Handbook of Size Exclusion Chromatography and Related ...

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18. JM Goldwasser. Proc Int Gel Permation Chromatography Symposium 1989, Newton, MA, 1990, p 150. 19. JJ Kirkland, SW Rementer, WW Yau. J Appl Polym Sci 48:39, 1991. 20. WW Yau. Chemtracts-Macromol Chem 1:1, 1990. 21. PJ Flory, TG Fox, Jr. J Am Chem Soc 73:1904, 1951. 22. OB Ptitsyn, YE Eizner. Sov Phys Tech Phys 4:1020, 1960. 23. HG Barth, WW Yau. Proc Int Gel Permation Chromatography Symp 1989, Newton, MA, 1990, p 26. 24. MB Huglin, ed. Light Scattering from Polymer Solutions. New York: Academic Press, 1972, Ch 6. 25. MB Huglin. In: J Brandrup, EH Immergut, eds. Polymer Handbook, 3rd ed. New York: John Wiley and Sons, 1989. 26. H Eisenberg. Biological Macromolecules and Polyelectrolytes in Solution. Oxford: Clarendon Press, 1987. 27. H Utiyama. In: MB Huglin, ed. Light Scattering from Polymer Solutions. New York: Academic Press, 1989, Ch 4. 28. M Martin. Chromatographia 15:426, 1982. 29. O Prochazka, P Kratochvil. J Appl Polym Sci 3:919, 1986. 30. O Prochazka, P Kratochvil. J Appl Polym Sci 34:2325, 1987. 31. R Bruessau. In: J Cazes, ed. Liquid Chromatography of Polymers and Related Material—II. New York: Marcel Dekker, 1983, p 73. 32. D Lecacheux, J Lesec. J Liq Chromatogr 5:2227, 1982. 33. TH Mourey, SM Miller. J Liq Chromatogr 13:693, 1990. 34. C-Y Kuo, T Provder, ME Koehler, AF Kah. ACS Symp Ser 352:130, 1987. 35. ST Balke, P Cheung, L Jeng, R Lew. JAppl Polym Sci, Appl Polym Symp 48:259, 1991. 36. TH Mourey, SM Miller, ST Balke. J Liq Chromatogr 13:435, 1990. 37. C Jackson, WW Yau. J Chromatogr 645:209, 1993. 38. Z He, X Zhang, R Cheng. J Liq Chromatogr 5:1209, 1982. 39. K Lederer, G Imrich-Scwarz, M Dunky. J Appl Polym Sci 32:4751, 1986. 40. J Billiani, DK Lederer, M Dunky. Angew Makromol Chem 180:199, 1990. 41. J Billiani, I Amtmann, T Mayr, K Lederer. J Liq Chromatogr 13:2973, 1990. 42. J Billiani, DK Lederer. J Liq Chromatogr 13:3013, 1990. 43. AE Hamielec. In: J Janca, ed. Steric Exclusion Liquid Chromatography of Polymers. Chromatographic Science Series, Vol. 25. New York: Dekker, 1984, Ch 3. 44. MA Haney, JE Armonas, L Rosen. ACS Symp Ser 352:119, 1987. 45. MG Styring, JE Armonas, AE Hamielec. ACS Symp Ser 352:104, 1987. 46. FB Malihi, CY Kuo, ME Kochler, T Provder, AF Kah. Org Coat Appl Polym Sci Proc 48:760, 1983. 47. FB Malihi, CY Kuo, ME Koehler, T Provder, AF Kah. ACS Symp Ser 245:281, 1984. 48. S Pang, A Rudin. Polymer 33:1949, 1992. 49. DJ Nagy. Int GPC Symp ’87, 1987, p 250. 50. DJ Nagy, DA Terwilliger. J Liq Chromatogr 12:1431, 1989. 51. DJ Nagy. J Liq Chromatogr 13:677, 1990. 52. G Marot, J Lesec. Int GPC Symp ’87, 1987, p 113. © 2004 by Marcel Dekker, Inc.
