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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Ca. In Al-treated roots, NR activity was increased and IAA concentration was maintained at the same level as pretreatment, and indole-3-acetyl-L-aspartic acid (IAAsp), which is a metabolic intermediate of IAA degradation, was not detected in roots. In Ca-treated roots, NR activity increased, but IAA concentration decreased as IAAsp increased. Thus, the maintenance of IAA concentration in Al-treated roots seemed to suppress the process of IAA decomposition. Al treatment increased the length and number of second lateral roots but Ca treatment did not. We concluded that root development induced by Al was related to NR activity and maintenance of IAA concentration in roots. PHYSIOLOGICAL AND GENETIC CHARACTERIZATION OF NOVEL INDOLE-3-BUTYRIC ACID RESISTANT MUTANTS Taiki Hanzawa 1 , Gloria Muday 2 , Abidur Rahman 1 1 Cryobiofrontier Research Center, Iwate University, Morioka, Japan 2 Wake Forest University, Winston-Salem, USA E-mail. a2511008@iwate-u.ac.jp Although two predominant forms of endogenous auxins, IAA, and IBA are produced by the plant, the majority of the research to date has been focused on IAA. The conversion of IBA to IAA via beta-oxidation predicts that IBA may act solely via IAA pathways. However, IBA has been shown to have more potent activity than IAA in some physiological responses. Further, it has been recently reported that the loss of IAA uptake carrier or efflux carrier does not affect the intracellular IBA transport. These results suggest that IBA transport and signaling pathways may differ from those used by IAA. However, the molecular mechanism of IBA specific transport or signaling pathways remains elusive. In the present study, we tried to identify IBA specific signaling by screening for novel IBA-resistant mutants. From this screen we recovered three mutant lines, which show only a specific resistance to IBA induced root growth inhibition and lateral root formation, but respond to other growth regulators, including IAA, exactly as wild type. All three mutants showed reduced transport activity for IBA, but not IAA, indicating that the mutated genes may regulate the IBA specific transport process. THE ROLE OF GDSL-LIPASES IN ARABIDOPSIS PETAL DEVELOPMENT AND FUNCTION Tamar Rosilio-Brami, Michele Zaccai, Moriyah Zik Life Sciences Department, Ben-Gurion University of the Negev, Beer Sheva, Israel E-mail: tamarosilio@gmail.com The GDSL-lipase gene family encodes for hydrolytic enzymes named after a unique sequence of amino acids, the GDSL motif found near the N-terminus of the protein. GDSL-lipases have a flexible active site that enables broad substrate specificity and facilitates different enzymatic activities, including lipase, esterase and protease activities. Several microarray studies, aimed at identifying genes involved in the development of floral organs, demonstrated that members of this gene family are significantly and differentially expressed in these organs, particularly in petals. Thus far the in planta function of only a few GDSL-lipases have been demonstrated. In order to elucidate GDSL-lipases unique and common roles in petals, we initiated a detailed study of the expression patterns of six Arabidopsis GDSL-lipase genes which are abundantly expressed in petals. Possible functions of these genes in planta can be drawn from the close correlation between their spatial and temporal expression and physiological events that take place throughout flower development. The expression of the six studied genes is mainly associated with physiological events related to anthesis and senescence that involve lipid metabolism processes, plant defense pathways and cuticule metabolism. In parallel to the expression pattern analysis, we are conducting phenotypic analyses of petal GDSL-lipases mutant lines. THE USE OF THE 7B-1 AND CRY1-1 MUTANTS UNRAVELED A SPECIFIC INVOLVEMENT OF IP DURING DE-ETIOLATION IN TOMATO (SOLANUM LYCOPERSICUM L.) 67 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Véronique Bergougnoux 1 , David Zalabák 2 , Hana Pospíšilová, Michaela Jandová 3 , Ondřej Novák 1 , Martin Fellner 1 1 Palacky University in Olomouc and IEB ASCR, Laboratory of Growth Regulators, Olomouc, Czech Republic 2 Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Molecular Biology, Faculty of Science, Palacky University, Olomouc, Czech Republic 3 Department of Botany, Palacky University in Olomouc, Faculty of Science, Olomouc, Czech Republic E-mail: v.bergougnoux @yahoo.fr As sessile organisms, plants have evolved sophisticated mechanisms to adapt to environmental conditions. Light is one of the most important factor influencing plant growth and development all through their life cycle. One of the well known light-regulated processes is de-etiolation, i.e. the switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an important role during de-etiolation as high concentration of CKs in the culture medium induced photomorphogenesis in dark-grown seedlings. Using two tomato mutants deficient in blue light (BL)-induced responses and their corresponding wild-types (WT), de-etiolation in tomato was investigated. Under BL, the two mutants had longer hypocotyls than their corresponding WT. This was correlated with longer epidermal cells, higher proportion of epidermal cells with high ploidy and lower osmotic pressure in hypocotyl. The changes in endogenous CK were measured after exposure to BL and the cytokinin iP was found to be specifically involved in the BL-induced de-etiolation. We revealed also that BL modulates expression of genes encoding proteins involved in the iP metabolism. To our knowledge, this study unravels for the first time the specific role of iP in BL-induced de-etiolation. Acknowledgements This work was supported by grant MSM6198959215 (MšMT) and P501/10/0785 INTER-ORGANELLAR H2O2 SIGNALING IN ARABIDOPSIS Altynai Adilbayeva, Jodi Maple, Sigrun Reumann, Simon Geir Møller Centre for Organelle Research, University of Stavanger, Stavanger, Norway E-mail:altynai.adilbayeva@uis.no Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) are highly toxic molecules that are daily produced as by-products of normal metabolism and at increased rate under biotic and abiotic stress conditions. Apart from their toxic function, ROS also play essential roles as second messengers in several signal transduction cascades, ultimately inducing the expression of defence and adaptation genes and allowing organisms to tolerate adverse conditions. The ROS signaling network is highly complex and dynamic due to compartmentalization of both ROS production and scavenging at multiple sites. We aim at studying cellular H2O2 dynamics by an integrative approach considering several major cell compartments. Proteinaceous ROS sensors and spectral variants of green fluorescent protein are targeted to different cell compartments (e.g., chloroplasts, mitochondria, peroxisomes) to create multiply labeled Arabidopsis cell lines for integrated, temporal and spatial high-resolution analysis of H2O2 dynamics by confocal microscopy (Nikon A1R). ROS homeostasis will be perturbed by various means (e.g., abiotic stress) in wild-type plants and gain- and loss-of-function mutants of antioxidative enzymes and major signal transduction components. Cutting edge imaging technologies will be applied to comprehensively dissect abiotic H2O2 signaling and accurate monitoring of H2O2 production, diffusion and scavenging by a non-invasive method. NIGHT-TIME PR:PFR RATIO AND DAYTIME STOMATAL OZONE UPTAKE Ane V. Vollsnes 1 , Aud B. Eriksen 1 , Cecilia M. Futsaether 2 , Ole Mathis Opstad Kruse 2 1 University of Oslo, Oslo, Norway 2 Norwegian University of Life Sciences, Ås, Norway E-mail: a.v.vollsnes@bio.uio.no In Trifolium subterraneum, oxidative stress due to ozone has been shown to result in more severe visible foliar injuries when phytochrome is predominantely in the far-red absorbing conformation during night than when it is in the red absorbing conformation. Phytochrome may be involved in stomata opening in the 68 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
Page 17 and 18: Session 1. Biotechnology and synthe
Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
Page 23 and 24: A B S T R A C T B O O K - A B S T R
Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
Page 67: Sapporo Medical University, Sapporo
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
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1. Front Cover.cdr - CORE

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