1. Front Cover.cdr - CORE

Recommendations

Info

A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S showed the accumulation of new compounds due to the Δ5,7-sterolΔ7-reductase activity on ergosterol and its Δ5,7-sterol precursor. TEMPERATURE DEPENDENCE OF RUTIN-FE(II) BINDING CONSTANTS DETERMINED BY ISOTHERMAL TITRATION CALORIMETRY Ingunn W. Jolma, Cathrine Lillo, Peter Ruoff Center for Organelle Research, University of Stavanger, Stavanger, Norway E-mail: ingunn.w.jolma@uis.no Flavonoids are an important class of plant secondary metabolites, which not only set colors on fruits and vegetables, but also play important roles as antioxidants and are considered to promote human health. Flavonoid glycosides, such as rutin (quercetin-3-rutinoside) can also bind cations, such as Fe(II), effectively reducing the Fenton reaction which would otherwise form reactive oxygen species (ROS). This ability to bind iron ions might also play an important role in modulating iron homeostasis. The binding between quercetins and a variety of cations, especially Fe(II) was the subject of several studies indicating that the stability constant was quite large. Using isothermal titration calorimetry (ITC), we investigated the stability constant between rutin and Fe(II) at pH 7.0 at various temperatures in the range between 20-40 degrees Celsius. The results indicate that Fe(II) strongly binds rutin, and that the binding constant decreases with increasing temperature. Stoichiometries indicate that Fe(II) has the possibility to bind up to two molecules of rutin, which is favoured at low temperatures, while at higher temperatures the equilibrium is shifted in direction to a 1:1 Fe(II)-rutin complex. The ITC experiments showed that the formation of the Fe(II)-rutin complexes are associated with large exothermic enthalpies. IMPACT OF GLUCOSE 1-PHOSPHATE POOLS ON STARCH METABOLISM IN HIGHER PLANTS Joerg Fettke University of Potsdam, Potsdam, Germany E-mail: fettke@uni-potsdam.de For several glucosyl transfer reactions glucose 1-phosphate is an essential metabolite that acts either immediately as glucosyl donor or as a substrate for the formation of the more general donors, ADPglucose and UDPglucose. New data revealed that a glucose 1-phosphate transport over both the plasma- and plastidial membrane is possible [1,2]. The glucose 1-phosphate uptake by the cells and the import into intact plastids is highly specific for the anomeric position of the phosphate ester as glucose 6-phosphate does not substitute the carbon 1 ester. Glucose 1-phosphate is taken up by both autotrophic and heterotrophic cells (such as mesophyll protoplasts and potato tuber parenchyma cells). Following uptake, glucose 1-phosphate is in part metabolized in the cytosol [3] but the majority of the glucose 1-phosphate is imported into the plastids and subsequently enters the plastidial path(s) of starch biosynthesis. The import into plastids as well as the conversion of glucose 1-phosphate to starch has been characterized by both in situ and in vitro experiments. References [1]Fettke et al. New Phytol. 2010 [2]Fettke et al. Plant Physiol. 2011 [3]Fettke et al. Plant Physiol. 2008 EFFECT OF LIGHT QUALITY ON FLAVONOID BIOSYNTHESIS IN BILBERRY (VACCINIUM MYRTILLUS) Laura Zoratti, Marko Suokas, Marian Sarala, Hely Häggman, Laura Jaakola Department of Biology, University of Oulu, Oulu, Finland E-mail: Laura.Zoratti@oulu.fi 83 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Flavonoids are plant secondary metabolites well known for their high antioxidant activity, which is an important property for plants´ interaction and adaptation with the surrounding environment. As well, they contribute to the organoleptic quality of plant products, like fruits, and are of major interest in human nutrition and health. Bilberry is a characteristic berry species of boreal forests and naturally contains high amounts of flavonoids, in particular anthocyanins. In bilberry, the synthesis and accumulation of these compounds is genetically, developmentally, and also environmentally regulated. Generally, our bilberry research is focused on regulation of flavonoid biosynthesis related to adaptation. In particular, we are studying the role of light quality on the production of flavonoids. Results show considerable differences in the expression