53. G Marot, J Lesec. J Liq Chromatogr 11:3305, 1988. 54. T Housaki, K Sato. Polym J (Tokyo) 20:1163, 1988. 55. D Lecacheux, J Lesec, C Quivoron, R Prechner, R Panaras. J Appl Polym Sci 29:1569, 1984. 56. ME Himmel, K Tatsumoto, KK Oh, DK Johnson, HL Chum. ACS Symp Ser 397:82, 1989. 57. ME Himmel, K Tatsumoto, K Grohmann, DK Johnson, HL Chum. J Liq Chromatogr 498:93, 1990. 58. EJ Siochi, MA Haney, W Mahn, TC Ward. ACS Symp Ser 397:100, 1989. 59. JD Timpa. J Agric Food Chem 39:270, 1991. 60. BP Wasserman, JD Timpa. Starch/Staerke 43:389, 1991. 61. ML Fishman, DT Gillespie, SM Sondey, RA Bardord. J Agric Food Chem 37:584, 1989. 62. ML Fishman, DT Gillespie, SM Sondey. Carbohydr Res 215:91, 1991. 63. PK Dutta. J Chromatogr Sci 28:119, 1990. 64. PK Dutta, K Hammons, B Willeby, MA Haney. J Chromatogr 536:113, 1991. 65. HE Adams. In: KH Altgelt, L Segal, eds. Gel Permeation Chromatography. New York: Dekker, 1971, p 391. 66. Z Grubisic-Gallot, JP Lingelser, Y Gallot. Polym Bull (Berlin) 23:389, 1990. 67. BH Zimm, WH Stockmayer. J Chem Phys 17:1301, 1949. 68. J Roovers. In: Encyclopedia of Polymer Science and Technology, Vol. 2, 2nd ed. New York: J. Wiley and Sons, 1989, p 478. 69. EJ Siochi, JM DeSimone, AM Hellstern, JE McGrath, TC Ward. Polym Prep (Am Chem Soc Div Polym Chem) 30(1):141, 1989. 70. EJ Siochi, JM DeSimone, AM Hellstern, JE McGrath, TC Ward. Macromolecules 23:4696, 1990. 71. C Kuo, T Provder, ME Koehler. Polym Mater Sci Eng 65:142, 1991. 72. FM Mirabella, Jr, L Wild. Polym Mater Sci Eng 59:7, 1988. 73. DG Moldovan. Int GPC Symp ’87, 1987, p 129. 74. D Lecacheaux, J Lesec, C Quivoron. J Appl Polym Sci 27:4867, 1982. 75. D Lecacheux, J Lesec, C Quivoron. Polym Prepr (Am Chem Soc, Div Polym Chem) 23(2):126, 1982. 76. PJ Wang, RJ Rivard. J Liq Chromatogr 10:3059, 1987. 77. C Jin, S Sun, S Sui. Yingyong Huaxe 3:50, 1986. 78. C Jin, S Sun, R Hou, R Cheng. Gaofenzi Xuebao 2:113, 1987. 79. BE Boyes, HG Barth, WW Yau. 200th ACS National Mtg., Washington, D.C., August 1990, Dn. Agr. Food Chem., Abstract 147. 80. KE van Holde. Physical Biochemistry. 2nd ed. Englewood Cliffs, NJ: Prentice-Hall, 1985. 81. W Zhang, M Wang, J Sun, X Zhang, Z He. Yingyong Huaxue 7(3):57, 1990. 82. F Schosseler, H Benoit, Z Grubisic-Gallot, C Strazielle, L Leibler. Macromolecules 22:400, 1989. 83. WG Rand, AK Mukherji. J Appl Polym Sci. Polym Lett Ed 20:501, 1982. 84. A Lapp, C Strazielle. Makromol Chem 184(9):1877, 1984. © 2004 by Marcel Dekker, Inc.
Page 1 and 2:
MARCEL DEKKER Handbook of Size Excl
Page 3 and 4:
CHROMATOGRAPHIC SCIENCE SERIES A Se
Page 5 and 6:
60. Modern Chromatographic Analysis
Page 7 and 8:
Preface to the Second Edition Gel p
Page 9 and 10:
Preface to the First Edition Molecu
Page 11 and 12:
Contents Preface to the Second Edit
Page 13 and 14:
17. Size Exclusion Chromatography o
Page 15 and 16:
James F. Curry Analytical Departmen
Page 17 and 18:
Iwao Teraoka, Ph.D. Othmer Departme
Page 19 and 20:
The graphical data display typicall
Page 21 and 22:
polymer molecule can elute. The tot
Page 23 and 24:
individual states) allowed to them.
