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A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S OZONE-INDUCED CHANGES IN CAROTENOIDS AND CHLOROPHYLLS IN THREE POPULUS CLONES Sarita Keski-Saari, Jennifer Dumont, Markku Keinänen, Sari Kontunen-Soppela, Elina Oksanen, Didier Le Thiec University of Eastern Finland, Joensuu, Kuopio and Savonlinna, Finland E-mail: sarita.keski-saari@uef.fi Ozone is a phytotoxic air pollutant causing oxidative stress. We studied the effect of ozone on carotenoids, chlorophylls and polyisoprenoid alcohols in three euramerican poplar clones (Populus deltoides x Populus nigra : 'Carpaccio', 'Cima' and 'Robusta'). Poplars originating from cuttings were grown for 6 weeks and exposed to ozone in fumigation chambers (120 ppb each day for 13h). Leaf samples were collected 2, 4, 11, 15 and 17 days after the start of ozone treatment. Chemical analyses were made by HPLC-APCI-MS. The clones clearly differed in growth rate and ozone responses. 'Carpaccio' had the highest above-ground biomass, and it was also the most ozone tolerant genotype. Greatest growth reductions by ozone were measured for 'Cima ', while 'Robusta' showed the greatest number of visible injuries. Ozone and control samples were clearly separate in PCA based on analysed chemical profiles, so that ozone treatment explained 50% of the variance. Ozone reduced the concentrations of chlorophylls a and b, β-carotenes, luteins, neoxanthin and some unidentified isoprenoid derivatives in all clones. The difference between the ozone and control treatments was the strongest on the last two sampling dates. The genotypes differed from each other, but genotype explained only 12% of the variation. THE FIRST BURST OF ROS IN STRESS-INDUCED BIPHASIC GENERATION OF ROS AND ETHYLENE IS AN IMPORTANT DETERMINANT FOR STRESS TOLERANCE Na Ri Ji, Myung Hee Nam, Ky Young Park Sunchon National University, Jeollanam-do, South Korea E-mail: plpm@sunchon.ac.kr The biphasic generation of ROS and ethylene, which was peaked twice in a first transient phase and a second massive phase was occurred after treatment of abiotic/biotic stresses. The activation of both members of NADPH oxidase, RbohD and RbohF, was observed at both phases of ROS production, with much more rapid and abundant increase of RbohD transcripts compared to that of RbohF in first phase. The treatment of ACC was induced more specifically the expression of RbohD in first phase of ROS production. Also, the treatments of AVG and NBD, which are inhibitors of ethylene biosynthesis and ethylene signaling, respectively, were significantly reduced ROS accumulation during phase I. The accumulation of ROS and cell damage, which was significantly decreased after abiotic stresses in transgenic plants, in which ethylene biosynthetic pathway or signaling was impaired. This means that after onset of stress treatment the first peak of endogenous ROS accumulation was followed by ethylene production during phase I, and then ROS and ethylene biosynthesis were occurred for a larger and more prolonged response with necrosis during phase II. Therefore, the first burst of ROS is more responsible for making a role as a signaling molecule during stress-induced response. PHOTOSYNTHETIC RESPONSES OF 3 BIRCH SPECIES TO ELEVATED CO2 CONCENTRATION WITH FACE SYSTEM IN NORTHERN JAPAN Makoto Watanabe, Qiaozhi Mao, Eka Novriyanti, Hirotaka Ito, Kentaro Takagi, Kaichiro Sasa, Takayoshi Koike Hokkaido University, Sapporo, Japan E-mail: nab0602@for.agr.hokudai.ac.jp The increasing CO2 concentration ([CO2]) is one of the most critical environmental changes. Since CO2 is the primary substrate for photosynthesis, the increased [CO2] will act as a fertilizer. However, a decrease in photosynthetic activity, called 'down-regulation of photosynthesis', is occasionally observed. In general, the 77 X X I V S P P S C O N G R E S S 2 0 1 1
A B S T R A C T B O O K – A B S T R A C T S O F P O S T E R S responses to elevated [CO2] are different between species. We examined photosynthetic responses of 3 kinds of birches to elevated CO2 under 2 soil types. Two-year-old seedlings of Betula ermanii, B. maximowicziana and B. platyphylla var. japonica were grown on fertile brown forest soil or infertile volcanic ash soil and exposed to 500 µmol CO2 mol -1 in a free air CO2 enrichment system located in northern Japan for one growing season of 2010. The exposure to elevated [CO2] increased light-saturated net photosynthetic rates at growth [CO2] in the leaves of B. ermanii and B platyphylla, whereas no photosynthetic enhancement was observed in B. maximowicziana. The decrease in maximum rates of carboxylation and electron transport due to the decreasing leaf N content was a factor of down-regulation of photosynthesis in B. maximowicziana under elevated [CO2]. Our results suggest that there is a difference of