1. Front Cover.cdr - CORE

A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S
Ca. In Al-treated roots, NR activity was increased and IAA concentration was maintained at the same level
as pretreatment, and indole-3-acetyl-L-aspartic acid (IAAsp), which is a metabolic intermediate of IAA
degradation, was not detected in roots. In Ca-treated roots, NR activity increased, but IAA concentration
decreased as IAAsp increased. Thus, the maintenance of IAA concentration in Al-treated roots seemed to
suppress the process of IAA decomposition. Al treatment increased the length and number of second
lateral roots but Ca treatment did not. We concluded that root development induced by Al was related to
NR activity and maintenance of IAA concentration in roots.
PHYSIOLOGICAL AND GENETIC CHARACTERIZATION OF NOVEL INDOLE-3-BUTYRIC ACID
RESISTANT MUTANTS
Taiki Hanzawa 1 , Gloria Muday 2 , Abidur Rahman 1
1 Cryobiofrontier Research Center, Iwate University, Morioka, Japan
2 Wake Forest University, Winston-Salem, USA
E-mail. a2511008@iwate-u.ac.jp
Although two predominant forms of endogenous auxins, IAA, and IBA are produced by the plant, the
majority of the research to date has been focused on IAA. The conversion of IBA to IAA via beta-oxidation
predicts that IBA may act solely via IAA pathways. However, IBA has been shown to have more potent
activity than IAA in some physiological responses. Further, it has been recently reported that the loss of IAA
uptake carrier or efflux carrier does not affect the intracellular IBA transport. These results suggest that IBA
transport and signaling pathways may differ from those used by IAA. However, the molecular mechanism
of IBA specific transport or signaling pathways remains elusive. In the present study, we tried to identify
IBA specific signaling by screening for novel IBA-resistant mutants. From this screen we recovered three
mutant lines, which show only a specific resistance to IBA induced root growth inhibition and lateral root
formation, but respond to other growth regulators, including IAA, exactly as wild type. All three mutants
showed reduced transport activity for IBA, but not IAA, indicating that the mutated genes may regulate the
IBA specific transport process.
THE ROLE OF GDSL-LIPASES IN ARABIDOPSIS PETAL DEVELOPMENT AND FUNCTION
Tamar Rosilio-Brami, Michele Zaccai, Moriyah Zik
Life Sciences Department, Ben-Gurion University of the Negev, Beer Sheva, Israel
E-mail: tamarosilio@gmail.com
The GDSL-lipase gene family encodes for hydrolytic enzymes named after a unique sequence of amino
acids, the GDSL motif found near the N-terminus of the protein. GDSL-lipases have a flexible active site that
enables broad substrate specificity and facilitates different enzymatic activities, including lipase, esterase
and protease activities. Several microarray studies, aimed at identifying genes involved in the development
of floral organs, demonstrated that members of this gene family are significantly and differentially
expressed in these organs, particularly in petals. Thus far the in planta function of only a few GDSL-lipases
have been demonstrated. In order to elucidate GDSL-lipases unique and common roles in petals, we
initiated a detailed study of the expression patterns of six Arabidopsis GDSL-lipase genes which are
abundantly expressed in petals. Possible functions of these genes in planta can be drawn from the close
correlation between their spatial and temporal expression and physiological events that take place
throughout flower development. The expression of the six studied genes is mainly associated with
physiological events related to anthesis and senescence that involve lipid metabolism processes, plant
defense pathways and cuticule metabolism. In parallel to the expression pattern analysis, we are
conducting phenotypic analyses of petal GDSL-lipases mutant lines.
THE USE OF THE 7B-1 AND CRY1-1 MUTANTS UNRAVELED A SPECIFIC INVOLVEMENT OF
IP DURING DE-ETIOLATION IN TOMATO (SOLANUM LYCOPERSICUM L.)
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