Timing, hosts and locations of (grouped) events of NanoImpactNet

NanoSafetyCluster - Compendium 2012
identity. The capacity to quantitatively track the particles over
long periods of time allows us to determine their biological fate,
opening the way for significant advances in our understanding
of the transport pathways used by nanoparticles to access the
brain, using advanced quantitative dosimetrics, selected and
controlled exposure scenarios, long-lifetime radiolabelled
nanoparticles, and biophysical approaches such as fluorescently
labelling certain proteins involved in the transport pathways and
determining co-localization of the proteins and nanoparticles.
• deliver the basis for an inventory of nanoparticles
based on potential for exposure, categorising nanoparticles on
the basis of physicochemical, structural and toxicological
properties:
As above, the capacity to categorise nanoparticles based on
their biological identity will offer key advances in our predictive
skills, and enable us to connect high throughput screening data
to a screening predictive phenomenological models.
• establish relations between experimental (based on
available data) and computational properties:
6 References
1. Cedervall, T., Lynch, I., Lindman, S., Nilsson, H., Thulin, E.,
Linse, S., Dawson K.A. PNAS 2007, 104, 2050-2055.
2. Cedervall, T., Lynch, I., Foy, M., Berggård, T., Donnelly, S.C.,
Cagney, G., Linse, S., Dawson, K.A. . Angew. Chem. Int. Ed. 2007,
46, 5754 -5756.
3. Lundqvist, M., Stigler, J., Cedervall, T., Elia, G., Lynch, I.,
Dawson, K.A. PNAS 2008, 105, 14265-14270.
7 Directory
Table 1 Directory of people involved in the NanoTransKinetics project as beneficiaries*.
Data from several successful EU FP6 and FP7 projects is being
used as the basis for the project (the minimum set of
experimental data is already under our control via the
NanoInteract, NeuroNano and BioNanoInteract projects which
were coordinated by P1, and additional data will be acquired,
assessed and implemented via WP5, the NanoSafety cluster and
the twinned US projects). As the modelling approaches
described are phenomenological initially, they rely directly on
the experimental data, in order to reproduce the phenomena,
such as protein corona formation, nanoparticles uptake and subcellular
transport and localisation) and we see a key outcome of
the project as being a set of modelling tools that can be linked
directly to High Content Analysis assays, such as lysosomal load,
in order to correlate impacts with sub-cellular localisation and
bioaccumulation potential, for example. We believe that the
approach outlined here is the only realistic possibility for
understanding and predicting the implications of nanoparticles
for living systems, as it is based on understanding the
mechanisms of interaction of nanoparticles with cells.
4. Walczyk, D., Baldelli-Bombelli, F., Campbell, A., Lynch, I.,
Dawson, K.A. J. Am. Chem. Soc. 2010, 132, 5761-5768.
5. Salvati, A.; dos Santos, T.; Varela, J.; Pinto, P.; Lynch, I.;
Dawson, K.A., NanoMedicine, 2011, in press.
6. Shapero, K., Fenaroli, F., Lynch, I., Cottell, D.C., Salvati, A.,
Dawson, K.A. Time and space resolved uptake study of silica
nanoparticles by human cells. Mol Biosyst. 2010 Sep 29. [Epub
ahead of print]
7. Watson, P. J., A.T., Stephens, D.J. Adv. Drug Del. Rev. 2005,
57, 43- 61.
First Name Last Name Affiliation Address e-mail
Vicki Colvin Rice University Houston
TX 77005-1827
USA
Kenneth Dawson National University of Ireland, Dublin /
University College Dublin
Belfield
IE-4 Dublin
Ireland
Giancarlo Franzese University of Barcelona Av. Diagonal 647
Barcelona 08028
Spain
Iseult Lynch National University of Ireland, Dublin /
University College Dublin
Aine Moore National University of Ireland, Dublin /
University College Dublin
Belfield
IE-4 Dublin
Ireland
Belfield
IE-4 Dublin
Ireland
Joachim Rädler Ludwig-Maximilians University Geschwister-Scholl-
Platz 1
Munich 80539
Germany
colvin@rice.edu
kenneth.a.dawson@cbni.ucd.ie
gfranzese@ub.edu
iseult.lynch@cbni.ucd.ie
aine.moore@cbni.ucd.ie
joachim.raedler@lmu.de
202 Compendium of Projects in the European NanoSafety Cluster