Page 25 and 26:
unsaturation while the IR detector
Page 27 and 28:
Sampleconcentrationshouldbeminimize
Page 29 and 30:
averagesdefinedintermsofthemolecula
Page 31 and 32:
information regarding appropriate M
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Analytical. Two models, the KMX-6 a
Page 35 and 36:
Figure 6 Overlay of time-sliced pea
Page 37 and 38:
accurately for very large and very
Page 39 and 40:
4 GENERAL REFERENCES The interested
Page 41 and 42:
46. TA Chamberlin, HE Tuinstra. US
Page 43 and 44:
materials are commercially availabl
Page 45 and 46:
Table 1 Commercial Column Packing M
Page 47 and 48:
necessarily comparable. For this re
Page 49 and 50:
adjusted such that the pore size di
Page 51 and 52:
narrowmolecular weight range, indiv
Page 53 and 54:
Figure 8 Effect of particle size on
Page 55 and 56:
Figure 9 SEC calibration using poly
Page 57 and 58:
Table 4 Typical Eluent Systems for
Page 59 and 60:
Figure 12 Analysis of poly-2-vinyl
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24. E Meehan, S O’Donohue. The ro
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into accepted laboratory techniques
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Table 1 Trace Metal Impurities in C
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mass and biological activity, for m
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Experimental efficiency vs. velocit
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Table 3 Properties of SEC Silica Ge
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operate at aflow rate that can easi
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Figure 3 Efficiency of amide-bonded
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mechanically stable packing materia
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Figure 5 Pore size distributions of
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Figure 6 Separation of polystyrenes
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60-120 A ˚ are large enough to be
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Table 6 Selected Silica-Based Colum
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Table 7 Separation Ranges for Polym
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the decline in permeability is perm
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sample components can be varied by
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Figure 10 Diol bonding reactions. I
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ecause of the higher relative molec
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valuesfork1,k2,anda,theunknownmolec
Page 99 and 100: water-soluble and detergent-soluble
Page 101 and 102: the importance of secondary retenti
Page 103 and 104: discussed in Table 8(33). Based on
Page 105 and 106: individual dispersion processes ins
Page 107 and 108: The detector time constant can dist
Page 109 and 110: 5.3 Mobile Phase A mobile phase is
Page 111 and 112: less steep and still linear at high
Page 113 and 114: 35. P Bristow, J Knox. Chromatograp
Page 115 and 116: 112. K Gooding, K Lu, G Vanecek, F
Page 117 and 118: for branched polymers or copolymers
Page 119 and 120: Figure 1 “Bridge design” flow-r
Page 121 and 122: Figure 3 A light-scattering photome
Page 123 and 124: Figure 5 SEC universal calibration
Page 125 and 126: where hn is the hydrodynamic volume
Page 127 and 128: It should be noted that dn=dc also
Page 129 and 130: sensitivity for many samples compar
Page 131 and 132: that is, log[h] vs. logM,generated
Page 133 and 134: Figure 8 Effect of band broadening
Page 135 and 136: Table 1 Measurement of Molecular We
Page 137 and 138: Figure 10 Differential refractomete
Page 139 and 140: Figure 11 (A) Mark-Houwink plot of
Page 141 and 142: Table 3 Measurement of Molecular We
Page 143 and 144: size information is derived from un
Page 145 and 146: Table 5 Molecular Weight Distributi
Page 147 and 148: By measuring the scattered intensit
Page 149: 8 APPENDIX: INSTRUMENT COMPANIES 8.
