio-Rad) with the program
Sickle Cell Short Program. Results. Of the 328 compiled samples
only 273 could be analyzed of which 208 (76.2%) were normal; 14,2%
(39) displayed the sickle trait (Hb S/Hb A); 8 (2.9%) only have Hb S (Hb
S/Hb S); 18 (6.6%) were carrying of some other hemoglobinopatia non
S (Hb X/Hb A) and 55 samples (16.8%) were degraded not being able to
be studied. Summary/Conclusions. Equatorial Guinea is a country located
in the coast the west of Central Africa in the denominated zone of high
malaria prevalence. It is divided in a continental part on the gulf of
Guinea and another insular one. In the total population it is of 504,000
inhabitants. The capital is Malabo in the island of Bioko. Considering
haematologica/the hematology journal | 2007; 92(s1) | 499