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4. KA Granath, P Flodin. Macromol Chem 48:160, 1961. 5. J Porath. Lab Pract 16:838, 1967. 6. DD Bly. In: B Carroll, ed. Gel Permeation Chromatography in Polymer Chemistry, Physical Methods in Macromolecular Chemistry. Vol. 2. New York, NY: Dekker, 1972. 7. WW Yau, JJ Kirkland, DD Bly. Modern Size Exclusion Chromatography. New York, NY: Wiley, 1979. 8. HG Barth. J Chromatogr Sci 18:409, 1980. 9. JC Giddings. In: JC Giddings, E Grushka, J Cazes, PR Brown, eds. Advances in Chromatography, Vol. 20, New York, NY: Marcel Dekker, 1982, p 217. 10. FE Regnier. Adv Enzymol 91:137, 1983. 11. PL Dubin, JM Principi. Macromolecules 22:1891, 1989. 12. KM Gooding, FE Regnier. HPLC of Biological Macromolecules. New York, NY: Marcel Dekker, 1990. 13. HG Barth, BE Boyes, C Jackson. Anal Chem 70:251R, 1998. 14. RC Montelaro. In: PL Dubin, ed. Aqueous Size Exclusion Chromatography. Amsterdam, The Netherlands: Elsevier, 1988, p 269. 15. KK Unger, JN Kinkel. In: PL Dubin, ed. Aqueous Size Exclusion Chromatography. Amsterdam, The Netherlands: Elsevier, 1988, p 193. 16. K Makino, H Hatano. In: PL Dubin, ed. Aqueous Size Exclusion Chromatography. Amsterdam, The Netherlands: Elsevier, 1988, p 235. 17. KM Gooding, HH Freiser. In: CT Mant, RS Hodges, eds. High Performance Liquid Chromatography of Peptides and Proteins. Boca Raton, FL: CRC Press, 1991, p. 135. 18. GW Welling, S Welling-Wester. In: CT Mant, RS Hodges, eds. High Performance Liquid Chromatography of Peptides and Proteins. Boca Raton, FL: CRC Press, 1991, p. 155. 19. J Porath. Biochem Biophys Acta 39:193, 1960. 20. P Andrews. Nature 196:36, 1962. 21. PG Squire, A Magnus, ME Himmel. J Chromatogr 242:255, 1982. 22. WW Yau, CP Malone, SW Fleming. J Polym Sci 6:803,1968. 23. WW Yau, HL Suchan, CP Malone. J Polym Sci 6:1349, 1968. 24. WW Yau, CP Malone, HL Suchan. Sep Sci 5:259, 1970. 25. JV Dawkins. J Polymer Sci 14:569, 1976. 26. JR Whitaker. Anal Chem 35:1950, 1963. 27. Sephadex Filtration in Theory and Practice. Pharmacia Fine Chemicals, Uppsala, Sweden, 1979. 28. P Andrews. Biochem J 96:595, 1965. 29. AG Ogston. Trans Faraday Soc 54:1754, 1958. 30. PG Squire. Arch Biochem Biophys 107:471, 1964. 31. TC Laurent, J Killander. J Chromatogr 14:317, 1964. 32. JC Giddings, E Kucra, CP Russell, MN Meyers. J Phys Chem 72:4397, 1968. 33. ED Glandt. J Colloid Interface Sci 77:512, 1980. 34. EF Cassassa, Y Tagami. Macromolecules. 2:14, 1969. 35. C Tanford. Physical Chemistry of Macromolecules. New York, NY: Wiley, 1961. 36. ME Himmel, PG Squire. Int J Peptide Protein Res 17:365, 1981. © 2004 by Marcel Dekker, Inc.
