LCS III - Konica Minolta Sensing Americas, Inc.

Standard Operations
5.7.1.2 Take single wavelength measurements (single reading)
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1. Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press Zero.
Note:The Read key is only active after the zero measurement has
been completed.
2. Insert the sample cuvette/cell into the cuvette/sample cell
holder. Press Read.
3. For data storage, see section 5.3.1 on page 28.
5.7.1.3 Take single wavelength measurements (continuous readings)
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press Zero.
Note: In the reading mode ”Continuous“ only the Zero key is shown to
start the reading. The reading sequence is started automatically.
2. Insert the sample cuvette/cell into the cuvette/sample cell
holder.
3. Press Options and then the Store icon to store the displayed
data in the Data Log.
Note: For data storage, see section 5.3.1 on page 28.
5.7.2 Multi-Wavelength mode – measurements at more than one wavelength
5.7.2.1 Set the reading mode at different wavelengths
In the multi-wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
Absorbance measures the amount of light absorbed by the sample,
in units of Absorbance.
% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.
Press Multi Wavelength in the Main Menu. Press Options for
Parameter Setup. Table 8 on page 59 describes the multi
wavelength setup options.