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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Here we summarize our recent results concerning a role of NPC in responses of plants to abiotic stresses mainly to aluminium and salt. Aluminium inhibited NPC activity in both tobacco BY-2 cells and pollen tubes. The inhibition was time and concentration dependent. Aluminium arrested growth of pollen tubes was rescued by diacylglycerol (DAG) treatment. Treatment with phosphatidic acid was ineffective. Salt stress caused rapid, concentration dependent and transient upregulation of NPC4 expression in Arabidopsis roots. NPC4prom::GUS experiments revealed root tip localization of NPC4 expression. The in situ activity of NPC increased after salt treatment in Arabidopsis seedlings in time and concentration dependent manner as well. The expression of ABA related genes in salt treated NPC4 knockout plant was impaired. The possible role of DAG will be discussed. IMPORTANCE OF PHOSPHORYLATION SITES IN SAMDC UORF PEPTIDE FOR MAINTAINING THE HOMEOSTATIC POLYAMINE LEVELS BY THE REGULATION OF DEGRADATION OF ITS OWN MRNA TRANSCRIPT AND PROTEIN Yu Jin Choi, Ky Young Park Sunchon National University, Jeollanam-do, South-Korea E-mail: plpm@sunchon.ac.kr S-Adenosylmethionine decarboxylase (SAMDC), a key enzyme for polyamines biosynthesis, was tightly regulated for homeostatic levels by translational inhibition of its own protein. The degradation of downstream GUS mRNA in transgenic tobacco plants containing point-mutation of phosphorylated small uORF peptide at Ser10 (P10), Ser17 (P17), Ser28 (P28) and Ser54 (P54), respectively, were about <strong>1.</strong>8-fold delayed than WT uORF plants. These data suggest that phosphorylation of small uORF peptide is an essential component for maintaining the transcript level of the downstream ORF. Also, we showed that degradation rate of GUS and SAMDC protein after treatment with protein synthesis inhibitor cycloheximide and specific proteasome inhibitor MG115 was accelerated in the presence of small uORF peptide. These results implied that small uORF peptide might effectively act as a functional regulator for its related ORF in cis in the manner of proteasome-dependent degradation. However, these effects of SAMDC uORF protein did not occurred on endogenous activity of ADC, which was other enzyme for polyamine biosynthesis and contained its own ADC uORF sequence in 5' UTR. Therefore, SAMDC uORF has a sequence-specific function of transcriptional and/or translational inhibitor for its own SAMDC protein not only in cis but also in trans. CHARACTERIZATION OF CYSTEINE-RICH RLKS IN ARABIDOPSIS THALIANA Niina Idänheimo 1 , Adrien Gauthier, Mikael Brosché 1,2 , Hannes Kollist 2 , Jaakko Kangasjärvi 1 , Michael Wrzaczek 1 1 University of Helsinki, Helsinki, Finland 2 University of Tartu, Tartu, Estonia E-mail: niina.idanheimo@helsinki.fi The production of reactive oxygen species (ROS) is a common response to many stresses in plants. The production and the action of ROS are critical to the stress recognition and the coordination of the plant's response to those stresses. Plants can suffer from oxidative stress when ROS production exceeds antioxidizing capacity and can ultimately lead to cell death by ROS toxicity. In contrast to ROS as toxic molecules, ROS is also a signalling molecule and important regulator of plant development and controls processes such as root development, pollen tube growth and cell expansion. ROS are sensed via yet unidentified mechanisms. We have found several Cysteine-rich RLKs (CRKs), which are transcriptionally regulated in response to ROS. The members of the CRK subfamily could act as ROS “receptors” by sensing ROS through redox modifications of the extracellular domain which could activate receptor complex and lead to signal transduction and response. Phenotypic analysis of the crk knock-out and the overexpression plants under various conditions have provided more information about the biological relevance of these proteins and supported our hypothesis. We have started physical and molecular characterization of these proteins to analyze their role in ROS perception and subsequent signal transduction. 