1. Front Cover.cdr - CORE
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A B S T R A C T B O O K – A B S T R A C T S O F T A L K S<br />
51<br />
X X I V S P P S C O N G R E S S 2 0 1 1<br />
Session 13.Pathogen defense and plant desease<br />
PSEUDOMONAS SYRINGAE PV.PHASEOLICOLA AND DICKEYA DADANTII 3937:<br />
TWO PATHOGENIC LIFE-STYLES ANALYSED BY IMAGING TECHNIQUES<br />
M. Luisa Pérez-Bueno 1 , Emilia López-Solanilla 2 , Mónica Pineda 1 , Elena Díaz Casado, Cayo<br />
Ramos 3 , Pablo Rodríguez-Palenzuela 2 , Matilde Barón 1<br />
1 Estación Experimental del Zaidín, CSIC, Granada, Spain<br />
2 Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid - Instituto Nacional de Investigación<br />
y Tecnología Agraria y Alimentaria, Madrid, Spain<br />
3 Area of Genetics, University of Malaga, Malaga, Spain<br />
E-mail: marisa.perez@eez.csic.es<br />
P. syringaepv. phaseolicola (Pph) and D. dadantii (D.d.) 3937 are model organisms for two<br />
very different “pathogenic life-styles”. The first one is the causal agent of halo blight<br />
disease of beans, characterized by small watersoaked spots which gradually turn<br />
necrotic, and become surrounded by a wide chlorotic halo (caused by the effect of a nonhost<br />
specific toxin produced by the bacteria). The second one is the causal agent of<br />
bacterial soft rot in vegetables. This disease occurs most commonly on fleshy storage<br />
tissues. Both pathogens cause great losses in crop production worldwide.<br />
Chlorophyll fluorescence (Chl-F) and blue-green fluorescence (BGF) techniques are<br />
sensitive and specific tools which provide information about the primary and secondary<br />
metabolism in plants, respectively. Chl-F, BGF and temperature leaf patterns obtained by<br />
imaging techniques were shown to be in correspondence with pathogen distribution and<br />
symptom development during viral pathogenesis. In the present work, the systemic<br />
infection of D.d. 3937 in N. benthamiana and Pph in Phaseolus vulgaris have been studied<br />
by these imaging techniques to understand the impact of these pathogens in plant<br />
physiology.<br />
Acknowledgements<br />
Financial support: MCINN-FEDER/AGL2008-00214, CVI 03475 (Junta de Andalucía). MLPB was recipient of a<br />
CSIC-JAE Doc contract.