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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S UGT85K5 were able to glucosylate acetone cyanohydrin and 2-hydroxy-2methylbutyronitrile forming linamarin and lotaustralin, and furthermore displays broad in vitro substrate specificity as shown by their ability to glucosylate other hydroxynitriles, some flavonoids and simple alcohols. Immunolocalization studies of UGT85K4 and UGT85K5 indicated co-occurrence with the enzymes catalyzing the first two steps in the cyanogenic glucoside biosynthesis, CYP71E7 paralogs and CYP79D1/D2, in mesophyll and xylem parenchyma cells in the first unfolded leaf of cassava. Furthermore In situ PCR showed that UGT85K4 and UGT85K5 are co-expressed with CYP79D1 and both CYP71E7 paralogs in the cortex, phloem and xylem parenchyma and in specific cells in the endodermis of the petiole of the first unfolded leaf. PINOSYLVIN BIOSYNTHESIS IN SCOTS PINE (PINUS SYLVESTRIS) Tanja Paasela, Kean-Jin Lim, Teemu H. Teeri Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland E-mail: tanja.paasela@helsinki.fi The stilbene pinosylvin is a phenolic compound that is synthesised in Scots pine under both developmental and stress induced control. In heartwood of adult trees pinosylvin concentration correlates with decay resistance; trees that have the highest amount of stilbenes in their heartwood are the most resistant ones [1]. Our aim is to find out what regulates pinosylvin formation in adult trees when heartwood is developing and in young seedlings in stress conditions. Do these two processes have common genetic determinants, i.e. are they regulated by converging signal transduction pathways? Finding genetic causes for the variation in heartwood stilbenes would make early selection of seedlings with DNA markers possible. UV-C light activates transcription of pinosylvin synthase gene (PST-1) within hours after treatment in sixweek-old seedlings. Protein translation inhibitor cycloheximide doesn't have an effect on transcriptional activation of PST-1 under UV stress which infers that specific transcription factors are already present in uninduced tissues. Wounding activates PST-1 transcription in xylem of five-year-old seedlings. Transcription is perceivable 24 hours after wounding. Additionally, osmotic stress induces transcription of PST-1 in seedlings. References [1] Venäläinen et al., Ann.Forest Sci. 2003 METABOLOMICS REVEALS A SIGNIFICANT METABOLIC SHIFT IN ARABIDOPSIS PLANTS GROWN IN THE FIELD AND CLIMATE CHAMBERS Yogesh Mishra 1,2 , Hanna Johansson Jänkänpää 1 , Christiane Funk 2 , Wolfgang Peter Schröder 1,2 , Stefan Jansson 1 1 Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden 2 Umeå Plant Science Centre, Department of Chemistry, Umeå University, Umeå, Sweden Email: stefan.jansson@plantphys.umu.se, yogesh.mishra@plantphys.umu.se Plants are most consummate and sophisticated system in world, with tremendous capacity to adjust them self against the natural variation, called as phenotypic plasticity. Therefore, it is utmost import to understand their adaptation capacity in natural condition (where they actually developed) rather in growth chamber. In previous study (Mishra et al, unpublished) we find that Arabidopsis growing in field are significantly differ from growth chamber in terms of leaf morphology and photo system components. The metabolome provides a tool for understanding the function of gene even if that gene has minimal or no effect on phenotype. Therefore, to achieve exclusive insights in above differences into the metabolic level, we compare the metabolite profiles of leaves of wild type Arabidopsis thaliana (Col) growing under constant laboratory conditions and field. Sugar and sugar derivatives fructose, sucrose, glucose, galactose and rafinose registered ten fold accumulations in climate chamber over filed grown plants. However, the levels of aminoacids display large increase in field grown plants. Several intermediates of TCA cycle including succinate, fumarate and malate three fold higher in growth chamber compared to field grown plants. The above changes in metabolites suggest a metabolic shift has occurred. This finding provides new insight into the mechanism of plant adaptation at metabolomic level, and highlights the role of known protectants under natural conditions. 