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Chapter III CHN Analysis, FT IR …..
high-performance liquid chromatography. Most manufacturers offer photo-
acoustic cell along with FTIR as the accessories.
In the present study, the FTIR spectra of pure and L-histidine, L-
threonine and DL-methionine doped KDP crystals were recorded on
BRUKER IFS 66V FT-IR spectrometer, which is shown in Photograph of
figure-(3.6). The spectra of powdered samples in KBr medium were recorded
in the range from 400-4000 cm -1 .
Figure: 3.6 Photograph of BRUKER IFS 66V FT-IR spectrometer.
3. 6 Experimental Study of FTIR Spectroscopy
Infrared spectroscopy is an excellent technique for both qualitative and
quantitative analysis [11-13]. As it has been already noted earlier in this
Chapter, FTIR spectroscopy is the further advancement of IR spectroscopy by
using the mathematical concept of Fourier Transform through proper
electronic circuit and computer interfacing. From the earliest days of infrared
spectroscopy it was observed that functional groups of atoms could be
associated with definite characteristic absorption bands, i.e., the absorption of
infrared radiation over certain frequency intervals. The infrared spectrum of
any given substance is interpreted by the use of the known group frequencies
and thus it will be easy to characterize the substance as one containing a
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