Page 153 and 154: 125. D Slootmaekers, JAPP Van Dijk,
Page 155 and 156: 200. JG Bindels, GJJ Bessems, BM De
Page 157 and 158: possibly with hydrodynamic volume (
Page 159 and 160: An insurmountable limitation of SEC
Page 161 and 162: to sDR(V) when either nPS ¼nPB ¼c
Page 163 and 164: 1,4-trans;and8%1,2-vynil.TheglobalC
Page 165 and 166: The goal is to find the MWD and CCD
Page 167 and 168: As expected, pS is closer to the no
Page 169 and 170: Figure 3 Example 3: MWD and BD of a
Page 171 and 172: 9. BH Zimm, WH Stockmayer. The dime
Page 173 and 174: 45. RO Bielsa, GR Meira. Linear cop
Page 175 and 176: solubility properties. They are use
Page 177 and 178: determination of the absolute molec
Page 179 and 180: Figure 4 Result of the PBT analysis
Page 181 and 182: Figure 8 Result of natural spider s
Page 183 and 184: 7 Size Exclusion Chromatography of
Page 185 and 186: As pointed out earlier, rubber must
Page 187 and 188: Table 2 Various Solvents and Operat
Page 189 and 190: Figure 2 Typical GPC calibrations w
Page 191 and 192: © 2004 by Marcel Dekker, Inc. Figu
Page 193 and 194: Natural and Synthetic Rubbe177 vari
Page 195 and 196: Another example, shown in Fig. 5 (3
Page 197 and 198: APPENDIX: SEC CONDITIONS FOR RUBBER
Page 199 and 200: Appendix (Continued) Polymer Column
Page 201 and 202:
Appendix (Continued) Polymer Column
Page 203 and 204:
Page 205 and 206:
5. J West, E Rodriguez. Rubber Chem
Page 207 and 208:
8 Size Exclusion Chromatography of
Page 209 and 210:
associate. Girdler (67) and Speight
Page 211 and 212:
Table 1 Fractions Obtained Using Co
Page 213 and 214:
Figure 1 SEC analyses of an aged as
Page 215 and 216:
Apparently more polar compounds are
Page 217 and 218:
40% was fractionated into four frac
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Figure 7 SEC analyses of an unaged
Page 221 and 222:
Figure 9 Comparison of percentage L
Page 223 and 224:
Figure 11 Comparison of SEC chromat
Page 225 and 226:
Figure 12 Comparisons of SEC chroma
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percentage LMS at the other locatio
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the total area into up to 12 sectio
Page 231 and 232:
attempted to develop an SEC method
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parameterisbasedontheinternalpressu
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5 MODIFIED ASPHALTS The addition of
Page 237 and 238:
Oxidation of the rubber and asphalt
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Figure 20 SEC chromatogram for an a
Page 241 and 242:
Figure 21 Comparisons of apparent m
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© 2004 by Marcel Dekker, Inc. S/
Page 245 and 246:
PS/10 4 þ 10 3 þ 500 þ 100 THF/1
Page 247 and 248:
Nevertheless, SEC of asphalts is es
Page 249 and 250:
56. NW Garrick, RR Biskur. Trans Re
Page 251 and 252:
Asphalts 235 122. M-S Lin, JM Chaff
Page 253 and 254:
Page 255 and 256:
Table 1 Applications of Acrylamide
Page 257 and 258:
(AM/AA), and acrylamide/dimethyldia
Page 259 and 260:
polymer, a large size filter of 5,
Page 261 and 262:
Figure 1 Size exclusion chromatogra
Page 263 and 264:
Table3 MWandMWDofaBroad-MWDPAMStand
Page 265 and 266:
Another commercially available MW d
Page 267 and 268:
Table 5 Weight-Average Molecular We
Page 269 and 270:
Figure 6 (a) Size exclusion chromat
Page 271 and 272:
compared to anormal product. By com
Page 273 and 274:
StudyingtheKineticsofaChemicalReact
Page 275 and 276:
this information, the higher MW pea
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
46. CJ Kim, A Hamielec, A Bendek. J
Page 283 and 284:
Page 285 and 286:
A relatively new technique utilizin
