1. Front Cover.cdr - CORE

A B S T R A C T B O O K – A B S T R A C T S O F T A L K S
CHLOROPLAST DIFFERENTIATION BY SECRETORY PROTEIN
Yasuo Niwa, Akiko Ogino, Hironori Kageshima, Shingo Goto, Hirokazu Kobayashi
University of Shizuoka, Shizuoka, Japan
E-mail: niwa@u-shizuoka-ken.ac.jp
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Session 5.Cell biology
The plant hormones such as auxin and cytokinin can control plant cell de-differentiation
and re-differentiation. It is possible to induce green callus from white callus by high
concentration of cytokinin treatment. In this study, white callus induced from roots of
Arabidopsis were used for mutant screening by activation tagging. Mutant candidates
were selected as a greening phenotype from the white callus without high levels of
cytokinin treatment. As a result, three candidates can be obtained and named ces101, 102,
and 103. To identify the gene for ces102 phenotype, TAIL-PCR (thermal asymmetric
interlaced-PCR) was performed. T-DNAs were found to be inserted into two locations in
Arabidopsis genome. The expression level of two genes located near the insertion points
was increased. From the results of phenotypic analysis by over-expression of candidate
genes, the gene encoding 119 amino acids corresponds to ces102. According to the
characteristics of the amino acid sequence, CES102 was expected to have a signal
peptide. Localization analysis of GFP fusion protein, CES102-GFP could be detected at ER.
From these results, plastid differentiation might be controlled through the secretory
pathway.