12th Congress of the European Hematology ... - Haematologica

12 th Congress of the European Hematology Association
ed. The white blood cell count (WBC), haematocrit (HCT), platelet number
(PLT) and red blood cell (RBC) count were determined in an automatic
counter (Cell Dyn 900). Results. Analysis of blood cell composition.
WBC count in mice reconstituted with IELC (or BMC) amounted to normal
values after 3 months, platelet number and RBC count-only after 6
months. However HCT and RBC count in mice reconstituted with BMC
were statistically significant reduced in comparison with mice reconstituted
with IELC. The obtained data are likely to be explained by highest
proliferation potential of intestinal cells. Functional activity of immune
system cells in reconstituted mice was studied. The cell-mediated immunity
was estimated according to the degree of manifestation of the
delayed type hypersensitivity reaction (DTHR), namely, the degree of
paw edema upon administration of the resolving dose of T-dependent
antigen (sheep erythrocytes; SE) to sensitized animals. During 3 months
after transplantation, the pronouncedness of DTHR to SE introduction to
mice of both experimental groups was significant reduced in comparison
with the control group. Reconstruction of the cell-mediated immunity in
mice reconstituted with IELC (or BMC) occurred after 6 months. Statistically
significant differences weren’t found between experimental
groups. The humoral immunity was estimated according to the level of
primary humoral response (IgM) to T-dependent antigen (SE). The number
of antibody-forming cells (AFC) in the spleen of experimental mice
was assessed according to the number of local erythrolysis areas in a
semi-liquid medium on day 4 upon immunization. During 3 months
after transplantation, the number of AFC (IgM) in the spleen of experimental
mice was reduced in comparison with the control group. However
the number of AFC in the spleen of mice reconstituted with cells of
intestinal epithelial layer were statistically increased in comparison with
the other experimental group. Reconstruction of the humoral immunity
in mice reconstituted with cells of intestinal epithelial layer (or bone marrow
cells) occurred only after 6 months. Conclusions. Thus, transplantation
of intestinal epithelial layer cells in lethally irradiated mice resulted
in reconstitution of hemato- and immunopoiesis. The obtained data testify
to high hematopoietic potential of intestinal cells.
1451
INFLUENCE OF IRON OVERLOAD ON THE DEATH REASONS AMONG CHILDREN
WITH ACUTE LEUKEMIAS
V. Bebeshko, E. Bruslova, N. Tsvyetkova, L. Lyashenko,
S. Yatzemirskiy, T. Pushkareva
Research Centre for Radiation Medicine, KIEV, Ukraine
Background. At the majority of children during the initial period of
acute leukemia (AL) normochromic normocytic anemias with elevated
serum ferritin level (SF) are revealing. There is a problem of total body
iron overload in these children at the stages of treatment. Aims. To define
SF in dynamics of leukemia process and estimate the patient’s death
reasons with taking into account SF . Methods. At 185 children with AL
(152 - ALL, 33 - AML) SF levels have been investigated according to the
disease period and the patient’s death reason. Children were surveyed
in the Ist (n = 77), in the IInd (n - 35) and in the IIIrd acute periods (n =
5), in the Ist (n =54) and in the IInd remissions (n = 14). The control group
included 30 healthy children. The SF were defined by radioimmune
analysis. Result. In the Ist acute period the SF was 329,1±19,9 ng/L, during
the IIIrd acute period - 1365,7±190,9 ng/L, in the Ist remission -
226,7±30,5 ng/L, in IInd remission - 724,2±76,6 ng/L. At 10/19 patients
with AML and 23/58 children with ALL in Ist acute period SF were >500
ng/mL. At children of comparison group mean SF was 42,4±11,8 ng/L.
From 72 patients, who have died, 37/72 patients had SF >500 ng/mL
(mean 872,2±29,4 ng/mL); in 35/72 patients SF were