Identifikation und funktionale Charakterisierung - Logo Dragon-Ivf ...

2.5 Bakterienstämme
Material & Methoden
Der Bakterienstamm E. coli XL1-Blue (Genotyp: recA1, endA1, gyrA96, thi-1, hsdR17,
supE44, relA1, lac. ,F‘[proAB, lacIqZM15, Tn10, (Tetr)]), stammt aus der Sammlung des
Lehrstuhls für Zellphysiologie der Ruhr-Universität.
2.6 Oligonukleotide
OR-Rezeptoren
Primer Sequenz
HTPCR 16 up 5’- TCA ATC CTG ACT GTC CAA GGA AAA TGA TTG-3’
HTPCR 16 down 5’- AAA GAC TTT CCT CCA TTT TTC ATG TGG-3’
HT-2 up 5’- TCC ACC ATG GCC TAT TTT CCA ATT CTC-3’
HT-2 down 5’- AGG CCT AGG TGT TAA ATT TTG CTG CC-3’
HGMP07J up 5’- TCC ACC ATG GTT TTA TGT TTC AGA TTT GGG-3’
HGMP07J down 5’- ATG TTG ACA GCC TCA GGA GAA CTC TTC-3’
HGMP07I up 5’- TCC ACC ATG ATG GGA CAA AAT CAA ACC AGL-3’
HGMP07I down 5’- ACT GGA TAA ACT ATG TAA TAG CTC CAA GTG-3’
HGMP07E up 5’- TCC ACC ATG GAT GGA GGC AAC CAG AGT GAA GG-3’
HGMP07E down 5’- ATA GTC TCC ACT TTA ATG CTG TCT TTC C-3’
HTPCR 86 up 5’- TCC ACC ATG GAA CCA GGA AAT GAT ACA CAA ATT TC-3’
HTPCR 86 down 5’- ATG ACA GTT ACT ACC TCT GAA GCT TAG AGC-3’
17-40- CT up-nar 1 5’- GTC GGC GCC TAC AGC TTC AGA AAC CCT GAT GTG C-3’
17-40-Cla 1-3’ 5’- GCC ATC GAT AGC CAG TGA CCG CCT CCC TG-3’
17-209 3-Primer 5’-TAC ATC ACT GAT GCA ACT GTG TCC-3’
17-209 5-Primer 5’-GCA GAT GCC – GC TTT GTG TCC ACC-3’
17-210 3-Primer 5’-TAC ATC ACA GCC ATG ACA GTG TCC-3’
17-210 5-Primer 5’-TCT GAC CTC TGC TTT TCC TCG GTC-3’
ATP Rezeptoren (P2X)
Primer Sequenz
P2X1 Forward 5’- ATG CTA GCC TGC CCT TGA CC -3’
P2X1 Reverse 5’- CCT GGG GTC CCA GGC CCT GG -3’
P2X2 Forward 5’- ATG GCC GCT GCA CAG CCC CG -3’
P2X2 Reverse 5’- CTG GTG ATG ATG CTG ACT AC -3’
P2X3 Forward 5’- ATG AGC TAA CTC AAG AGT GG -3’
P2X3 Reverse 5’- GGG GCT TGA GGA AAA GAG CC -3’
P2X4 Forward 5’- ATG GCA GGC TGC TGC TCC G -3’
P2X4 Reverse 5’- CCT GCG TCT GGT TCA CGG TG -3’
P2X5 Forward 5’- ATG GGC CAG GCG GCC TGG AA -3’
P2X5 Reverse 5’- CCT GCC GCT GGT TAG GAG TC -3’
P2X6 Forward 5’- ATG CAG TTG CAG CCA GCA GG -3’
P2X6 Reverse 5’- GAA CAC GTT CTC TCC CTG TG -3’
P2X7 Forward 5’- ATG CCG GCT TGC TGC AGC TG -3’
P2X7 Reverse 5’- CAT GAC AAA GAA TGA GTT CC -3’
36