Page 287 and 288:
where ais the exponent of the Mark-
Page 289 and 290:
Figure 2 TDS chromatograms for full
Page 291 and 292:
Figure 5 Comparison of molecular we
Page 293 and 294:
Table 3 Summary of TDS Molecular We
Page 295 and 296:
thepartiallyhydrolyzedPVAwiththecol
Page 297 and 298:
Table 5 Summary of Conformation and
Page 299 and 300:
a¼0.627andlog(K) ¼23.249.Theseare
Page 301 and 302:
The limits of detection of the PVA
Page 303 and 304:
Figure 12 PVAc broad molecular weig
Page 305 and 306:
4 SUMMARY Advances in SEC character
Page 307 and 308:
Page 309 and 310:
2.1 Molecular Weight Grades of PVP
Page 311 and 312:
exclusion chromatography with low-a
Page 313 and 314:
weightandmolecularweightdistributio
Page 315 and 316:
© 2004 by Marcel Dekker, Inc. Figu
Page 317 and 318:
Figure 4 PEOcalibrationofUltrahydro
Page 319 and 320:
Figure 6 Overlay of GPC chromatogra
Page 321 and 322:
plus Ultrahydrogel 120 A ˚ ,Shodex
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Figure 10 SEC traces of PVP/AA copo
Page 325 and 326:
Figure 11 Molecular weight distribu
Page 327 and 328:
Page 329 and 330:
2 CHEMICAL, MACROMOLECULAR AND MORP
Page 331 and 332:
Table 2 The Relative Composition of
Page 333 and 334:
considered to be 3. After the DS ha
Page 335 and 336:
een extensively studied by methods
Page 337 and 338:
The silylation was performed in LiC
Page 339 and 340:
observed using column materials hav
Page 341 and 342:
Table 6 (Continued) Packing materia
Page 343 and 344:
4.5 Other Cellulose Derivatives Bes
Page 345 and 346:
Table 8 SEC Conditions for Characte
Page 347 and 348:
dextrans swell too much in cadoxen
Page 349 and 350:
stirring at room temperature for an
Page 351 and 352:
Figure 2 MMDofunbleached(HP)andblea
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Figure 4 MMDofbirchkraftpulpdegrade
Page 355 and 356:
adequate when evaluating the influe
Page 357 and 358:
differential refractive index (DRI)
Page 359 and 360:
30. HA Krässig. Effects of structu
Page 361 and 362:
67. E Sjöholm, K Gustafsson, A Col
Page 363 and 364:
104. B Fleury, J Dubois, C Léonard
Page 365 and 366:
136. D Miller, D Senior, R Sutcliff
Page 367 and 368:
166. SS Cutié, CG Smith. Determina
Page 369 and 370:
200. R Berggren, F Berthold, E Sjö
Page 371 and 372:
13 Molar Mass and Size Distribution
Page 373 and 374:
measurements, and vapor pressure os
Page 375 and 376:
The calibration of SEC columns by c
Page 377 and 378:
The division according to eluent ty
Page 379 and 380:
Apolymer produced by UV irradiation
Page 381 and 382:
Because DMAC/LiCl is also agood sol
Page 383 and 384:
compoundsandwasexplainedbyadsorptio
Page 385 and 386:
isolated lignins because they are e
Page 387 and 388:
The Separon HEMA and Separon HEMA B
Page 389 and 390:
Figure 4 GPC elution curves of the
Page 391 and 392:
liquor is highest throughout the co
Page 393 and 394:
topological anisotropy in the polym
Page 395 and 396:
29. M Ristolainen, R Alen, J Knuuti
Page 397 and 398:
eds. Proc Int Symp 1998. Canton, Pe
Page 399 and 400:
92. L Jurasek. Towards a three dime
Page 401 and 402:
Table 1 Selected Industries Connect
Page 403 and 404:
into C3-metabolites, which then may
Page 405 and 406:
Table 4 Key Enzymes in the Biosynth
Page 407 and 408:
Table 5 Cloned Mutants of Starch En
Page 409 and 410:
Figure 3 Modeled x-ray diffraction
Page 411 and 412:
In the native environment (plant ce
Page 413 and 414:
Figure 6 (a) Large starch granules
Page 415 and 416:
number of branching points); crossl
Page 417 and 418:
Both absolute approaches are extrao
Page 419 and 420:
Table 7 (Continued) Approach Experi
Page 421 and 422:
structures, excluded volumes, and t
