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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S ICDH seems to be greatly modulated by reactive nitrogen species (RNS) in both organs, whereas this isoenzyme is only sensitive to redox and oxidative changes in Arabidopsis leaves. DYNAMICS OF PLANT HORMONES DURING DEVELOPMENT OF WHEAT CARYOPSES P. Dobrev 1 , J. Albrechtová 2 , M. Trčková, V. Motyka 1 , M. Kamínek 1 1 Institute of Experimental Botany, AS CR, Prague, Czech Republic 2 Department of Plant Physiology, Faculty of Science, Charles University, Prague, Czech Republic 3 Crop Research Institute, Prague, Czech Republic E-mail: kaminek@ueb.cas.cz Discovery of cytokinin (CK) accumulation in young seeds shortly after pollination has attracted attention to the potential role of plant hormones in control of seed development. In addition to CKs, transient increases in the contents of indole-3-acetic acid (IAA) and its conjugates as well as of abscisic acid (ABA) were found during the latter anatomically distinguished phases of seed development. Using advanced HPLC/MS we determined the dynamics of concentrations of CKs, IAA, ABA and some of their metabolites in developing wheat grains and related them to the changes in grain anatomy (coenocyte mitosis, endosperm cellularization, development of starchy endosperm and grain filling). Results indicate that CK/auxin ratio, that is evidently important for initiation of specific anatomical events, is enhanced/adjusted not only by biosynthesis or degradation of a particular hormone (as CKs shortly after anthesis) but also by inactivation of the hormonal counterpart (auxin) due to its conjugation. Results will be discussed with respect to our recent detection of CK biosynthesis in wheat grains shortly after anthesis using stable isotope non-specific labeling following in vivo feeding with 2H2O. CLONING AND CHARACTERIZATION OF TWO SERK GENES TO INVESTIGATE THE MOLECULAR BASIS OF ORGANOGENESIS AND SOMATIC EMBRYOGENESIS IN CYCLAMEN PERSICUM M. Savona 1,3 , R. Mattioli 2 , S. Nigro 2 , G. Falasca 1 , F. Della Rovere 1 , P. Costantino 2 , S. De Vries 4 , B. Ruffoni 3 , M. Trovato 2 , M.M. Altamura 1 1 “Sapienza” University of Rome, Dept. of Biology and Biotechnology, Rome, Italy 2 “Sapienza” University of Rome, Dept. of Environmental Biology, Rome, Italy 3 C.R.A.-Research Unit for Floriculture and Ornamental Species, Sanremo, Italy 4 Wageningen University, Laboratory of Biochemistry, Wageningen, the Netherlands E-mail: maurizio.trovato@uniroma<strong>1.</strong>it Somatic embryogenesis and organogenesis in vitro are essential for propagation, particularly for those species, such as Cyclamen spp., which can be only propagated in vitro. However, the genetic basis of the specification of the respective stem cells remains obscure. Since SOMATIC EMBRYOGENESIS RECEPTOR- LIKE KINASE (SERK) genes are involved in somatic embryogenesis as well as, in some species, in organogenesis, we searched for AtSERK homologs, in in vitro culture of Cyclamen persicum immature ovules. By a combination of 5'- and 3' Race approaches on RT-PCR templates, and of genome walking and IP-PCR strategies on genomic DNA templates, we eventually identified full cDNA and genomic sequences of two SERK-like genes named CpSERK1 (GenBank accession no. JF511659), and CpSERK2 (GenBank accession no.GU189408), as well as a partial cDNA fragment similar to AtSERK3 (GenBank accession no. EF661828). As in vitro culture of Cyclamen persicum immature ovules provide, in the same genotype and hormonal conditions, either lines forming organs and/or embryos or recalcitrant calli, this study aims to use CpSERK1 and CpSERK2 as tools to understand the molecular basis of stem cell formation and maintenance in somatic embryogenesis and organogenesis in vitro. PLANT PROTEINS CONTAINING LEUCINE-RICH REPEAT (LRR) WITH NON-LRR, ISLANDS INTERRUPTING LRRS Norio Matsushima, Tomoko Mikami, Hiroki Miyashita 65 X X I V S P P S C O N G R E S S 2 0 1 1
