1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
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Session <strong>1.</strong> Biotechnology and synthetic biology<br />
A B S T R A C T B O O K – A B S T R A C T S O F T A L K S<br />
PHYTOREMEDIATION OF NITROESTERS<br />
Radka Podlipná 1 , Eliška Nachlingerová 2 , Tomáš Vaněk 1<br />
1 Laboratory of Plant Biotechnologies, Experimental Botany CAS, Prague, Czech Republic<br />
2 Faculty of Environmental Technology, Institute of Chemical Technology, Prague, Czech Republic<br />
E-mail: podlipna@ueb.cas.cz<br />
Explosives are toxic, recalcitrant to degradation and contaminate large areas of land and<br />
ground water. Remediation of these xenobiotics is difficult and an enormous logistical<br />
task. Phytoremediation is a technique that offers an environment friendly, low-cost<br />
alternative to current remediation techniques. However this approach is hindered by the<br />
low metabolic abilities of plants towards these xenobiotic compounds and the<br />
phytotoxicity of these compounds. Pentaerythritol tetranitrate (PETN) is one of the most<br />
powerful detonated explosives. The ecotoxicity of PETN was characterized by the test<br />
based on the inhibition of elongation of primary roots 3days-old mustard and hemp<br />
seedlings. The fate of PETN on exposure to plant was investigated in hydroponic and in<br />
vitro systems. Medium and extracts of plants were analyzed by HPLC (High-performance<br />
liquid chromatography). This study showed that PETN was taken up by plant species in<br />
both systems. The accumulation of this nitroester was observed more in the roots than in<br />
the aerial parts of the plants.<br />
Acknowledgments<br />
The authors thank for financial support of projects 1MPO6030 and grant MYES of CR n. OC1002.<br />
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