85 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S STEM HYDRAULIC CONDUCTANCE IS INFLUENCED BY LIGHT - EXPERIMENTAL EVIDENCE FROM SILVER BIRCH (BETULA PENDULA) Eele Õunapuu, Arne Sellin Department of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia E-mail: ounapuu@ut.ee Stem hydraulic conductance (Kstem) has previously been shown to depend mainly on the anatomical features of xylem conduits and the possibility of rapid flow control in xylem has not been considered. Here we report evidence for short-term changes in Kstem for silver birch (Betula pendula) related to light conditions. First we sampled shoots cut from lower (shade shoots) and upper (sun shoots) thirds of naturally growing ~25-year-old forest trees. Before hydraulic measurements the shoots were exposed to photosynthetic photon flux density (PPFD) of 70, 140, 330 or 610 µmol m -2 s -1 for 7 h. Both canopy position (long-term effect) and incident PPFD (short-term effect) had a significant impact on Kstem. Sun shoots exhibited consistently higher Kstem compared with shade shoots. For both canopy positions maximum values of Kstem were recorded at PPFD of 330 µmol m -2 s -1 . In a second experiment conducted in 4-year-old saplings growing in an experimental plantation, Kstem as well as potassium ion concentration ([K + ]) in xylem sap varied considerably along the canopy vertical profile, both increasing from bottom to top in accord with the light availability gradient in the canopy; there existed a strong relationship between Kstem and [K + ]. These results suggest that Kstem is dynamic on a short time scale and that potassium is involved in the regulation of Kstem in relation to light availability. FRUCTAN REGULATION IN TIMOTHY (PHLEUM PRATENSE L.) AS AFFECTED BY STAGES OF DEVELOPMENT AND BY WILTING M. Ould Baba Ahmed 1,2 , A. Morvan-Bertrand 1 , Ml. Decau 1 , C. Lafreniere 2 , Mp Prud'homme 1 , P. Drouin 2 1 Ecophysiologie Végétale, Agronomie et nutritions NCS, Esplanade de la Paix, Université de Caen, Caen cedex, France 2 Unité de Recherche et de Développement en Agroalimentaire en Abitibi-Témiscamingue, UQAT, Rouyn-Noranda, Québec, Canada E-mail: Marouf.Ould-Ahmed@uqat.ca In temperate grasses chloroplastic starch accounts for about 15% of non-structural carbohydrate (NSC). The predominant NSCs are vacuolar sucrose and fructans. Fructans are known to have a role in physiological responses to low temperatures and regrowth after defoliation. Fructan degradation during silage contributes to forage preservation and studies suggest that plant fructan exohydrolases (FEHs) are partially responsible for that degradation. However, fructan breakdown by plant enzymes in harvested tissues as well as the availability of soluble sugars during storage are little documented. Here, FEH activity and NSC profiles, including starch, were evaluated during development (vegetative growth, stem elongation, heading and anthesis) and during 24 hours of wilting after cutting for the last two stages in leaves and stems of timothy. This study shows that whereas starch content decreases, fructan content increases with growth being maximal at anthesis in N non-limiting conditions. After cutting, fructans content was relatively stable and FEH activity low, possibly due to desiccation. Besides, three putative FEH coding genes were isolated from cDNA library on these same tissues. Expression analysis of the putative FEH transcript levels and functional characterization by heterologous expression in Pichia pastoris of the corresponding genes are in progress to complete this study. MICROTUBULE (+)-END-ASSOCIATED PROTEIN FROM SOLANACEAE INTERACTING WITH POTYVIRAL HELPER COMPONENT PROTEINASE Tuuli H. Haikonen, Minna-LiisaRajamäki, Jari P. T. Valkonen Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland E-mail: Tuuli.Haikonen@helsinki.fi 86 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
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Pleanary session 4 A B S T R A C T
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Invited talk 4 A B S T R A C T B O
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Session 5.Cell biology A B S T R A
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Session 6.Organelle biology A B S T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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