Sapporo Medical University, Sapporo, Japan E-mail: matusima@sapmed.ac.jp A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S LRRs (leucine rich repeats) are present in over 50,000 proteins. Non-LRR, island regions (IRs) interrupting LRRs are widely distributed. Here we describe LRR@IR proteins from various plant species, identified by a method to identify IRs (LRR@IRpred). A great number of LRR-RLKs and LRR-RLPs, that contain a single transmembrane-spanning region, have LRRs intersected by a single IR in which the number of repeat units in the preceding LRR block (N1) is greater than the number of the following block (N2); N1 " N2. Examples include; the A. thaliana TMK1 homologs have 13 LRRs with N1 = 10 and N2 = 3; and A. thaliana BRI1-related proteins have 22 LRRs with N1 = 17 and N2 = 5. The homologs of A. thaliana BRI1, A. thaliana RPP27, and tomato Cf have very variable N1, while N2 is highly conservative; N1 = 14 - 33 and N2 = 4. The IRs in the homologs of TMK1, BRI1 and BRI1-related proteins contains cysteine clusters that likely form a cap structure. The rule of N1 " N2 would play a novel, significant role in ligand-interaction and/or dimerization of the LRR- RLKs and the LRR-RLPs. The structure and evolution of plant LRR@IR proteins also are discussed. PROTEOMICS OF PEPPER (CAPSICUM ANNUUN L.) FRUITS DURING RIPENING Paz Alvarez 1 , Ana Jiménez 2 , Mounira Chaki 1 , Daniel Bonilla-Valverde 1 , María J. Campos 1 , Luis A. del Río 1 , Francisca Sevilla 2 , Francisco J. Corpas 1 , José M. Palma 1 1 Estacion Experimental del Zaidin, CSIC, Granada, Spain 2 Centro de Edafología y Biología Aplicada del Segura, CSIC, Murcia, Spain E-mail: paz.alvarez@eez.csic.es Pepper (Capsicum annuum L.) is one of the most consumable worldwide vegetable and its fruits are rich in vitamins C, A and calcium. In pepper the main ripening visible feature is the shift from green to red fruits due to the conversion of chloroplasts into chromoplasts. Besides, during fruit ripening other important metabolic changes have been reported such as taste alteration, emission of volatile organic compounds, destruction of chlorophyll, synthesis of new pigments and pectins, synthesis of new proteins and degradation of former ones, changes in total soluble reducing equivalents, and alteration of organelles' metabolism (chloroplasts, peroxisomes and mitochondria), among others. In spite of that all those processes have been reported, the information of the events which take place at molecular level is still scarce. Proteomics has been recently proposed as a very useful tool to the understanding of fruit ripening and development [1]. Thus, the analysis of the proteome of both green and red pepper fruits has been accomplished in this work. Whole fruits have been studied, but also the sub-proteome of both peroxisomes and mitochondria from both kinds of fruits have been investigated. Acknowledgements Financed by the Ministry of Science and Innovation (AGL2002-00834), Spain References [1] Palma et al., J. Proteomics2011 STIMULATION OF ROOT GROWTH INDUCED BY ALUMINUM IN QUERCUS SERRATA THUNB. IS RELATED TO ACTIVITY OF NITRATE REDUCTASE AND MAINTENANCE OF IAA CONCENTRATION IN ROOTS Rie Tomioka, Mikiko Kojima, Hitoshi Sakakibara, Chisato Takenaka, Masayoshi Maeshima, Takafumi Tezuka Nagoya University, Nagoya, Japan E-mail: tomiokar@agr.nagoya-u.ac.jp Aluminum is the most abundant metal in the earth's crust. Excess Al 3+ released by soil acidification in soil solution is toxic to many cultivated plant species, but it has been reported to stimulate plant growth in some crop and tree species in certain concentration of Al 3+ . In this study, we investigated the mechanism of Al-induced root development, focusing on the change in NR activity, and indole-3-acetic acid (IAA) and cytokinins concentration in roots of Q. serrata seedlings. NR activity in Al-treated roots was increased 3 days after treatment. To clarify the process of Al-induced root development, roots were treated for 1 h with Al or 66 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Page 17 and 18: Session 1. Biotechnology and synthe
Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 53 and 54: Session 13.Pathogen defense and pla
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Page 93 and 94: A B S T R A C T B O O K - L I S T O
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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