71 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S THE ROLE OF F-BOX-PROTEINS IN STRESS SIGNALING IN ARABIDOPSIS THALIANA Maria Piisilä, Mehmet Ali Keçeli, Günter Brader, Tapio Palva Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland E-mail: maria.piisila@helsinki.fi Induced defenses and stress responses play a major role in plant disease resistance and are regulated by a network of interconnected signal transduction pathways with the plant hormones ethylene (ET), jasmonic acid (JA) and salicylic acid (SA) as crucial mediators. These specific hormone-mediated signaling cascades trigger distinct sets of stress-related genes leading to tolerance or resistance. F-box proteins (roughly 700 in Arabidopsis) are important components in these stress responses. They act as members of SCF protein complexes, which target substrate proteins for modification and degradation and thereby allow plants to respond rapidly to environmental stress factors. They are involved in various processes including plant metabolism, hormonal responses as well as responses to environmental stresses. The objective of this study is to identify and characterize specific F-Box proteins involved in stress signaling and components interacting with these F-box proteins. To elucidate the function of F-box proteins belonging to subfamily C3 in the regulation of stress responses, we employ a reverse genetic screening approach and characterize T- DNA mutant lines of this subfamily of F-box proteins for their performance under abiotic (cold, ozone, drought) and biotic (Erwinia carotovora, Botrytis cinerea, Alternaria brassicicola) stresses. Mutants with altered stress response phenotypes will be subjected to microarray analysis to elucidate the response pathways a given F-box protein is involved in. So far, more than 50 F-Box mutant lines were screened with the pathogens Erwinia carotovora and Botrytis cinerea and cold, ozone, salt and different hormones. We have found many candidates which are showing clear phenotype to pathogen (Erwinia), ozone, and hormone (ABA) treatments. Our research is focusing on these confirmed candidates. Acknowledgements This work is supported by the Academy of Finland Center of Excellence program. EMBRYO-SPECIFIC PROTEINS IN CYCLAMEN PERSICUM ANALYZED WITH 2-D DIGE Ragnhild Lyngved, Jenny Renaut, Jean-Francois Hausman, Tor-Henning Iversen, Anne Kathrine Hvoslef-Eide Sør-Trøndelag University College, Trondheim, Norway E-mail: ragnhild.lyngved@hist.no Somatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one of the most important ornamental plants for the European market, both as a potted plant in northern Europe and a bedding plant in the cool winters in southern Europe. The aim of this study was to obtain new insights into the molecular biology of somatic embryogenesis, which in turn can be useful for the improvement of tissue culture methodology. Total proteins were characterized from two isogenic cell lines of Cyclamen persicum, one that was embryogenic and one that never has shown any embryogenic capacity. The extracted proteins were separated by two-dimensional differential gel electrophoresis (2-D DIGE) and selected proteins were treated using the ETTAN Dalt Spot Handling Workstation. Protein identification was performed using MALDI-TOF-MS. More than 1200 Cyclamen proteins were detected; 943 proteins were common to both lines. The different protein patterns of the embryogenic and non-embryogenic cell lines were obvious: One hundred eight proteins were more abundant in the embryogenic cells, and 97 proteins in the non-embryogenic cells. Among the differentially expressed proteins, 128 were identified. MALDI-TOF- MS analysis enabled 27 spots to be proposed as candidates for embryo-specific proteins, as they were unique to the embryogenic cell line. The proteins identified are involved in a variety of cellular processes, including cell proliferation, protein processing, signal transduction, stress response, metabolism, and energy state, but the majority are involved in protein processing and metabolism. The main functions of the putative embryo-specific proteins have been discussed in proportion to their role in the somatic embryogenesis process. STUDYING THE MECHANISMS UNDERLYING SUCROSE-DEPENDENT GENE EXPRESSION IN A. THALIANA CELL CULTURES 72 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
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Page 37 and 38: Session 7.Development A B S T R A C
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Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 91 and 92: SCF = A B S T R A C T B O O K - A B
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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