37. GR Noll, N Nagle, JO Baker, DJ Mitchell, K Grohmann, ME Himmel. J Liq Chromatogr 13:703, 1990. 38. ME Himmel, PG Squire. In: PL Dubin, ed. Aqueous Size Exclusion Chromatography. Amsterdam, The Netherlands: Elsevier, 1988, p 3. 39. TS Burcham, DT Osuga, H Chino, RE Feeney. Anal Biochem 139:197, 1984. 40. M Dennis, C Lazure, NG Seidah, M Chretien. J Chromatogr 266:163, 1983. 41. C Lazure, M Dennis, J Rochemont, NG Seidah, M Chretien. Anal Biochem 125:406, 1982. 42. JG Bindels, HJ Hoenders. J Chromatogr 261:381, 1983. 43. M Potschka. Anal Biochem 162:47, 1987. 44. PL Dubin, JM Principi. Macromolecules 22:1891, 1989. 45. JL Oncley. In: EJ Cohn, JT Edsall, eds. Proteins, Amino Acids, and Peptides. New York, NY: Reinhold, 1943, Ch. 22. 46. F Perrin. J Phys Radium 7:1, 1936. 47. RO Herzog, R Illig, H Kudar. Z Phys Chem A167:329, 1934. 48. PG Squire, ME Himmel. Arch Biochem Biophys 196:165, 1979. 49. W Kopaciewicz, FW Regnier. In: MTW Hearn, FE Regnier, CT Wehr, eds. High- Performance Liquid Chromatography of Proteins and Peptides: Proceedings of the First International Symposium, Washington, DC, 1981. New York: Academic Press, 1983, p. 151. 50. JE Dyr, J Suttnar. J Chromatogr 408:303, 1987. 51. R Majuri. J Chromatogr 387:281, 1987. 52. W Flapper, AGM Theeuwes, JTG Kierkels, J Steenbergen, HJ Hoenders. J Chromatogr 533:47, 1990. 53. LG Daignault, DC Jackman, DP Rillema. J Chromatogr 462:71, 1989. 54. Y-B Yang, FE Regnier. J Chromatogr 544:233, 1991. 55. B-L Johansson, J Gustavsson. J Chromatogr 457:205, 1988. 56. I Drevin, L Larsson, I Eriksson, and B-L Johansson. J. Chromatogr. 514:137, 1990. 57. KM Gooding, HH Freiser. In: CT Mant, RS Hodges, eds. High-Performance Chromatography of Peptides and Proteins: Separation, Analysis, and Conformation. Boca Raton, FL: CRC Press, 1991, p. 135. 58. M Potschka. J Chromatogr 441:239, 1988. 59. RD Ricker, LA Sandoval. J Chromatogr A 743:43, 1996. 60. PL Dubin. In: PL Dubin, ed. Aqueous Size-Exclusion Chromatography. New York: Elsevier, 1988, p 55. 61. VA Romano, T Ebeyer, PL Dubin. In: M Potshka, PL Dubin, eds. Strategies in Size- Exclusion Chromatography. ACS Symp Ser, 635, 1996, p 88. 62. W Kopaciewicz, MA Rounds, J Fausnaugh, FE Regnier. J Chromatogr 266:3, 1983. 63. JM Park, BB Muhoberac, PL Dubin, J Xia. Macromolecules 25:290, 1992. 64. K Inouye. Agric Biol Chem 55:2129, 1991. 65. L Kagedal, B Engstrom, H Ellegren, A-K Lieber, H Lundstrom, A Skold, M Schenning. J Chromatogr 537:17, 1991. 66. RM Chicz, FE Regnier. Methods Enzymol 182:392, 1990. 67. MA Cohen Stuart, GJ Fleer, J Lyklema, W Norde, JMHM Schleutjens. Adv Colloid Interface Sci 34:477, 1991. © 2004 by Marcel Dekker, Inc.
Page 1 and 2:
MARCEL DEKKER Handbook of Size Excl
Page 3 and 4:
CHROMATOGRAPHIC SCIENCE SERIES A Se
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60. Modern Chromatographic Analysis
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Preface to the Second Edition Gel p
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Preface to the First Edition Molecu
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Contents Preface to the Second Edit
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17. Size Exclusion Chromatography o
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James F. Curry Analytical Departmen
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Iwao Teraoka, Ph.D. Othmer Departme
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The graphical data display typicall
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polymer molecule can elute. The tot
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individual states) allowed to them.
