Identifikation und funktionale Charakterisierung - Logo Dragon-Ivf ...

Zusammenfassung
recombinant OR expression and Ca 2+ -imaging, for the first time, endogenous ligands were
matched to specific sperm ORs (OR7A5-Furaneol, OR4D1-5α-Androst-16-en, OR1D2-
Buttersäure and 1-Octen-3-on).
In parallel I have examined the electrophysiological properties of immature male germ cells
of mice. This project is focussed on cultured mouse spermatogonia, since the physiology of
these cells is almost unknown. I could establish a coculture system of spermatogonia and
Sertoli cells and I identified voltage-activated K + and Ca 2+ channels. Furthermore, I found
different P2X receptors in spermatogonia and I was able to provide data suggesting the
expression of TRP channels in murine spermatogonia.
Using a combination of molecular and cellular methods as well as high-resolution Ca 2+ -
imaging and electrophysiological recording techniques, the present data provides new insights
into the role of mammalian ORs in the context of maturation of germ cells and physiological
properties of mammalian germ cells. In addition, this work represents the first
electrophysiological description of cultured mouse spermatogonia.
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