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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S mostly studied in bacteria, catalyzes the synthesis of polyphosphate from ATP and the degradation of polyphosphate. Putative PPK1 have been identified in the genome of Physcomitrella. Our aim is to understand the role of polyphosphate in plants, using Physcomitrella as a model and engineer higher plants expressing PPK1 to create a new form of P storage. These plants may serve in the phytoremediation of excess phosphate. Two putative PPK1 genes (PpPPK1A and PpPPK1B) were isolated from Physcomitrella. PPK1A and PPKB encode proteins of 90 and 98 kDa, respectively. In order to biochemically characterize the proteins and confirm their function purification of recombinant proteins in E.coli is under way. To further understand polyphosphate metabolism, gene expression was analyzed in Physcomitrella under various growth conditions. Arabidopsis overexpressing PPK1s are currently being analyzed for polyphosphate content, and phosphate metabolism. In order to analyze polyphosphate levels in plants we have successfully purified the recombinant E.coli PPK1 using a polyHis-tag. DEHYDRIN IN BUCKWHEAT SEEDS Michiko Momma National Food Research Institute, Tsukuba, Ibaraki, Japan E-mail: michiko@affrc.go.jp Dehydrin is a class of LEA proteins that are expressed in the late stage of seed maturation in response to water stress and formation of abscisic acid. While involvement of dehydrin in the desiccation tolerance of plants has been extensively studied, little is clarified about the property and function of dehydrin proteins in seeds. We have shown protective activity of dehydrin proteins in soybean and rice seeds to the freezes/thaw denaturation of enzyme proteins. Studies also suggested that the accumulation of dehydrin in peanut relate to human allergenic response. In this study, the presence of dehydrin proteins in buckwheat was indicated for the first time, using immunoblotting for antibody against its highly conserved lysine-rich motif. A major (20 kDa) and a minor (16 kDa) band were found in buckwheat flours, noodles and boiled water used for noodle cooking. The recognition of the major dehydrin protein (20 kDa) was difficult in the SDS-PAGE analysis, since its molecular weight was very close to that of legumin, most abundant storage protein of buckwheat. The minor dehydrin-like protein at 16 kDa showed moderate resistance to pepsindigestion. Results suggested that buckwheat and its product contain dehydrin-like proteins, which associated with pepsin resistance. METABOLIC PROFILING COMBINED WITH MULTIVARIATE STATISTICAL ANALYSIS CAN BE APPLICABLE FOR THE DISCRIMINATION OF SALT TOLERANCE OF RICE (ORYZA SATIVA L.) CULTIVARS. Yu Ran Kim 1 , In Sun Yoon 2 , Taek Ryun Kwon 2 , Hyun Ju Kim 1 , Eunjung Bang 1 , Myung Hee Nam 1 1 Korea Basic Science Insitute, Yuseong-gu, Daejeon, South Korea 2 Department of Agricultural Biotechnology, National Academy of Agricultural Science, Gwonseon gu, Suwon, South Korea E-mail: nammh@kbsi.re.kr Plant responses to salinity involve changes in the activity of genes and proteins, which consequently lead to the changes of metabolism. Metabolome represents final phenotype of cells caused by environment or gene expression. We conducted metabolic profiling approach to screen and characterize genetic diversity of salt tolerance on the metabolite level. For this purpose, we selected 40 kinds of salt resistant and salt sensitive rice cultivars according to their physiological response to NaCl. Low concentration of NaCl was treated for 13 days. Metabolic profiling was conducted with NMR spectroscopy. The partial least squares discriminant analysis (PLS-DA) of the 1H-NMR spectra showed a clear discrimination between salt exposed and control groups. The discrimination between salt sensitive and salt resistant cultivars under salt untreated condition could also obtained by PLS-DA analysis. Major peaks in 1H-NMR spectra contributing to the discrimination were assigned as choline, allantoin, sugars and amino acids like alanine, gutamine, glutamateand aspartate. These results represent that metabolome analysis can be applicable to the discovery of specific phenotype between genetic variations concerning salt tolerance. 