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unsaturation while the IR detector
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Sampleconcentrationshouldbeminimize
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averagesdefinedintermsofthemolecula
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information regarding appropriate M
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Analytical. Two models, the KMX-6 a
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Figure 6 Overlay of time-sliced pea
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accurately for very large and very
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4 GENERAL REFERENCES The interested
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46. TA Chamberlin, HE Tuinstra. US
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materials are commercially availabl
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Table 1 Commercial Column Packing M
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necessarily comparable. For this re
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adjusted such that the pore size di
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narrowmolecular weight range, indiv
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Figure 8 Effect of particle size on
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Figure 9 SEC calibration using poly
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Table 4 Typical Eluent Systems for
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Figure 12 Analysis of poly-2-vinyl
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24. E Meehan, S O’Donohue. The ro
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into accepted laboratory techniques
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Table 1 Trace Metal Impurities in C
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mass and biological activity, for m
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Experimental efficiency vs. velocit
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Table 3 Properties of SEC Silica Ge
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operate at aflow rate that can easi
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Figure 3 Efficiency of amide-bonded
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mechanically stable packing materia
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Figure 5 Pore size distributions of
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Figure 6 Separation of polystyrenes
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60-120 A ˚ are large enough to be
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Table 6 Selected Silica-Based Colum
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Table 7 Separation Ranges for Polym
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the decline in permeability is perm
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sample components can be varied by
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Figure 10 Diol bonding reactions. I
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ecause of the higher relative molec
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valuesfork1,k2,anda,theunknownmolec
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water-soluble and detergent-soluble
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the importance of secondary retenti
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discussed in Table 8(33). Based on
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individual dispersion processes ins
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The detector time constant can dist
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5.3 Mobile Phase A mobile phase is
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less steep and still linear at high
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35. P Bristow, J Knox. Chromatograp
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112. K Gooding, K Lu, G Vanecek, F
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for branched polymers or copolymers
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Figure 1 “Bridge design” flow-r
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Figure 3 A light-scattering photome
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Figure 5 SEC universal calibration
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where hn is the hydrodynamic volume
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It should be noted that dn=dc also
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sensitivity for many samples compar
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that is, log[h] vs. logM,generated
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Figure 8 Effect of band broadening
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Table 1 Measurement of Molecular We
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Figure 10 Differential refractomete
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Figure 11 (A) Mark-Houwink plot of
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Table 3 Measurement of Molecular We
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size information is derived from un
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Table 5 Molecular Weight Distributi
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By measuring the scattered intensit
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8 APPENDIX: INSTRUMENT COMPANIES 8.
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53. G Marot, J Lesec. J Liq Chromat