Page 423 and 424:
Figure 10 (a) SEC elution profile o
Page 425 and 426:
Figure 12 Wheat glucans: DRI elutio
Page 427 and 428:
Figure 13 Wheat glucans. Normalized
Page 429 and 430:
Figure 16 Wheat glucan. Normalized
Page 431 and 432:
Figure 18 Wheat glucan.Elution prof
Page 433 and 434:
Figure 20 Wheat glucans. Absolute m
Page 435 and 436:
Figure 22 Wheat glucan. (a) Intrins
Page 437 and 438:
Figure 24 Wheat PA-glucan. Elution
Page 439 and 440:
an Ubbelohde-viscometer for concent
Page 441 and 442:
Rheological investigations of stabi
Page 443 and 444:
shifts to applied laser light, whic
Page 445 and 446:
Table 8 Characteristic Parameters f
Page 447 and 448:
Table 8 (Continued) and H2O. A part
Page 449 and 450:
10. SG Ball, MHBJ van de Wal, RGF V
Page 451 and 452:
52. W Praznik, R Beck. J Chromatogr
Page 453 and 454:
Page 455 and 456:
3 PROTEIN PARTITIONING IN SEC 3.1 G
Page 457 and 458:
Figure 1 Plot of Kav vs. log M for
Page 459 and 460:
yconsideringtheproteinsolutestobesp
Page 461 and 462:
globular proteins and selected flex
Page 463 and 464:
silanol groups (52-54); even capped
Page 465 and 466:
most proteins to be maximally stabl
Page 467 and 468:
Figure 4 Chromatograms of BSA and P
Page 469 and 470:
concentrated) steps of protein puri
Page 471 and 472:
Table 3 Characteristics of Dextran-
Page 473 and 474:
preparative SEC. The capabilities o
Page 475 and 476:
37. GR Noll, N Nagle, JO Baker, DJ
Page 477 and 478:
Page 479 and 480:
Figure 2 Separation of total E. col
Page 481 and 482:
Figure 3 Separation of HaeIII-cleav
Page 483 and 484:
The recovery of DNA fragments has b
Page 485 and 486:
Endotoxin should also be removed fr
Page 487 and 488:
Table 3 Best Columns for the Separa
Page 489 and 490:
Figure 10 Dependence of HETP on flo
Page 491 and 492:
Appendix (Continued ) Polymer Colum
Page 493 and 494:
REFERENCES 1. CT Wehr, SR Abbott. J
Page 495 and 496:
MALDI-MS has been applied to determ
Page 497 and 498:
Figure 1 Calibration curvesof SEC c
Page 499 and 500:
Figure 3 Chromatograms of epoxy res
Page 501 and 502:
wherenandn0 aretheRIsofthesample an
Page 503 and 504:
Figure 5 SEC chromatogram of n-alka
Page 505 and 506:
Figure 7 Refractive indexes of comm
Page 507 and 508:
Another detector recently applied i
Page 509 and 510:
Figure 11 Absolute MW calibration p
Page 511 and 512:
Figure 12 Chromatogram of Acrawax C
Page 513 and 514:
18 Two-Dimensional Liquid Chromatog
Page 515 and 516:
largerispressuredropandresultingexp
Page 517 and 518:
elations between molar masses and s
Page 519 and 520:
Figure 2 (Continued) chapter, one o
Page 521 and 522:
where Nis the column efficiency exp
Page 523 and 524:
composition/architecture/molar mass
Page 525 and 526:
solvent. Here again, solvent-solven
Page 527 and 528:
or repulsive interactions between m
Page 529 and 530:
macromolecules may strongly differ
Page 531 and 532:
temperature. The efficacy of an ads
Page 533 and 534:
however, aliphatic bonded groups ma
Page 535 and 536:
Figure 10 Schematic representation
Page 537 and 538:
packing materials for SEC of synthe
Page 539 and 540:
Quantitative relations between sila
Page 541 and 542:
Both strength and thermodynamic qua
Page 543 and 544:
where Cand Dare constants for apart
Page 545 and 546:
ThisisimportantwhenLCCCisappliedtoc
Page 547 and 548:
Page 549 and 550:
Page 551 and 552:
However, strong adsorption of macro
Page 553 and 554:
elution step may be sufficient for
Page 555 and 556:
Figure 16 Block scheme of a full ad
Page 557 and 558:
processing of 2D-HPLC data. Knowled
Page 559 and 560:
Figure 20 Set of full retention-elu
Page 561 and 562:
distributions of complex polymers a
Page 563 and 564:
constituents were not discriminated
