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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S INCREASING PROTEIN QUALITY/QUANTITY AND ZN CONTENT OF BARLEY GRAIN Mohammad Nasir Uddin 1 , Mette Lange 3 , Jan K. Schjørring 2 , Preben Bach Holm 1 , Eva Vincze 2 1 University of Aarhus, Aarhus, Denmark 2 University of Copenhagen, Copenhagen, Denmark 3 University of Aalborg, Aalborg, Denmark E-mail: MohammadNasir.Uddin@agrsci.dk Atmospheric CO2 enrichment may lead to increased yields of cereal species but there will be a “dilution” of other nutrients like minerals and proteins in the grain. The nutritional short-comings can not be met solely by using higher amounts of nitrogen and mineral fertilizer since this would increase nitrogen and mineral loads into the environment. Therefore, alternative approaches embracing creation of transgenic lines or exploitation of the natural variation are needed to increase grain Zn content. In our experiments, transgenic barley lines with a reduced C-hordein content showed not only an altered amino acid composition but also a 2-3 fold increase in the grain Zn concentration. From bioinformatic studies we have identified protein families which potentially may bind more Zn. The expression levels of the selected gene/protein families are currently being assessed by quantitative PCR. We also have a collection of close to 300 barley cultivars with high grain protein content and a substantial genetic variation in grain Zn concentration. We will use them to further explore the potential link between protein quality/quantity and Zn content under elevated atmospheric CO2 level. ANALYSIS OF CARBON ION BEAM INDUCED UVB TOLERANT AND SENSITIVE RICE MUTANTS Nao Takano 1 , Yuko Takahashi 1 , Misturu Yamamoto 1 , Mika Teranishi 1 , Yoshihiro Hase 2 , Ayako Sakamoto 2 , Atsushi Tanaka 2 , Jun Hidema 1 1 Graduate School of Life Sciences, Tohoku University, Sendai, Tōhoku, Japan 2 Radiation-Applied Biology Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Ibaraki Prefecture, Tokaimura, Japan E-mail:n-takano@ige.tohoku.ac.jp UVB radiation can damage plant growth and development. To develop the factors related to UVB resistance, Japanese rice cultivar “Sasanishiki” was exposed to carbon ion beam (320 MeV: 12C6+, 80 Gy), which have been used to generate new mutants varieties, and two UVB tolerant and three UVB sensitive mutants were isolated. We focused on UVB tolerant mutant utr-319 (UV Tolerant Rice-319) and UVB sensitive mutant usr-1 (UV Sensitive Rice-1), because the levels of UV-absorbing compounds or CPD photorepair activity, which has been thought as factors related UVB tolerance of each mutant wasn't affected compared to wild-type. First, to estimate the mutated regions of each mutant, we performed Array Comparative Genomic Hybridization, and then, genomic DNA sequence analysis using BAC clone of each mutant. As a result, we found that approximately 40 kb on chromosome 7 containing 2 genes (AK111251, AK071492: function of each gene product has not been unknown) was deleted in utr-319. In usr-1 mutant, we found that approximately 53 kb on chromosome 7 containing 2 genes (AK073834; encodes thioesterase domain, AK121807; encodes Rpb6) was deleted. In this meeting, we introduce the characters of utr-319 and usr-1, and discuss about relationships between deleted genes and UVB tolerance or sensitivity. THE INTERACTION BETWEEN DEVELOPMENT AND STRESS IN THE LOW TEMPERATURE METABOLOME OF ARABIDOPSIS THALIANA Paulina Stachula, M. Lundmark, L. Hendrickson, E. Pesquet, C. Külheim, A. Vlčková, H. Stenlund, T. Moritz, Å. Strand, V. Hurry Umeå Plant Science Centre, Umeå, Sweden E-mail: paulina.stachula@plantphys.umu.se 79 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S In an attempt to discriminate between a low temperature stress versus cold acclimation responses we contrasted the anatomical, physiological and metabolic profile changes of Arabidopsis thaliana plants grown under different temperature regimes. We contrasted the characteristics of 4-week old 23°Cgrown plants that were subsequently exposed to 5°C and allowed to develop new mature leaves (CD) to plants that germinated and spent their life cycle in 5°C (CG). For most parameters measured, the CG phenotype exhibited intermediate characteristics between WG and CD plants. This result was accompanied by improved photosynthetic capacity and daily photosynthetic carbon gain of CG relative to CD plants when considering primary resource investment per leaf. The difference between cold stress and development (CD) versus cold growth (CG) was further demonstrated by the fact that the CD and CG leaves had equal freezing tolerance yet metabolically the CG plants were more similar to WG leaves. Thus, these experiments demonstrate that if we are to develop an more informative and integrative approach to understand abiotic stress acclimation responses it is important to find experimental systems where we can begin to filter the large number of changes in gene expression, protein and metabolite abundance that occur in response to the imposed stress, but are not acclimative, in order to determine those, that are a direct response leading to acclimation. GROWTH AND METABOLIC ACCLIMATION OF BETULA PENDULA TO DIFFERENT NIGHT- TIME TEMPERATURES Sari Kontunen-Soppela, Maarit Mäenpää, Vladimir Ossipov, Markku Keinänen, Matti Rousi, Elina Oksanen University of Eastern Finland, Joensuu, Kuopio and Savonlinna, Finland E-mail: sari.kontunen-soppela@uef.fi The climatic change with increasing average air temperature, but without a change in photoperiod, poses plants to a new combination of light and temperature. Night temperatures in northern latitudes are expected to increase more than the day temperatures. Three genotypes of silver birch (B. pendula) were exposed to same daytime temperature (22°C), but to five different night-time temperatures (6, 10, 14, 18, 22°C) in growth chambers. Photoperiod was 14h full light with gradually increasing morning (2h) and decreasing evening (2h) light. Gas exchange, growth and the concentrations of ca. 280 metabolites were measured. Warmer night temperatures increased dark-respiration, but no effects were seen in daytime CO2 assimilation. In contrast, stomatal conductance increased at 22°C during daytime, but no changes were observed at night. The height growth of birches was greater at warmer temperatures; total biomass was, however, not affected. Genotype was the strongest factor for determining metabolic composition of the plants. Genotypes had both unique and shared metabolic responses to night temperatures. The responses common for all genotypes were connected to growth and the developmental state of plants. Changes in growth and metabolite concentrations were non-linear along the temperature gradient and the sensitivity to react and the critical temperatures were genotype-specific. TRANSCRIPTIONAL REGULATION OF THE COLD-INDUCIBLE DREB1 GENES IN ARABIDOPSIS Satoshi Kidokoro, Kyonoshin Maruyama, Nobutaka Mitsuda, Masaru Ohme-Takagi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan E-mail: akido@mail.ecc.u-tokyo.ac.jp Arabidopsis transcription factors DREB1s/CBFs specifically interact with a cis-acting element DRE/CRT/LTRE involved in the expression of low-temperature-, drought- and high-salinity-inducible genes. Expression of the DREB1 genes is kept at very low level and regulated by circadian control, but induced rapidly and significantly by low temperature. In this study, we analyzed the molecular mechanisms of DREB1 gene expression under both low temperature and circadian control conditions. Using a GUS reporter gene fused to a DREB1C promoter, we identified a region including cis-acting elements involved in regulation of gene expression under low temperature and circadian control conditions. We isolated cDNAs encoding the transcriptional factors, PIF7 and CAMTA2, which specifically bind to the region 80 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
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Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 51 and 52: Session 13.Pathogen defense and pla
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 93 and 94: A B S T R A C T B O O K - L I S T O
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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1. Front Cover.cdr - CORE

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