of structural and regulatory flavonoid biosynthesis genes, as well as the photoreceptor genes PHYTOCHROME B and CRYPTOCHROME, in response to different light treatments. CHANGING THE BIOMASS COMPOSITION OF BRACHYPODIUM DISTACHYON -MORE FRUCTANS FOR A MORE EFFICIENT BIOMASS CONVERSION Maria Lundmark, Helle Kildal Mogensen, Tom Hamborg Nielsen Department of Plant Biology and Biotechnology, University of Copenhagen, Copenhagen, Denmark E-mail: mlun@life.ku.dk Fructans are non-structural polysaccharides consisting of either β -2,1- or β -2,6-linked linear and branched fructose chains that act as a carbohydrate reserves as an alternative or in addition to starch. Fructans are commonly found in many of the important biofuel crops such as Switchgrass, Perennial ryegrass and Miscanthus. Plant biomass is the largest available resource for the production of renewable liquid fuels. However, the conversion of lignocelluloic biomass into biofuel is difficult due to the recalcitrance of these structures and the efficiency in terms of yield and cost is still too poor for it to be feasible at a commercial level. We intend to shift the carbon flux of the photosynthetic cells of Brachypodium distachyon towards more soluble carbohydrates by overexpressing the enzymes of the fructan biosynthetic pathway. The shift in biomass composition will allow for more efficient degradation. We have identified three putative fructosyl transferases from Brachypodium. These have been cloned and we are waiting for the first generation of the transgenic plants. Alongside, we have also cloned genes encoding fructan exohydrolases in Brachypodium and an endohydrolase from Bacillus subtilis, in order to obtain new enzymes suitable for degrading fructans stored within the leaves. CLONING, CHARACTERIZATION AND EXPRESSION PROFILES OF TWO UDP- GLUCOSYLTRANSFERASES, UGT85K4 AND UGT85K5, RESPONSIBLE FOR THE LAST STEP IN THE CYANOGENIC GLUCOSIDE BIOSYNTHETIC PATHWAY IN CASSAVA Rubini Kannangara 1 , Mohammed Saddik Motawia 1 , Natascha Kristine Krahl Hansen 1 , Suzanne Michelle Paquette 2 , Birger Lindberg Møller 1 , Kirsten Jørgensen 1 1 Department of Plant Biology and Biotechnology, Villum Foundation Research Centre “Pro-Active Plants”, UNIK Center for Synthetic Biology, University of Copenhagen, Copenhagen, Denmark. 2 Department of Biological Structure, University of Washington, Seattle, WA, USA E-mail: natascha@life.ku.dk Cyanogenic glucosides are amino acid-derived bioactive natural products biosynthesized by two cytochrome P450 enzymes and an UDP-glucosyl transferase. Cassava (Manihot esculenta) biosynthesizes the two cyanogenic glucosides, linamarin and lotaustralin, derived from L-valine and L-isoleucine respectively. In this study cDNAs encoding two UGT paralogs (assigned UGT85K4 and UGT85K5) have been identified and isolated from cassava. The two paralogs show 96 % identity and belong to a family containing UGTs involved in cyanogenic glucoside biosynthesis in Prunus dulcis and Sorghum bicolor. The two UGT paralogs were recombinantly expressed in Escherichia coli and biochemically characterized. UGT85K4 and 84 X X I V S P P S C O N G R E S S 2 0 1 1
Page 1 and 2:
��
Page 3 and 4:
Foreword eword ord A B S T R A C T
Page 5 and 6:
Program of the Congress gram of the
Page 7 and 8:
A B S T R A C T B O O K - P R O G R
Page 9 and 10:
A B S T R A C T B O O K - P R O G R
Page 11 and 12:
Plenary session 1 A B S T R A C T B
Page 13 and 14:
Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16:
Session 1. Biotechnology and synthe
Page 17 and 18:
Session 1. Biotechnology and synthe
Page 19 and 20:
Session 2. Systems biology and -omi
Page 21 and 22:
Session 2. Systems biology and -omi
Page 23 and 24:
A B S T R A C T B O O K - A B S T R
Page 25 and 26:
A B S T R A C T B O O K - A B S T R
Page 27 and 28:
Pleanary session 4 A B S T R A C T
Page 29 and 30:
Invited talk 4 A B S T R A C T B O
Page 31 and 32:
Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 43 and 44: A B S T R A C T B O O K - A B S T R
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
Page 67 and 68: Sapporo Medical University, Sapporo
Page 83: A B S T R A C T B O O K - A B S T R
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
show all

1. Front Cover.cdr - CORE

Create successful ePaper yourself

Delete template?

Save as template?