Handbook of Size Exclusion Chromatography and Related ...

Table 3 Characteristics of Dextran-Based Resins for Protein SEC
Support name and
manufacturer V max a
because of the configuration and versatility of the stack (87). The stack may
contain up to six individual columns (37cm 15cm) connected in series. This
translates into a 90-cm bed height or a 96-L total bed column. The separation of
the total bed into a series of discrete 16-L beds allows high throughput and
resolution by supporting the gel and alleviating the bed compression associated
with large bed volumes, while introducing minimal band spreading.
Table 4 Characteristics of Acrylamide and PorousPolystyrene BasedResinsfor ProteinSEC
Support name and
manufacturer Selectivity a
Selectivity b
pH stability
pH stability Particle size (m)
Sephadex c
G-10 ,0.7 2–13 40–120
G-25F 5 1–5 2–13 20–80
G-50F 5 1.5–30 2–13 20–80
G-75 3–80 2–13 20–100
G-100 4–100 2–13 100–300
G-150 5–300 2–13
G-200
PDX
5–600 2–13
d
G.F. 50–150 1–5 50–150
G.F. 50–150 1.5–30 50–150
G.F. 100–300 1.5–30 100–300
G.F. 100–300 1–5 100–300
a
Maximal linear velocity (cm/hr) for columns in the 1–6cm diameter range.
b
Selectivity is defined as the fractionation range for globular protein in kDaltons.
c
Amersham Biosciences Corp., 800 Centennial Ave., P.O. Box 1327, Piscataway, New Jersey 08855-
1327.
d
Polydex Biologicals.
Particle
size (m)
Exclusion
limit (kDt)
Trisacryl b
GF05, M 0.2–2.5 1–11 40–80 3
GF05, LS 0.2–2.5 1–11 80–160 3
GF2000, M 10–15 1–11 40–80 20
GF2000, LS 10–15 1–11 80–160 20
a Selectivity is defined as the fractionation range for globular protein in kDaltons.
b Ciphergen Biosystems, Inc., 6611 Dumbarton Circle, Fremont, California 94555.
© 2004 by Marcel Dekker, Inc.