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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S Research Centre Flakkebjerg, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark E-mail: Remy.Kronbak@agrsci.dk In Arabidopsis specifically mutated resistance genes (R genes) act dominant negatively conferring susceptibility, when introduced into plants resistant to the considered pathogen due to the presence of the wildtype R gene. Based on this, a strategy to match R genes to specific pathogens has been designed in wheat. Proof-of-concept will be established in the wheat cultivar ‘Bobwhite S-26’ using the wheat yellow rust system. The yellow rust R gene Yr10 will be transformed into ‘Bobwhite S-26’, and transgenic plants will be tested for achieved resistance against an appropriate avirulent P. striiformis isolate harboring the AvrYr10 gene. Plants transformed with both the native and the mutated Yr10 forms will prove the concept if they show susceptibility in this pathogen test. For the screening strategy, a ‘Bobwhite S-26’-specific library of expressed NB-LRR type R genes will be generated from deep transcriptome sequencing. Transgenic Bobwhite lines with mutated forms of R genes will be inoculated with selected avirulent isolates of P. striiformis. Susceptibility will indicate that a mutated form of the R gene that confers resistance to a particular isolate is present in the transgenic plant, and in this way the R gene can be identified and exploited in breeding for disease resistance. IS JASMONATE-REGULATED DEFENCE EFFICIENT AGAINST BIRD CHERRY-OAT APHID (RHOPALOSIPHUM PADI)? Sara Merhabi, Inger Åhman, Lisa Beste, Lisbeth Jonsson Botany Department, Stockholm University, Stockholm, Sweden E-mail: mehrabi@botan.su.se The bird cherry -oat aphid (Rhopalosiphum padi) is a cereal pest worldwide. The aphid causes no visible symptoms but reduces the yield at heavy infestations and is a vector of plant viruses. An accession of wild barley (Hordeum vulgare ssp. spontaneum) exhibited antibiosis against R. padi and was crossed with modern barley cultivars. Further breeding has resulted in a number of doubled-haploid breeding lines with partial resistance to R. padi. It has been suggested that efficient plant defence against phloem-feeding insects is regulated by jasmonate but that the insects are able to suppress or avoid this defence [1]. In microarray studies with two R. padi-resistant and two susceptible barley genotypes, methyl jasmonate (MeJA)-responsive genes did not appear as a category that was differentially regulated by aphids. Some MeJA-responsive gene sequences did however exhibit constitutive strong expression specifically in the two aphid-resistant genotypes. This prompted further investigation. We now report the constitutive gene expression of a selection of MeJA-regulated genes in a number of barley genotypes with known levels of aphid resistance. They represent the parents used in the crosses between wild barley and modern cultivars, their offspring and offspring from first and second generation of back-crosses. References [1] Walling, Plant Physiol. 2008 UPTAKE AND TRANSLOCATION OF XENOBIOTICS IN CEREALS AND TURNIP RAPESEEDS Trine Eggen, Cathrine Lillo Bioforsk Vest Særheim, Klepp Stasjon. Norway E-mail: Trine.Eggen@bioforsk.no Uptake and translocation of xenobiotics from soil to seeds of barley (Hordeum vulgare cv. Edel), wheat (Triticumaestivum cv. Bjarne), oat (Avena sativa cv. Berlinda) and turnip rape (Brassica rapa, var. oleifera L. cv. Valo, Brassica napus cv. Sheik) were investigated. The studied compounds were emerging organic compounds with potential transfer from environment to terrestrial human food chain via application of sewage sludge on agricultural soil. The plant uptake study was performed as pot experiment in greenhouse with agricultural soil (0.7 % organic carbon) spiked with xenobiotics. High variations of uptake and translocation of different xenobiotics, and between plant species were observed; even bioaccumulation in seeds were measured. Expressed as a seed concentration factor 89 X X I V S P P S C O N G R E S S 2 0 1 1
SCF = A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S values in the range of 1-18 was measured. 90 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
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Pleanary session 4 A B S T R A C T
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Invited talk 4 A B S T R A C T B O
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Session 5.Cell biology A B S T R A
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Session 6.Organelle biology A B S T
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Session 6.Organelle biology A B S T
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Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
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