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Chapter III CHN Analysis, FT IR …..
Percentile analysis of C and N for various amounts of different amino-acids
doped KDP crystals is compiled in Table (3.1).
3.3 Paper Chromatography
Table: 3.1 C and N % in different doped sample.
Sample C % N %
Pure KDP _ _
KDP + 0.3 L - histidine 0.17 0.58
KDP + 0.4 L - histidine 0.42 0.87
KDP + 0.5 L - histidine 0.71 0.93
KDP + 0.3 L - threonine 0.13 0.51
KDP + 0.4 L - threonine 0.29 0.78
KDP + 0.5 L - threonine 0.55 0.89
KDP + 0.3DL - methionine 0.20 0.61
KDP + 0.4DL - methionine 0.40 0.85
KDP + 0.5DL - methionine 0.57 1.06
The paper chromatography of dissolved samples of pure and amino acids
doped KDP crystals in water was carried out using nin-hydrin. Paper
chromatograph of doped samples exhibited the characteristic purple color
spot which confirmed the presence of amino acid in the doped sample.
3.4 Infrared Spectroscopy
Infrared spectroscopy is one of the most powerful analytical
techniques, which indicates the possibility of chemical identifications [1]. Till
the early 1980s, instruments for the mid-infrared region were mostly of
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