Handbook of Size Exclusion Chromatography and Related ...

Table 1 Solution Volume, Polymer Mass, and Average Molecular Weights in Each
of Fractions Collected in Separation of PCL10K by a C8-120B Column in Toluene
Fraction Drops
Volume of
solution (mL)
Mass of
polymer (g)
Mw=10 4
(g=mol)
Mn=10 4
(g=mol) Mw=Mn
1 20 0.233 0.0010 2.64 2.38 1.11
2 20 0.223 0.0052 2.17 1.90 1.14
3 20 0.229 0.0153 1.62 1.34 1.21
4 20 0.236 0.0337 1.37 1.08 1.27
5 20 0.243 0.0475
6 20 0.246 0.0532 1.15 0.80 1.43
7 20 0.246 0.0550
8 20 0.245 0.0556 1.05 0.69 1.53
9 20 0.245 0.0558
10 20 0.243 0.0528 0.91 0.57 1.59
11 40 0.476 0.0784
12 40 0.464 0.0458 0.81 0.49 1.63
13 100 1.123 0.0452
14 100 1.127 0.0144 0.60 0.35 1.70
15 300 3.395 0.0091 0.53 0.25 2.08
16 300 3.398 0.0028 0.50 0.17 2.88
Total 1080 12.373 0.5708
increases its concentration gradually to reach a plateau in fraction 9. The plateau
level is nearly equal to the concentration of the injected solution. In toluene, the
increase is more rapid, and the plateau is broader. A decrease in concentration
occurs in the same way for the two solvents except it is delayed in toluene because
of a greater injection volume. Both separations recovered more than 99% of the
Figure 7 HOPC retention curve in separation of PCL10K by a C8-120B column in
dioxane (closed squares) and toluene (open circles).
© 2004 by Marcel Dekker, Inc.