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232 INSTRUMENTAL LABORATORIES AND TECHNOLOGICAL PILOT PLANTS • Transilluminator for electrophoretic gels Biodoc, BIOMETRA (Great Britain) Application: For analysis of electrophoretic gels. • Laminar flow cabinet NU-437-400E, Nu Aire (USA) Application: For work under sterile conditions. • Liquid scintillation counter LS 6000LL, BECKMAN (USA) Application: For determinations of radioactivity in solutions. • Research microscope universal NU, Carl Zeiss Jena (Germany) Application: For examination of cytological preparations. Comments: Universal microscope for transmission and reflected light/polarised light. Magnification from 25x to 2500x. Possibility to apply phase contrast. • Incubator T-303 GF, ASSAB (Sweden) Technical data: 220 V, temperature range – 25-75 o C. Application: For cell cultures under 5% carbon dioxide. • Incubator NU 5500E/Nu Aire (USA) Technical data: 220 V, temperature range from 18 to 55 o C. Application: For cell cultures under 0-20% carbon dioxide. • Laminar flow cabinet V-4, ASSAB (Sweden) Application: For work under sterile conditions. • Image analysis system Komet 3.1, Kinetic Imaging (Great Britain) Application: For comet (single cell gel electrophoresis) analysis. • ISIS 3 Metasystem (Germany) Application: Microscopic image analysis system for chromosomal aberrations (bright field and fluorescence microscopy). VIII. LABORATORY FOR DETECTION OF IRRADIATED FOOD Activity profile: Detection of irradiated food. European standards (CEN) adapted as analytical methods to be routinely used in the Laboratory are based on electron paramagnetic resonance (EPR/ESR) spectroscopy, pulsed photo-stimulated luminescence (PPSL) and thermoluminescence measurements (TL). The research work is focused on the development of these methods as well as on validation and implementation of other detection methods as gas chromatographic determination of volatile hydrocarbons in fats, DNA comet assay (observation of single cells destruction) and statistical germination study. The quality assurance system is adapted in the Laboratory in agreement with the PN-EN 150/IEC 17025:2001 standard and fully documented. Laboratory possesses Accreditation Certificate of Testing Laboratory AB 262 issued by the Polish Centre for Accreditation valid from 25.10.2002 to 25.10.2006. • Thermoluminescence reader TL-DA-15 Automated, Risoe National Laboratory (Denmark) Technical data: turntable for 24 samples, heating range – 50÷500 o C, heating speed – 0.5÷10.0 o C/s, optical stimulated luminescence (OSL) system. Application: Detection of irradiated foods containing silicate minerals, e.g. spices, vegetables shrimps tc, research work on irradiated foods. • Fluorescence microscope OPTIPHOT Model X-2, NIKON (Japan) Technical data: halogen lamp 12 V-100 W LL; mercury lamp 100 W/102 DH; lenses (objectives) CF E Plan Achromat 4x, CF E Plan Achromat 40x, CF FLUOR 20x. Application: Detection of irradiated foods by the DNA comet assay method, research work on apoptosis in mammalian cells, biological dosimetry, analysis of DNA damage in mammalian cells. • Compact EPR spectrometer EPR 10-MINI, St. Petersburg Instruments Ltd. (Russia)
INSTRUMENTAL LABORATORIES AND TECHNOLOGICAL PILOT PLANTS 233 Technical data: sensitivity – 3x10 10 , operating frequency (X band) – 9.0-9.6 GHz, max. microwave power – 80 mW, magnetic field range – 30-500 mT, frequency modulation – 100 kHz. Application: Detection of irradiated foods, bone and alanine dosimetry, research work on irradiated foods and bone tissues. • Pulsed photo-stimulated luminescence system SURRC (United Kingdom) Technical data: pulsed light source – diodes IR LED; detector – photomultiplier ETL; pulse on and off periods – 15 µs; sample holder – 50 mm diameter disposable Petri dishes; set up – sample chamber and detector head assembly, contol unit, on line computer, optional. Application: Irradiated food screening system. IX. EXPERIMENTAL PLANT FOR FOOD IRRADIATION 1. Microbiological Laboratory Activity profile: optimization of food irradiation process by microbiological analysis. • Sterilizer ASUE, SMS (Warszawa, Poland) Application: Autoclaving of laboratory glass, equipment, and microbiological cultures. • Fluorescence microscope BX, Olimpus (Germany) Application: Quantitative and qualitative microbiological analysis. 2. Experimental Plant for Food Irradiation Activity profile: Development of new radiation technologies for the preservation and hygienization of food products. Development and standarization of the control system for electron beam processing of food. Development of analytical methods for the detection of irradiated food. Organization of consumer tests with radiation treated food products. • Accelerator ELEKTRONIKA (10 MeV, 10 kW) UELW-10-10, NPO TORIJ (Moscow, Russia) Application: Food irradiation.
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ANNUAL REPORT 2005 50 years in the
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CONTENTS GENERAL INFORMATION 9 MANA
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DETERMINATION OF CADMIUM, LEAD, COP
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NUCLEONIC CONTROL SYSTEMS AND ACCEL
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GENERAL INFORMATION 9 GENERAL INFOR
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MANAGEMENT OF THE INSTITUTE 11 MANA
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MANAGEMENT OF THE INSTITUTE 13 •
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SCIENTIFIC STAFF 15 5. Danilczuk Ma
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RADIATION CHEMISTRY AND PHYSICS, RA
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20 RADIATION CHEMISTRY AND PHYSICS,
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RADIOCHEMISTRY, STABLE ISOTOPES, NU
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RADIOBIOLOGY
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100 RADIOBIOLOGY THE ROLE OF LYSOSO
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102 many found the ratio to be high
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104 RADIOBIOLOGY DNA INTER-STRAND C
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106 SIRTUIN INHIBITION INCREASES TH
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108 The possible reason of this eff
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NUCLEAR TECHNOLOGIES AND METHODS 11
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THE INCT PUBLICATIONS IN 2005 143 T
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THE INCT PUBLICATIONS IN 2005 145 2
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THE INCT PUBLICATIONS IN 2005 CHAPT
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THE INCT PUBLICATIONS IN 2005 163 V
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NUKLEONIKA 169 NUKLEONIKA THE INTER
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NUKLEONIKA 171 7. Influence of time
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INTERVIEWS IN 2005 173 INTERVIEWS I
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CONFERENCES ORGANIZED AND CO-ORGANI
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Page 177 and 178: CONFERENCES ORGANIZED AND CO-ORGANI
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Page 213 and 214: LECTURES AND SEMINARS DELIVERED OUT
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Page 231: INDEX OF THE AUTHORS 235 Lisowska H
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