Handbook of Size Exclusion Chromatography and Related ...

Table 3 Properties of SEC Silica Gels
Property Common values Range
Particle size, mm 10 5–10
Particle shape
Pore size, A
Spherical Spherical, irregular
˚ 125, 250, 500 60–4000
Specific pore volume, mL/mL a,b
0.40 0.30–0.50
Pore volume, mL/g c
1.2 0.9–1.8
Interparticle porosity, % a
40 35–45
Particle porosity, % a
60 55–80
Surface modification Diol Diol-polyether
a Data from Ref. 33.
b Specific pore volume expressed as mL pore volume per mL column volume.
c Pore volumes (mL/g) of several commercial SEC silica gels.
Pore size (A ˚ )
SW
TSK-GEL
SWXL
TSK-GEL Beckman Bio-Rad
125 1.25 1.00 0.95 0.9
250 1.55 1.30 1.35 1.2
500 1.85 1.50 1.55 1.2
Source: Courtesy of Dr. Paul Shieh (Beckman) and Wai-Kin Lam (Bio-Rad).
silica (50–75%), sodium oxide (1–10%), and boric acid (to 100%), and such
substances as alumina or lime are added to obtain better hydrolytic stability or
larger pore sizes (38).
Silica and its bonded phases are characterized by a variety of techniques,
including chemical, physical, spectroscopic, and chromatographic methods. A
discussion of these techniques can be found in Refs. 39 and 40.
Table 4 Properties of Controlled Porosity Glasses for SEC
Property BIORAN a
CPG b
Pore size, A ˚ 300–4000 75–3000
Specific pore volume, mL/g 0.5–1.2 0.4–0.8
Specific surface area, m 2 /g 10–300 7–340
Particle size, mm 30–250 37–177
Surface modification Diol Diol
a
BIORAN: Schott Glaswerke BioTech, Mainz, Germany.
b
CPG: for address see Ref. 21.
Source: Adapted from Ref. 38.
© 2004 by Marcel Dekker, Inc.