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125. D Slootmaekers, JAPP Van Dijk,
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200. JG Bindels, GJJ Bessems, BM De
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possibly with hydrodynamic volume (
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An insurmountable limitation of SEC
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to sDR(V) when either nPS ¼nPB ¼c
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1,4-trans;and8%1,2-vynil.TheglobalC
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The goal is to find the MWD and CCD
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As expected, pS is closer to the no
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Figure 3 Example 3: MWD and BD of a
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9. BH Zimm, WH Stockmayer. The dime
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45. RO Bielsa, GR Meira. Linear cop
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solubility properties. They are use
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determination of the absolute molec
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Figure 4 Result of the PBT analysis
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Figure 8 Result of natural spider s
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7 Size Exclusion Chromatography of
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As pointed out earlier, rubber must
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Table 2 Various Solvents and Operat
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Figure 2 Typical GPC calibrations w
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© 2004 by Marcel Dekker, Inc. Figu
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Natural and Synthetic Rubbe177 vari
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Another example, shown in Fig. 5 (3
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APPENDIX: SEC CONDITIONS FOR RUBBER
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Appendix (Continued) Polymer Column
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Page 203 and 204:
Page 205 and 206:
5. J West, E Rodriguez. Rubber Chem
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Page 209 and 210:
associate. Girdler (67) and Speight
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Table 1 Fractions Obtained Using Co
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Figure 1 SEC analyses of an aged as
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Apparently more polar compounds are
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40% was fractionated into four frac
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Figure 7 SEC analyses of an unaged
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Figure 9 Comparison of percentage L
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Figure 11 Comparison of SEC chromat
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Figure 12 Comparisons of SEC chroma
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percentage LMS at the other locatio
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the total area into up to 12 sectio
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attempted to develop an SEC method
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parameterisbasedontheinternalpressu
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5 MODIFIED ASPHALTS The addition of
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Oxidation of the rubber and asphalt
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Figure 20 SEC chromatogram for an a
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Figure 21 Comparisons of apparent m
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© 2004 by Marcel Dekker, Inc. S/
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PS/10 4 þ 10 3 þ 500 þ 100 THF/1
Page 247 and 248:
Nevertheless, SEC of asphalts is es
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56. NW Garrick, RR Biskur. Trans Re
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Asphalts 235 122. M-S Lin, JM Chaff
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Page 255 and 256:
Table 1 Applications of Acrylamide
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(AM/AA), and acrylamide/dimethyldia
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polymer, a large size filter of 5,
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Figure 1 Size exclusion chromatogra
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Table3 MWandMWDofaBroad-MWDPAMStand
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Another commercially available MW d
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Table 5 Weight-Average Molecular We
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Figure 6 (a) Size exclusion chromat
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compared to anormal product. By com
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StudyingtheKineticsofaChemicalReact
Page 275 and 276:
this information, the higher MW pea
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
46. CJ Kim, A Hamielec, A Bendek. J
Page 283 and 284:
Page 285 and 286:
A relatively new technique utilizin
Page 287 and 288:
where ais the exponent of the Mark-
Page 289 and 290:
Figure 2 TDS chromatograms for full
Page 291 and 292:
Figure 5 Comparison of molecular we
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Table 3 Summary of TDS Molecular We
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thepartiallyhydrolyzedPVAwiththecol
