Handbook of Size Exclusion Chromatography and Related ...

Table 2 Characteristics of Agarose and Agarose/Acrylamide Mixed Resins for Protein
SEC
Support name and
manufacturer Vmax a
Selectivity b
pH
Stability
Particle size
(m)
Exclusion
limit (kDt)
Sepharose wc
6B 30 10–4000 4–9 45–165
4B 26 60–20,000 4–9 45–165
2B
Sepharose
15 70–40,000 4–9 60–200
wc CL
6B 30 10–4000 3–14 45–165
4B 26 60–20,000 3–14 45–165
2B 15 70–40,000 3–14 60–200
Superose c 12 Prep Grade 30 1–300 1–14 20–40
Superose c 6 Prep Grade
Ultrogel
30 5–5000 1–14 20–40
d
AcA202 1–15 3–10 60–140 22
AcA54 5–70 3–10 60–140 90
AcA44 10–130 3–10 60–140 200
AcA34
Bio-Gel
20–350 3–10 60–140 750
e
A-0.5m 20 1–500 4–13 40–80
A-1.5m 20 1–1500 4–13 80–150
A-5m 20 10–500 4–13 150–300
A-15m 20 40–15,000 4–13
A-50m 15 100–50,000 4–13
a
Maximal linear velocity (cm/hr) for columns in the 1–6cm diameter range.
b
Selectivity is defined as the fractionation range for globular protein in Daltons.
c
Amersham Biosciences Corp., 800 Centennial Ave., P.O. Box 1327, Piscataway, New Jersey 08855-
1327.
d
Ciphergen Biosystems, Inc., 6611 Dumbarton Circle, Fremont, California 94555.
e
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Herules, California 94547.
6.3 Selection of Hardware
Once the most productive resin has been identified, an appropriately configured
column must be selected. Information about the required throughput, sample
volume, chemical resistance, and cycle time must be included in choice of
column(s). Conventional preparative and process SEC columns (packed or empty)
are available from Amicon/Wright (Danvers, MA), Pharmacia Biotechnology
(Lund, Sweden), TosoHaas (Philadelphia, PA), and Millipore/Waters (Milford,
MA). The Pharmacia Process Stack TM Column PS 370 is the most noteworthy
© 2004 by Marcel Dekker, Inc.