55 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S ENHANCING METHIONINE ACCUMULATION IN TOBACCO SEEDS EXPOSES A PHYSIOLOGICAL LINK BETWEEN METHIONINE AND GLUTATHIONE AND ITS IMPACT ON SEED GERMINATION Itamar Godo, Ifat Matityahu, Rachel Amir 1 Laboratory of Plant Science, Migal Galilee Technology Center, Kiryat Shmona, Israel 2 Tel Hai Collage, Upper Galilee, Israel E-mail: rachel@migal.org.il Methionine is an essential amino acid whose level limits the nutritional value of crop plants; however, to date, efforts to increase its content had limited success. We used tobacco plants to express a feedback insensitive Arabidopsis cystathionine γ-synthase (AtCGS), the first committed enzyme in the methionine biosynthesis pathway, under a seed specific promoter. Both methionine and total proteins contents of the transgenic seeds were significantly increased, suggesting that CGS is a rate-limiting enzyme of methionine synthesis in seeds, and that methionine availability limits seed protein synthesis. AtCGS expression altered the protein profile and enhanced the expression of several methionine rich proteins. In the transgenic seeds the amounts of glutathione (GSH) and cysteine, a precursor for methionine and GSH, were reduced, suggesting that methionine accumulates at the expense of GSH. Although these methionine -enriched, low GSH seeds, germinated slower than wild type, adult plants had no phenotype indicating that this effect is limited to seeds. The delayed germination could be reversed by application of GSH. In addition to an important step toward the development of high methionine food crops, this work contributes to the understanding of methionine metabolism, its interactions with GSH and cysteine metabolism and their roles in seed germination. GENETIC ENGINEERING OF CAROTENOIDS BIOSYNTHESIS IN PHAEODACTYLUM TRICORNUTUM Ulrike Eilers, G. Sandmann, C. Büchel Goethe-Universität Frankfurt, Frankfurt am Main, Germany E-mail: u.eilers@sciencemail.org Carotenoids are produced by all photosynthetic organisms and take part in light harvesting as well as in photoprotection. Their antioxidative character and other functions are reasons for their important role in the humans' nutrition. The high costs of synthetic carotenoid production give reason to find suitable hosts for natural biosynthesis. For a proof-of-principle we use the model organism Phaeodactylum tricornutum, a unicellular diatom occurring in salt and brackish water. Since rather little is known about the enzymes involved in carotenoid biosynthesis in diatoms we focus here on four endogenous genes, (Psy, Zep1- 3), coding for phytoensynthase and zeaxanthin-epoxidases 1-3, respectively. Psy catalyses the entry and limiting reaction of the carotenoids biosynthesis. Zep1-3 are coding for the enzymes responsible for the conversion of zeaxanthin to violaxanthin.To investigate these four putative enzymes in P. tricornutum we cloned cDNA and genomic DNA in order to identify their functions by complementing systems in E. coli.An interesting step for industrial purposes is the carotenoid over expression. Therefore we will transform P. tricornutum with an inducible Psy-construct to overcome the limiting entry reaction.Moreover, an additional gene (bkt), coding for a ketolase, is cloned to set a bypass for astaxanthin synthesis without disturbing the physiology. A GUIDELINE TO FAMILY-WIDE COMPARATIVE STATE-OF-THE-ART QRT-PCR ANALYSIS EXEMPLIFIED WITH A BRASSICACEAE CROSS-SPECIES SEED GERMINATION CASE STUDY Ada Linkies, Kai Graeber, Andrew Wood, Gerhard Leubner-Metzger Albert-Ludwigs-University Freiburg, Faculty of Biology, Plant Physiology, Freiburg, Germany E-mail: ada.linkies@biologie.uni-freiburg.de qRT-PCR is a sensitive method to quantify gene expression. Even small differences in expression profiles can be determined, given that the right precautions during data analysis are taken. One major concern is 56 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
Page 5 and 6: Program of the Congress gram of the
Page 7 and 8: A B S T R A C T B O O K - P R O G R
Page 9 and 10: A B S T R A C T B O O K - P R O G R
Page 11 and 12: Plenary session 1 A B S T R A C T B
Page 13 and 14: Plenary session 2 THE BIOLOGY OF AL
Page 15 and 16: Session 1. Biotechnology and synthe
Page 17 and 18: Session 1. Biotechnology and synthe
Page 19 and 20: Session 2. Systems biology and -omi
Page 21 and 22: Session 2. Systems biology and -omi
Page 23 and 24: A B S T R A C T B O O K - A B S T R
Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
Page 35 and 36: Session 6.Organelle biology A B S T
Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
Page 51 and 52: Session 13.Pathogen defense and pla
Page 53 and 54: Session 13.Pathogen defense and pla
Page 55: A B S T R A C T B O O K - A B S T R
Page 67 and 68: Sapporo Medical University, Sapporo
Page 91 and 92: SCF = A B S T R A C T B O O K - A B
Page 93 and 94: A B S T R A C T B O O K - L I S T O
Page 95 and 96: A B S T R A C T B O O K - L I S T O
Page 97 and 98: Sponsors onsors ors A B S T R A C T
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