1. Front Cover.cdr - CORE

Recommendations

Info

A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S morning, which could lead to differences in subsequent ozone uptake and consequently differences in visible foliar injuries. This hypothesis was tested by studying leaf temperature measurements obtained noninvasively through thermography. Leaf temperature is strongly dependent on transpiration, which in turn depends on stomata opening. Plants kept in darkness during night were compared to plants kept in dim farred, red or white light during night. Half the plants were exposed to ozone on three consecutive days. The main finding was that morning leaf temperatures were the same regardless of night-time illumination. Thus, all plants had the same potential for ozone uptake before ozone exposure started. On the other hand, evening leaf temperatures, shortly after the end of an ozone exposure period, were affected by an interaction between ozone exposure and night light conditions. MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN ROBINIA PSEUDOACACIA LEAFLETS D. Vidal, Q. Wang, E. Simón Plant Physiology Department, Faculty of Biology, University of Barcelona, Barcelona, Spain E-mail: dvidal@ub.edu Mitogen-activated protein kinases (MAPKs) are involved in the signal transduction of plant responses to environmental stimuli. Protein phosphorylation by MAPKs is typically organized in signalling cascades. It is estimated that about 10 % of all plant kinases (PKs) are involved in MAPK pathways. We previously reported MAPK activity, affected by red (R) and far-red (FR) light, in cucumber cotyledons. Here we examine whether R. pseudoacacia leaflets show MAPK activity. By performing immunodetection assays with polyclonal antibodies directed against mammalian ERK1/2 and in-gel phosphorylation assays, a MAPK-like activity was detected in Robinia leaflet extracts. Immunoprecipitation with antibodies anti-pERK (Tyr 204), anti-pERK1/2 and anti-ERK1/2 was used to purify putative MAPKs from the extracts. Two proteins (of about 53 y 50 kDa) were recognised by the antibodies, indicating that they had immunological characteristics of MAPKs. Both proteins were also recognised in the immunoblotting assays and detected by the chemiluminescent system. The in-gel phosphorylation assays using SDS-PAGE polymerized with myelin basic protein (MBP) as exogenous substrate and [γ-32P] ATP, as phosphate donor, showed that the proteins of 53 kDa and 50 kDa had MAPK-like activity, although the PK activity of the smaller protein was only scarcely detected. REDOX INSENSITIVE2, A NOVEL CHLOROPLAST PROTEIN LINKING REDOX REGULATION OF PHANG EXPRESSION TO PEP ACTIVITY IN THE CHLOROPLASTS Peter Kindgren, Juan de Dios Barajas López, Dmitry Kremnev, Aurora Piñas Fernández, Christian Tellgren- Roth, Ian Small, Åsa Strand Umeå Plant Science Centre, Umeå, Sweden E-mail: dmitry.kremnev@plantphys.umu.se The photosynthetic apparatus is composed of proteins encoded in nuclear and chloroplastic genomes and their activities are coordinated through intracellular signaling. The plastids produce multiple retrograde signals throughout development and in response to changes in the environment. These signals regulate the expression of photosynthesis-associated nuclear genes (PhANGs). Using forward genetics we identified REDOX INSENSITIVE2 (RIN2), a novel chloroplast component involved in redox-mediated retrograde signaling. The allelic mutants rin2-1 and rin2-2 demonstrated a mis-regulation of PhANG expression in response to excess light and inhibition of photosynthetic electron transport. As a consequence of the misregulation of PhANGs, the rin2 mutants displayed a high irradiance-sensitive phenotype with significant photoinactivation of PSII. RIN2 is localized to the nucleoids and plastid transcriptome analyses demonstrated that RIN2 is required for full expression of genes transcribed by the plastid-encoded RNA Polymerase (PEP). A direct role for PEP activity in redox-mediated retrograde signaling. Taken together, our results indicate that RIN2 is part of the PEP machinery and that the PEP complex responds to photosynthetic electron transport and generates a retrograde signal enabling the plant to synchronize the expression of photosynthetic genes from both the nuclear and plastidic genomes. 