1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
1. Front Cover.cdr - CORE
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A B S T R A C T B O O K – A B S T R A C T S O F T A L K S<br />
Session 1<strong>1.</strong>Climate change and adaptation to abiotic stress<br />
FUNCTIONAL ANALYSIS OF NOVEL MEMBRANE PROTEIN FAMILY, COR413<br />
CONTROLLED BY AN ARABIDOPSIS TRANSCRIPTION FACTOR DREB1A<br />
Motoki Kanai, Kyonoshin Maruyama, Koji Yamada, Satoshi Kidokoro, Kazuo Shinozaki,<br />
Kazuko Yamaguchi-Shinozaki<br />
Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan<br />
E-mail: aa097014@mail.ecc.u-tokyo.ac.jp<br />
When plants are exposed to various abiotic stresses, they respond and adapt to these<br />
stresses. DREB1A/CBF3 is one of cold-inducible genes encoding transcription factors in<br />
Arabidopsis. Overexpression of DREB1A caused increased tolerance to cold stress in<br />
Arabidopsis, indicating that the DREB1A protein functions in cold stress-responsive gene<br />
expression. Many protein products regulated by DREB1A are probably responsible for the<br />
stress tolerance of plants.<br />
In this study, we analyzed genes for novel membrane protein family, COR413s whose<br />
expression is regulated by DREB1A. We confirmed that the COR413 genes, COR413-IM1,<br />
COR413-IM2.1, and COR413-PM1 were cold inducible by using RNA gel blot analysis. We<br />
observed the subcellular localization patterns of the proteins by using GFP fusion genes.<br />
COR413-IM1 and COR413-IM2.1 were localized at the chloroplast membrane, while<br />
COR413-PM1 was thought to be localized at ER. We analyzed phenotypes of T-DNA<br />
insertion mutants of these genes. There is little difference in cold tolerance between WT<br />
plants and T-DNA insertion mutants. However we found that cor413-im1 and cor413-im2.1<br />
mutants accumulated more anthocyanin as compared to the wild-type plants under cold<br />
stress condition. We performed microarray analyses using these mutants.<br />
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