Page 565 and 566:
(Sec. 7) and therefore they can be
Page 567 and 568:
2. Identify sample solvents. First
Page 569 and 570:
1 Segmental interaction energy para
Page 571 and 572:
58. BG Belenkii. Pure Appl Chem 51:
Page 573 and 574:
19 Methods and Columns for High-Spe
Page 575 and 576:
Table 1 Summary of Methods for Incr
Page 577 and 578:
not savealot of time and solvent, d
Page 579 and 580:
Figure 3 Conventional SEC chromatog
Page 581 and 582:
chromatogram at 4mL/min, which is f
Page 583 and 584:
Sincethereductionoftheinternaldiame
Page 585 and 586:
Table 3 Summary of Column Performan
Page 587 and 588:
Figure 11 Dependence of column perf
Page 589 and 590:
. Two-dimensional chromatography ru
Page 591 and 592:
The standard deviations for the mol
Page 593 and 594:
Figure 15 Comparison of time requir
Page 595 and 596:
Figure 17 Accuracy and precision of
Page 597 and 598:
polymer analysis. Samples are “ru
Page 599 and 600:
just ignoring the fractionation of
Page 601 and 602:
20 Automatic Continuous Mixing Tech
Page 603 and 604:
out at low enough concentration tha
Page 605 and 606:
educed viscosity, h r, to be comput
Page 607 and 608:
© 2004 by Marcel Dekker, Inc. Figu
Page 609 and 610:
Figure 2 Raw ACOMP signals from mul
Page 611 and 612:
Figure 4 Mw vs. monomer conversion,
Page 613 and 614:
decreases smoothly and monotonicall
Page 615 and 616:
Figure 7 Effects of fluctuating rea
Page 617 and 618:
Finally, the use of a light-scatter
Page 619 and 620:
Figure 8 Linear and cubic increases
Page 621 and 622:
where r(t) is now given by 2 6 r(t)
Page 623 and 624:
Figure 11 Light scattering from sol
Page 625 and 626:
3.1 ASimple System: Equilibrium Cha
Page 627 and 628:
Figure 13 (a) ACM applied to the ch
Page 629 and 630:
Also of note in Fig. 13b is how hig
Page 631 and 632:
15. JM Starita, CL Rohn. Rheologica
Page 633 and 634:
52. M Hulden. Hydrophobically modif
Page 635 and 636:
21 Light Scattering and the Solutio
Page 637 and 638:
therefore, is to remove any lingeri
Page 639 and 640:
is often found in the literature wh
Page 641 and 642:
Figure 2 Configuration of an SEC se
Page 643 and 644:
5 THE IMPORTANCE OF SOFTWARE Like a
Page 645 and 646:
standard deviations of the measured
Page 647 and 648:
These include the differential weig
Page 649 and 650:
Figure 8 Data of Fig. 7corrected fo
Page 651 and 652:
Figure 11 Data of Fig. 10 with spik
Page 653 and 654:
Figure 13 Fitstodatacollectedatasli
Page 655 and 656:
Table 1 Errors (in %) Characteristi
Page 657 and 658:
homogeneous copolymer, that is, tak
Page 659 and 660:
process may be used to identify the
Page 661 and 662:
fractionated sample. They represent
Page 663 and 664:
Figure 16 An example of poor SEC ch
Page 665 and 666:
on) methods, whereby the average di
Page 667 and 668:
23. WH Stockmayer, LD Moore Jr, M F
Page 669 and 670:
condition, as is explained in this
Page 671 and 672:
Figure 2 Partition coefficients KL
Page 673 and 674:
4 COLUMNS FOR HOPC Detailsaregiveni
Page 675 and 676:
and concomitant clogging of columns
Page 677 and 678:
Figure 5 Concentration dependence o
Page 679 and 680:
Table 1 Solution Volume, Polymer Ma
Page 681 and 682:
in Fig. 4a. The middle fractions (8
Page 683 and 684:
Figure 10 SEC chromatograms for som
Page 685 and 686:
appropriate for the early fractions
Page 687 and 688:
23 Size Exclusion/ Hydrodynamic Chr
Page 689 and 690:
Figure 2 Calibration curve for the
Page 691 and 692:
Figure 5 Elutionbehaviorofpolystyre
Page 693 and 694:
Table 1 Comparison Between SEC and
Page 695 and 696:
egular SEC column in which the pore
Page 697:
Such an ideal separation can be obt
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