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Table 5 Summary of Conformation and
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a¼0.627andlog(K) ¼23.249.Theseare
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The limits of detection of the PVA
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Figure 12 PVAc broad molecular weig
Page 305 and 306:
4 SUMMARY Advances in SEC character
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Page 309 and 310:
2.1 Molecular Weight Grades of PVP
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exclusion chromatography with low-a
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weightandmolecularweightdistributio
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Page 317 and 318:
Figure 4 PEOcalibrationofUltrahydro
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Figure 6 Overlay of GPC chromatogra
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plus Ultrahydrogel 120 A ˚ ,Shodex
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Figure 10 SEC traces of PVP/AA copo
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Figure 11 Molecular weight distribu
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Page 329 and 330:
2 CHEMICAL, MACROMOLECULAR AND MORP
Page 331 and 332:
Table 2 The Relative Composition of
Page 333 and 334:
considered to be 3. After the DS ha
Page 335 and 336:
een extensively studied by methods
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The silylation was performed in LiC
Page 339 and 340:
observed using column materials hav
Page 341 and 342:
Table 6 (Continued) Packing materia
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4.5 Other Cellulose Derivatives Bes
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Table 8 SEC Conditions for Characte
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dextrans swell too much in cadoxen
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stirring at room temperature for an
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Figure 2 MMDofunbleached(HP)andblea
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Figure 4 MMDofbirchkraftpulpdegrade
Page 355 and 356:
adequate when evaluating the influe
Page 357 and 358:
differential refractive index (DRI)
Page 359 and 360:
30. HA Krässig. Effects of structu
Page 361 and 362:
67. E Sjöholm, K Gustafsson, A Col
Page 363 and 364:
104. B Fleury, J Dubois, C Léonard
Page 365 and 366:
136. D Miller, D Senior, R Sutcliff
Page 367 and 368:
166. SS Cutié, CG Smith. Determina
Page 369 and 370:
200. R Berggren, F Berthold, E Sjö
Page 371 and 372:
13 Molar Mass and Size Distribution
Page 373 and 374:
measurements, and vapor pressure os
Page 375 and 376:
The calibration of SEC columns by c
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The division according to eluent ty
Page 379 and 380:
Apolymer produced by UV irradiation
Page 381 and 382:
Because DMAC/LiCl is also agood sol
Page 383 and 384:
compoundsandwasexplainedbyadsorptio
Page 385 and 386:
isolated lignins because they are e
Page 387 and 388:
The Separon HEMA and Separon HEMA B
Page 389 and 390:
Figure 4 GPC elution curves of the
Page 391 and 392:
liquor is highest throughout the co
Page 393 and 394:
topological anisotropy in the polym
Page 395 and 396:
29. M Ristolainen, R Alen, J Knuuti
Page 397 and 398:
eds. Proc Int Symp 1998. Canton, Pe
Page 399 and 400:
92. L Jurasek. Towards a three dime
Page 401 and 402:
Table 1 Selected Industries Connect
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into C3-metabolites, which then may
Page 405 and 406:
Table 4 Key Enzymes in the Biosynth
Page 407 and 408:
Table 5 Cloned Mutants of Starch En
Page 409 and 410:
Figure 3 Modeled x-ray diffraction
Page 411 and 412:
In the native environment (plant ce
Page 413 and 414:
Figure 6 (a) Large starch granules
Page 415 and 416:
number of branching points); crossl
Page 417 and 418:
Both absolute approaches are extrao
Page 419 and 420:
Table 7 (Continued) Approach Experi
Page 421 and 422:
structures, excluded volumes, and t
Page 423 and 424: Figure 10 (a) SEC elution profile o
Page 425 and 426: Figure 12 Wheat glucans: DRI elutio
Page 427 and 428: Figure 13 Wheat glucans. Normalized
Page 429 and 430: Figure 16 Wheat glucan. Normalized
Page 431 and 432: Figure 18 Wheat glucan.Elution prof
Page 433 and 434: Figure 20 Wheat glucans. Absolute m
Page 435 and 436: Figure 22 Wheat glucan. (a) Intrins
Page 437 and 438: Figure 24 Wheat PA-glucan. Elution
Page 439 and 440: an Ubbelohde-viscometer for concent
Page 441 and 442: Rheological investigations of stabi
Page 443 and 444: shifts to applied laser light, whic
Page 445 and 446: Table 8 Characteristic Parameters f
Page 447 and 448: Table 8 (Continued) and H2O. A part
Page 449 and 450: 10. SG Ball, MHBJ van de Wal, RGF V
Page 451 and 452: 52. W Praznik, R Beck. J Chromatogr
Page 453 and 454: 15 Size Exclusion Chromatography of
Page 455 and 456: 3 PROTEIN PARTITIONING IN SEC 3.1 G
Page 457 and 458: Figure 1 Plot of Kav vs. log M for
Page 459 and 460: yconsideringtheproteinsolutestobesp
Page 461 and 462: globular proteins and selected flex
Page 463 and 464: silanol groups (52-54); even capped
Page 465 and 466: most proteins to be maximally stabl
Page 467 and 468: Figure 4 Chromatograms of BSA and P
Page 469 and 470: concentrated) steps of protein puri
Page 471 and 472: Table 3 Characteristics of Dextran-
Page 473: preparative SEC. The capabilities o
Page 477 and 478: 16 Size Exclusion Chromatography of
Page 479 and 480: Figure 2 Separation of total E. col
Page 481 and 482: Figure 3 Separation of HaeIII-cleav
Page 483 and 484: The recovery of DNA fragments has b
Page 485 and 486: Endotoxin should also be removed fr