69 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S INVOLVEMENT OF CGMP IN THE NITRIC OXIDE EFFECT ON THE PHYTOCHROME- MEDIATED NYCTINASTIC CLOSURE OF ALBIZIA LOPHANTHA LEAFLETS S. Chellik, C. Bergareche, L. Moysset, E. Simón Plant Physiology Department, Faculty of Biology, University of Barcelona, Barcelona, Spain E-mail: esimon@ub.edu Albizia lophantha leaflets show both rhythmic and nyctinastic movements, from a horizontally extended position in daylight to a folded position at night. These movements are under phytochrome control, through a low fluence response. Leaflet movements depend on the curvature of a specialized motor organ, the pulvinus, located at the base of leaflets. Pulvinar curvature is caused by turgor changes in both extensor and flexor pulvinar motor cells, which in turn are driven by K + and Cl - ionic fluxes. Nitric oxide (NO) is a cellular signalling molecule which affects the activity of ionic channels. Previous data indicate that NO inhibits nyctinastic closure and this inhibition it is more apparent after red-light irradiation. Here we examine whether the NO effect is associated with changes in cGMP, by testing inhibitors of guanylate cyclase (ODQ and Ly85.583) and phosphodiesterase (sildenafil) as well as an analogue of cGMP (8-BrcGMP). Exogenous donors of NO inhibited nyctinastic closure, but simultaneous application of ODQ cancelled this inhibitory effect. ODQ (25-200 µM) and Ly85.583 (50 µM) enhanced nyctinastic closure. Sildenafil and 8-Br-cGMP inhibited nyctinastic closure. All these data implicate cGMP is involved in the NO effect on phytochrome-mediated nyctinastic closure. PROTEIN PHOSPHATSE 2A REGULATORY SUBUNITS ARE ESSENTIAL FOR METABOLISM AND PLANT DEVELOPMENT Behzad Heidari, Polina Matre, Else Müller Jonassen, Dugassa Nemie-Feyissa, Christian Meyer, Odd Arne Rognli, Simon G. Møller, Cathrine Lillo Center for Organelle Research, University of Stavanger, Stavanger, Norway E-mail: behzad.hidary@uis.no, cathrine.lillo@uis.no Canonical protein phosphatases 2A (PP2A) are trimeric protein complexes present in all eukaryotes cells, and known to be involved in regulation of cell cycle, hormone signaling and stress response. PP2A consists of a catalytic (C), scaffolding (A) and regulatory (B) subunit. The B subunits are divided into three (nonrelated) groups B55, B' and B''. The B subunits are generally thought to confer substrate specificity and cellular localization of the PP2A complex, hence providing for the specific functions of different PP2As. Bimolecular fluorescence complementation (BiFC) was used to identify PP2A regulatory subunits interacting with nitrate reductase (NR), and the two B55 (α and β) subunits were found to be positive. Dosage test of B55 effect on NR activation revealed that B55 promoted activation of NR. Interestingly, the homozygous double mutant (b55αβ) appeared to be lethal, which shows that the B55 group has essential functions that cannot be replaced by other regulatory B subunits. The B'α subfamily consists of two members B'α and B'β. A double mutant b'αβ null in B'α, but 1% of wild type B'β transcript levels showed a striking phenotype with poor seed set, pointing also to the B'α subfamily as being necessary for survival of the plant. THE ROLE OF NONSPECIFIC PHOSPHOLIPASE C IN PLANT STRESS RESPONSES Daniela Kocourkova, Premysl Pejchar, Zuzana Krckova, Olga Valentova, Jan Martinec Institute of Experimental Botany, ASCR, Prague, Czech Republic E-mail: martinec@ueb.cas.cz Phospholipid signalling is one of the key cellular regulatory mechanisms in plants in which phospholipases play a central role. A novel member of plant phospholipid signalling, nonspecific-phospholipases C (NPC) has been recognized only recently. Molecular, cellular and functional characterization of the small NPC gene family is still in its infancy. The gene family in Arabidopsis consists of six genes NPC1-NPC6. Among them, only NPC4 and NPC5 were heterologously expressed and partially characterized. Almost nothing is known about mechanism of NPC activation and downstream processes. 70 X X I V S P P S C O N G R E S S 2 0 1 1
Page 1 and 2:
��
Page 3 and 4:
Foreword eword ord A B S T R A C T
Page 5 and 6:
Program of the Congress gram of the
Page 7 and 8:
A B S T R A C T B O O K - P R O G R
Page 9 and 10:
A B S T R A C T B O O K - P R O G R
Page 11 and 12:
Plenary session 1 A B S T R A C T B
Page 13 and 14:
Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16:
Session 1. Biotechnology and synthe
Page 17 and 18:
Session 1. Biotechnology and synthe
Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
Page 23 and 24: A B S T R A C T B O O K - A B S T R
Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
Page 67 and 68: Sapporo Medical University, Sapporo
Page 69: A B S T R A C T B O O K - A B S T R
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
show all

1. Front Cover.cdr - CORE

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?