photosynthetic response to elevated [CO2] even within the same genus of Betula. DEHYDRIN IN BUCKWHEAT SEEDS Michiko Momma National Food Research Institute, Tsukubi, Ibaraki, Japan E-mail: michiko@affrc.go.jp Dehydrin is a class of LEA proteins that are expressed in the late stage of seed maturation in response to water stress and formation of abscisic acid. While involvement of dehydrin in the desiccation tolerance of plants has been extensively studied, little is clarified about the property and function of dehydrin proteins in seeds. We have shown protective activity of dehydrin proteins in soybean and rice seeds to the freezes/thaw denaturation of enzyme proteins. Studies also suggested that the accumulation of dehydrin in peanut relate to human allergenic response. In this study, the presence of dehydrin proteins in buckwheat was indicated for the first time, using immunoblotting for antibody against its highly conserved lysine-rich motif. A major (20 kDa) and a minor (16 kDa) bands were found in buckwheat flours, noodles and boiled water used for noodle cooking. The recognition of the major dehydrin protein (20 kDa) was difficult in the SDS-PAGE analysis, since its molecular weight was very close to that of legumin, most abundant storage protein of buckwheat. The minor dehydrin-like protein at 16 kDa showed moderate resistance to pepsindigestion. Results suggested that buckwheat and its product contain dehydrin-like proteins, which associated with pepsin resistance. EFFECT OF PHOSPHORYLATION ON FUNCTION OF RICE CPD PHOTOLYASE Mika Teranishi, Kentaro Nakamura, Haruya Furukawa, Masaaki Takahashi, Jun Hidema Graduate School of Life Sciences, Tohoku University, Sendai, Tōhoku, Japan E-mail: tera@ige.tohoku.ac.jp Ultraviolet-B (UVB) radiation induces photodamage in DNA, including the formation of cyclobutane pyrimidine dimers (CPDs). CPDs are repaired by CPD photolyase that monomerizes dimers by using blue/UVA light as the energy source. In rice, CPD photolyase is a crucial factor for determining UVB sensitivity. We previously demonstrated that the native rice CPD photolyase was phosphorylated [1]. There is no report that CPD photolyase is phosphorylated in vivo in organism other than rice plant. First, we analyzed the phosphorylation status of CPD photolyase in several poaceous species. It was found that the phosphorylation of CPD photolyase was not a common event among plant species, wheat CPD photolyase was phosphorylated, whereas barley and maize CPD photolyase were not. We also found that CPD photolyase localized to chloroplasts, mitochondria and nuclei [2]. Mitochondria showed a high proportion of non-phosphorylated CPD photolyase, whereas nuclei and chloroplasts had a relatively high proportion of the phosphorylated form. These results suggested that phosphorylation of CPD photolyase was involved in its translocation to organelles. In this congress, we discuss about the significance of phosphorylation on CPD photolyase in rice plants. References [1] Teranishi et al., Plant Physiol. 2008 [2] Takahashi et al., Plant J. 2011 78 X X I V S P P S C O N G R E S S 2 0 1 1
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Foreword eword ord A B S T R A C T
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Program of the Congress gram of the
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A B S T R A C T B O O K - P R O G R
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A B S T R A C T B O O K - P R O G R
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Plenary session 1 A B S T R A C T B
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Plenary session 2 THE BIOLOGY OF AL
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Session 1. Biotechnology and synthe
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Session 1. Biotechnology and synthe
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Session 2. Systems biology and -omi
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Session 2. Systems biology and -omi
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A B S T R A C T B O O K - A B S T R
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A B S T R A C T B O O K - A B S T R
Page 27 and 28: Pleanary session 4 A B S T R A C T
Page 29 and 30: Invited talk 4 A B S T R A C T B O
Page 31 and 32: Session 5.Cell biology A B S T R A
Page 33 and 34: Session 6.Organelle biology A B S T
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Page 37 and 38: Session 7.Development A B S T R A C
Page 39 and 40: Session 8.Signalling A B S T R A C
Page 41 and 42: Pleanary session 6 A B S T R A C T
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Page 67 and 68: Sapporo Medical University, Sapporo
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Page 97 and 98: Sponsors onsors ors A B S T R A C T
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