Page 487 and 488: Table 3 Best Columns for the Separa
Page 489 and 490: Figure 10 Dependence of HETP on flo
Page 491 and 492: Appendix (Continued ) Polymer Colum
Page 493 and 494: REFERENCES 1. CT Wehr, SR Abbott. J
Page 495 and 496: MALDI-MS has been applied to determ
Page 497 and 498: Figure 1 Calibration curvesof SEC c
Page 499 and 500: Figure 3 Chromatograms of epoxy res
Page 501 and 502: wherenandn0 aretheRIsofthesample an
Page 503 and 504: Figure 5 SEC chromatogram of n-alka
Page 505 and 506: Figure 7 Refractive indexes of comm
Page 507 and 508: Another detector recently applied i
Page 509 and 510: Figure 11 Absolute MW calibration p
Page 511 and 512: Figure 12 Chromatogram of Acrawax C
Page 513 and 514: 18 Two-Dimensional Liquid Chromatog
Page 515 and 516: largerispressuredropandresultingexp
Page 517 and 518: elations between molar masses and s
Page 519 and 520: Figure 2 (Continued) chapter, one o
Page 521 and 522: where Nis the column efficiency exp
Page 523 and 524: composition/architecture/molar mass
Page 525 and 526:
solvent. Here again, solvent-solven
Page 527 and 528:
or repulsive interactions between m
Page 529 and 530:
macromolecules may strongly differ
Page 531 and 532:
temperature. The efficacy of an ads
Page 533 and 534:
however, aliphatic bonded groups ma
Page 535 and 536:
Figure 10 Schematic representation
Page 537 and 538:
packing materials for SEC of synthe
Page 539 and 540:
Quantitative relations between sila
Page 541 and 542:
Both strength and thermodynamic qua
Page 543 and 544:
where Cand Dare constants for apart
Page 545 and 546:
ThisisimportantwhenLCCCisappliedtoc
Page 547 and 548:
Page 549 and 550:
Page 551 and 552:
However, strong adsorption of macro
Page 553 and 554:
elution step may be sufficient for
Page 555 and 556:
Figure 16 Block scheme of a full ad
Page 557 and 558:
processing of 2D-HPLC data. Knowled
Page 559 and 560:
Figure 20 Set of full retention-elu
Page 561 and 562:
distributions of complex polymers a
Page 563 and 564:
constituents were not discriminated
Page 565 and 566:
(Sec. 7) and therefore they can be
Page 567 and 568:
2. Identify sample solvents. First
Page 569 and 570:
1 Segmental interaction energy para
Page 571 and 572:
58. BG Belenkii. Pure Appl Chem 51:
Page 573 and 574:
19 Methods and Columns for High-Spe
Page 575 and 576:
Table 1 Summary of Methods for Incr
Page 577 and 578:
not savealot of time and solvent, d
Page 579 and 580:
Figure 3 Conventional SEC chromatog
Page 581 and 582:
chromatogram at 4mL/min, which is f
Page 583 and 584:
Sincethereductionoftheinternaldiame
Page 585 and 586:
Table 3 Summary of Column Performan
Page 587 and 588:
Figure 11 Dependence of column perf
Page 589 and 590:
. Two-dimensional chromatography ru
Page 591 and 592:
The standard deviations for the mol
Page 593 and 594:
Figure 15 Comparison of time requir
Page 595 and 596:
Figure 17 Accuracy and precision of
Page 597 and 598:
polymer analysis. Samples are “ru
Page 599 and 600:
just ignoring the fractionation of
Page 601 and 602:
20 Automatic Continuous Mixing Tech
Page 603 and 604:
out at low enough concentration tha
Page 605 and 606:
educed viscosity, h r, to be comput
Page 607 and 608:
Page 609 and 610:
Figure 2 Raw ACOMP signals from mul
Page 611 and 612:
Figure 4 Mw vs. monomer conversion,
Page 613 and 614:
decreases smoothly and monotonicall
Page 615 and 616:
Figure 7 Effects of fluctuating rea
Page 617 and 618:
Finally, the use of a light-scatter
Page 619 and 620:
Figure 8 Linear and cubic increases
Page 621 and 622:
where r(t) is now given by 2 6 r(t)
Page 623 and 624:
Figure 11 Light scattering from sol
Page 625 and 626:
3.1 ASimple System: Equilibrium Cha
Page 627 and 628:
Figure 13 (a) ACM applied to the ch
Page 629 and 630:
Also of note in Fig. 13b is how hig
Page 631 and 632:
15. JM Starita, CL Rohn. Rheologica
Page 633 and 634:
52. M Hulden. Hydrophobically modif
Page 635 and 636:
21 Light Scattering and the Solutio
Page 637 and 638:
therefore, is to remove any lingeri
Page 639 and 640:
is often found in the literature wh
Page 641 and 642:
Figure 2 Configuration of an SEC se
Page 643 and 644:
5 THE IMPORTANCE OF SOFTWARE Like a
Page 645 and 646:
standard deviations of the measured
Page 647 and 648:
These include the differential weig
Page 649 and 650:
Figure 8 Data of Fig. 7corrected fo
Page 651 and 652:
Figure 11 Data of Fig. 10 with spik
Page 653 and 654:
Figure 13 Fitstodatacollectedatasli
Page 655 and 656:
Table 1 Errors (in %) Characteristi
Page 657 and 658:
homogeneous copolymer, that is, tak
Page 659 and 660:
process may be used to identify the
Page 661 and 662:
fractionated sample. They represent
Page 663 and 664:
Figure 16 An example of poor SEC ch
Page 665 and 666:
on) methods, whereby the average di
Page 667 and 668:
23. WH Stockmayer, LD Moore Jr, M F
Page 669 and 670:
condition, as is explained in this
Page 671 and 672:
Figure 2 Partition coefficients KL
Page 673 and 674:
4 COLUMNS FOR HOPC Detailsaregiveni
Page 675 and 676:
and concomitant clogging of columns
Page 677 and 678:
Figure 5 Concentration dependence o
Page 679 and 680:
Table 1 Solution Volume, Polymer Ma
Page 681 and 682:
in Fig. 4a. The middle fractions (8
Page 683 and 684:
Figure 10 SEC chromatograms for som
Page 685 and 686:
appropriate for the early fractions
Page 687 and 688:
23 Size Exclusion/ Hydrodynamic Chr
Page 689 and 690:
Figure 2 Calibration curve for the
Page 691 and 692:
Figure 5 Elutionbehaviorofpolystyre
Page 693 and 694:
Table 1 Comparison Between SEC and
Page 695 and 696:
egular SEC column in which the pore
Page 697:
Such an ideal separation can be obt
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